The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-inflammatory and Anti-oxidant Studies of Osung-tang Extracts in LPS-Induced RAW 264.7 Cells

LPS로 유도된 RAW 264.7 세포에서 오성탕(五聖湯) 추출물의 항염증 및 항산화 연구

  • Kim, So-Jeong (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University) ;
  • kim, Tae-Jun (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. Of Acupoint & Meridian, College of Korean Medicine, Semyung University) ;
  • Kim, Yong-Min (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
  • 김소정 (세명대학교 화장품뷰티생명공학부) ;
  • 김태준 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실) ;
  • 김용민 (세명대학교 화장품뷰티생명공학부)
  • Received : 2020.01.12
  • Accepted : 2020.02.03
  • Published : 2020.02.25
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Abstract

Objectives : The purpose of this study was to investigate the anti-inflammatory and anti-oxidative effects of Osung-tang(OST) extract. Methods : MTT assay was performed to confirm the survival rate of RAW 264.7 cells treated with OST extract(50-400㎍/㎖) and the production of NO from LPS-induced RAW 264.7 cells was confirmed. The mRNA expression of iNOS, COX-2, and NF-κB were measured by real-time PCR. The protein expression of iNOS, COX-2 and p-IκB were measured by western blot and the anti-oxidant activity of OST extract(50-400㎍/㎖) was investigated by measuring DPPH scavenging activity. Results : OST extract showed a cell survival rate of 90% or more at 50-400㎍/㎖. The NO production was inhibited in a dose-dependent manner in RAW 264.7 cells treated with LPS on OST treated group. mRNA expression levels of iNOS, COX-2 and NF-κB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). Protein expression levels of iNOS, COX-2 and p-IκB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). It was found that OST has a high free-radical scavenging ability. Conclusions : These results suggest that OST extract can be used as a treatment for various skin diseases by demonstrating its anti-inflammatory and anti-oxidant effects.

