The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Gammakdaejo-tang(GMD) on DNCB induced Atopic Dermatitis in Mice

감맥대조탕이 DNCB로 유발된 생쥐의 아토피피부염에 미치는 영향

  • Ryu, Ji-Yeon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kam, Eun-Young (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kang, Eun-Jeong (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Choi, Jung-Hwa (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kim, Jong-Han (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Park, Soo-Yeon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Jung, Min-Yeong (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
  • 류지연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 감은영 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 강은정 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 김종한 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2020.03.31
  • Accepted : 2020.05.12
  • Published : 2020.05.25
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Abstract

Objectives : Atopic dermatitis is a chronic inflammatory skin disease with frequent relapses. This study was to investigate the effects of Gammakdaejo-tang(GMD) in DNCB induced atopic dermatitis mice. Methods : The study was divided into five comparion groups. 2,4-dinitrochlorobenzene(DNCB) solution was applied to Nc/Nga mice to induce atopic dermatitis, followed by normal group, negative control group with distilled water, positive control group with Dexamethasione and GMD 200mg/kg or 400mg/kg. The control group was orally administered 200㎕ once daily for 4 weeks. Visual skin condition, Immunoglobulin E, Histamine, Cytokine, Immune cells, Tissue biomarkers were observed. Results : As a result of the dermatitis score evaluation, it was confirmed that the GMD-administered group improved symptoms compared to the negative control group. As a result of measuring IgE, the GMD-administered group significantly decreased compared to the negative control group. As a result of measuring Histamine, GMD group except 200mg/kg of GMD significantly decreased compared to negative control group. As a result of measuring cytokine, GMD 200mg/kg significantly reduced IL-1β, IL-6 and TNF-α compared to the negative control. 400mg/kg significantly reduced IL-1β, IL-4, IL-5, IL-6, IL-10, TNF-α and significantly increased IL-2, IFNγ. As a result of confirming the immune cells, all experimental groups showed no difference in basophil, GMD group significantly reduced monocyte and eosinophil compared to negative control group, and GMD 400mg/kg group significantly reduced white blood cell and neutrophil. And significantly increased lymphocytes. As a result of measuring the gene expression level, all GMD group significantly increased TGF-β1 compared with the negative control group, and filaggrin, VEGF and EGF were significantly increased in GMD 400mg/kg group. Epidermis, dermis thickness, and eosinophil infiltration were found to be decreased in all GMD groups compared with the negative control group. Conclusions : GMD is effective in atopic dermatitis by reducing imbalance of immune response of T cells (Th1 / Th2) and reducing skin tissue damage and inflammatory response.

Keywords

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