Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Prevalence of Toxin Genes and Profiles of Antibitoc Resistance in Vibrio vulnificus Isolates from Fish, Fish Tanks, and Patients

어류, 수족관수 및 환자에서 분리된 Vibrio vulnificus의 독소유전자 분포 및 항생제 내성

  • Yoon, Yeon-Hee (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Park, Sook (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Kim, Jin Young (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Lee, Ye Ju (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Jeon, Doo-Young (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Choi, Gyeong Cheol (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Park, Jong Soo (Microbiology Division, Health and Environment Research Institute of Jeollanam-Do) ;
  • Kim, Jung-Beom (Department of Food Science and Technology, Sunchon National University)
  • 윤연희 (전라남도보건환경연구원 미생물과) ;
  • 박숙 (전라남도보건환경연구원 미생물과) ;
  • 김진영 (전라남도보건환경연구원 미생물과) ;
  • 이예주 (전라남도보건환경연구원 미생물과) ;
  • 전두영 (전라남도보건환경연구원 미생물과) ;
  • 최경철 (전라남도보건환경연구원 미생물과) ;
  • 박종수 (전라남도보건환경연구원 미생물과) ;
  • 김중범 (순천대학교 식품공학과)
  • Received : 2019.11.24
  • Accepted : 2019.12.10
  • Published : 2020.02.28
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Abstract

Prevalence of toxin genes and profiles of antibiotic resistance in Vibrio vulnificus were investigated for prevention of Vibrio sepsis and selection of effective antibiotics. A total of 23 V. vulnificus strains were isolated from Vibrio sepsis patients, fish, and water samples collected from fish tanks in restaurants in Jeonnam province during 2015-2017 period. Prevalence of toxin genes including, RtxA, viuB and vvhA were assessed and susceptibilities to 15 different antibiotics were determined. As a result of the toxin gene profile, the RtxA toxin gene was detected in 19 (82.6%) out of 23 strains, and vvhA and viuB toxin genes were positive in all strains. These results showed that V. vulnificus tested in this study possessed at least one more toxin gene, and the toxin gene detection rate was higher than in previous reports. Therefore, there is always a risk of Vibrio sepsis through eating fish or having contact with aquarium water at seafood restaurants. Especially, it was deemed necessary to provide preventive education about Vibrio sepsis for workers in such restaurants. The results of antibiotic susceptibility tests presented 94.4% resistance to cepoxitin antibiotics but all strains showed susceptibility to 14 kinds of antibiotics including chloramphenicol and tetracycline. The currents antibiotic therapy using chloramphenicol and teteracycline against Vibrio sepsis was judged to be useful.

본 연구에서는 비브리오 패혈증 예방과 치료에 유용한 항생제를 제시하기 위해 V. vulnificus의 독소유전자 분포와 항생제 내성을 분석하였다. 2015년부터 2017년까지 3년간 전남지역에서 발생한 비브리오 패혈증 환자로부터 분리되어 보관된 18균주와 전남지역에서 채취된 어패류 및 횟집 수족관수에서 분리된 5균주, 총 23균주를 대상으로 하였다. 실험에 사용된 V. vulnificus 23균주 모두 V. vulnificus로 재확인되었다. V. vulnificus 균주의 독소유전자를 분석한 결과, 23균주 중 19균주(82.6%)에서 RtxA 독소유전자가 확인되었고, 23균주 모두에서 viuB와 vvhA 독소유전자가 검출되었다. 이러한 결과는 독소유전자의 검출율이 기존 보고에 비해 높은 것이며, 실험에 사용한 모든 V. vulnificus 균주가 1개 이상의 독소유전자를 보유한 것으로 생선회 섭취와 상처를 통한 비브리오 패혈증 감염의 위험성이 상존하고 있었다. 따라서 횟집 종사자 등에 대한 비브리오 패혈증 예방 교육이 필요한 것으로 판단되었다. V. vulnificus에 대한 항생제 내성 실험결과 cefoxitin 항생제에 94.4%가 내성을 나타내었고, chloramphenicol과 tetracycline 등 14종의 항생제에 감수성을 나타내었다. 비브리오 패혈증 치료에 chloramphenicol과 tetracycline 항생제를 사용하는 현 치료법이 유용한 것으로 판단되었다.

Keywords

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