Keywords

References

  1. Jew SS, Bae ON, Chung JH. Antiinflammatory Effects of Asiaticoside on Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 cell line. Official Journal of Korean Society of Toxicology. 2003;19(1):33-7.
  2. Medzhitov R. Recognition of Microorganisms and Activation of the Immune Response. Nature. 2007;449(7164):819-26. https://doi.org/10.1038/nature06246
  3. Chen F, Castranova V, Shi X. New insights into the Role of Nuclear Factor-kappaB in Cell Growth Regulation. The American journal of pathology. 2001;159(2):387-97. https://doi.org/10.1016/S0002-9440(10)61708-7
  4. Nathan CF. Secretory Products of Macrophages. The Journal of Clinical Investigation. 1987;79(2):319-26. https://doi.org/10.1172/JCI112815
  5. Kim DH, Park SJ, Jung JY, Kim SC, Byun SH. Anti-inflammatory Effects of the Aqueous Extract of Hwangnyeonhaedok-tangin LPS-activated Macrophage Cells. Kor J. Herbology. 2009;24(4):39-47.
  6. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH. Anti-Inflammatory Effects of Extracts from Ligustrum ovalifolium H. Leaves on RAW264.7 Macrophages. Journal of the Korean Society of Food Science and Nutrition. 2012;41(9):1205-10. https://doi.org/10.3746/JKFN.2012.41.9.1205
  7. Sarkar D, Fisher PB. Molecular Mechanisms of Aging-associated Inflammation. Cancer letters. 2006;236(1):13-23. https://doi.org/10.1016/j.canlet.2005.04.009
  8. Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Ahn NK, et al. Anti-inflammatory Effect of Sargassum coreanum Ethanolic Extract through Suppression of $NF-{\kappa}B$ Pathway in LPS Induced RAW264.7 Cells in Mice. Microbiol. Biotechnol. Lett. 2015;43(2):112-9. https://doi.org/10.4014/mbl.1504.04008
  9. Yi HS, Heo SK, Yun HJ, Choi JW, Jung JH, Park SD, Anti-oxidative and Antiinflammatory Effects of Draconis Resina in Mouse Macrophage Cells. The Korea Journal of Herbology. 2008;23(2):179-92.
  10. Woo MO, Park HJ. A Study of the Efficacy of Physiological Activities of Herbal Materials for Cosmetics in 'Sanrimkyoungje'. Journal of Investigative Cosmetology. 2013;9(4):389-404.
  11. Kang HG, Choi BT, Kim YH, Kang HS, Kim HD, Ko WS. The Anti-Inflammatory Effects of Takrisodok-eum in the Ong-Juh. J Korean Oriental Med. 2000;21(1):45-52.
  12. Park MC, Hong SU. The Effects of Sunbanghwalmyung-eum Extract on Anti-oxidant, Anti-allergic and Anti-inflammatory ability in mouse cell. J Korean Med Ophthalmol Otolaryngol Dermatol. 2008;21(2):46-53.
  13. Heo J. Donguibogam. Hadong:Donguibogam Publisher. 2006:1539-82.
  14. Ko WS, Kim KJ, Kim NK, Kim YB, Kim JH, Kim HT, et al. Text of Traditional Korean Dermatology & Surgery. Busan:Seonu. 2007:143-60.
  15. Kim TJ, Kim YM, Kim HT. Effects of Naetakcheongeum-san on Anti-inflammatory Activities in RAW 264.7 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2018;31(1):12-21. https://doi.org/10.6114/jkood.2018.31.1.012
  16. Kim JH, Kim TJ, Kim EH, Kim YM. Inhibitory effects of Wisaengtang on Inflammatory mediators in LPS-induced RAW264.7 cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 2019;32(3):48-57. https://doi.org/10.6114/JKOOD.2019.32.3.048
  17. Korean pharmacology Textbook Editing Board. Korean pharmacology. Seoul: Shinilbooks. 2010:134-786.
  18. Kim NY, Kim HJ, Lee JH, Lee EK, Kang OH, Kwon DY, et al. Comparison of the Anti-inflammatory Effects of Water Fermented and Ethanol Fermented Extracts from Rhei Radix et Rhizoma. Korean J. Oriental Physiology & Pathology. 2011;25(2):227-33.
  19. Shizuo A, Kiyoshi T. Toll-like receptor signalling. Nature Reviews Immunology. 2004;4(7):499-511. https://doi.org/10.1038/nri1391
  20. Lee TH, Kwak HB, Lee ZH, Chung DK, Baek NI, Kim J. Methanol extracts of Stewartia koreana inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by blocking NF-kappa B transactivation in LPS-activated RAW 264.7 cells. Molecules and cells. 2007;23(3):398-404.
  21. Jeon SH, Seol GH, Suh SH, Park SH. Nitric Oxide-Induced Intracellular Ca2+ Modulation in Macrovascular Endothelial Cells. Korean Circulation Journal. 2004;34(6):600-9. https://doi.org/10.4070/kcj.2004.34.6.600
  22. Seo JS, Lee TH, Lee SM, Lee SE, Seong NS, Kim JY. Inhibitory Effects of Methanolic Extracts of Medicinal Plants on Nitric Oxide Production in Activated Macrophage RAW 264.7 cells. Korean J. Medicinal Crop Sci. 2009;17(3):173-8.
  23. Balzary RW, Cocks TM. Lipopolysaccharide induces Epithelium- and Prostaglandin E(2)-Dependent Relaxation of Mouse isolated trachea through Activation of Cyclooxygenase (COX)-1 and COX-2. The Journal of pharmacology and experimental therapeutics. 2006;317(2):806-12. https://doi.org/10.1124/jpet.105.097634
  24. FitzGerald G.A. COX-2 and beyond: Approaches to Prostaglandin Inhibition in Human Disease. Nature Reviews Drug Discovery. 2003;2(11):879-90. https://doi.org/10.1038/nrd1225
  25. Lee DH, Sohn DS, Cho DY, Kim BJ, Lim YY, Kim YH. Anti-inflammatory and Antioxidant Effects of Sophora flavescens Root Extraction in Lipopolysaccharide-activated Raw 264.7 Cells. Korean journal of Medical Mycology. 2010;15(2):39-50.
  26. Mun SI, Ryu HS, Lee HJ, Choi JS. Further Screening for Antioxidant Activity of Vegetable Plants and Its Active Principles from Zanthoxylum schinifolium. The Korean juounal of food and nutrition. 1994;23(3):466-71.
  27. KDA Textbook Editing Board. Dermatology 5th edition. Seoul:Ryo Moon Gak. 2008:165-77.
  28. Ahn SH, Kim HH. Lonicera Japonica Inhibits Atopy Dermatitis in NC/Nga Mouse through Regulation of iNOS by $NF-{\kappa}B$ Suppression. Korean J. Oriental & Pathology. 2010;24(2):278-83.