Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Distribution and Toxin Gene Characteristic of Bacillus cereus Isolated from Foods in Busan

부산지역 가공식품 중 Bacillus cereus 분포 현황 및 독소 유전자 특성

  • Park, So-hyun (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Gwon, Wi-Gyeong (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Lee, In-sook (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Kim, Eun-ju (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Hwang, Su-jeong (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Koo, Hee-soo (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Na, Young-ran (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Kim, Byung-jun (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Park, Eun-hee (Microbiology Team, Busan Metropolitan City Institute of Health & Environment) ;
  • Lee, Mee-ok (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
  • 박소현 (부산광역시 보건환경연구원 미생물팀) ;
  • 권위경 (부산광역시 보건환경연구원 미생물팀) ;
  • 이인숙 (부산광역시 보건환경연구원 미생물팀) ;
  • 김은주 (부산광역시 보건환경연구원 미생물팀) ;
  • 황수정 (부산광역시 보건환경연구원 미생물팀) ;
  • 구희수 (부산광역시 보건환경연구원 미생물팀) ;
  • 나영란 (부산광역시 보건환경연구원 미생물팀) ;
  • 김병준 (부산광역시 보건환경연구원 미생물팀) ;
  • 박은희 (부산광역시 보건환경연구원 미생물팀) ;
  • 이미옥 (부산광역시 보건환경연구원 미생물팀)
  • Received : 2020.03.02
  • Accepted : 2020.04.22
  • Published : 2020.06.30
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Abstract

The purpose of this study was to provide basic data necessary for the prevention of food poisoning and safe food management. We examined 872 food samples for B. cereus in accordance with the MFDS Food Code and investigated characteristics of their harboring toxin genes. We detected and isolated 113 strains of B. cereus from 78 food samples (8.9%), and the average detection level was 48 CFU/g. B. cereus isolates carried at least 1 toxin gene among the emetic toxins and 5 enterotoxin genes. The toxin gene profiles of B. cereus were classified into 18 different types of isolates showing genetic diversity. Among the strains, 34 (30.1%) had all 5 enterotoxin genes (Cytk-nheA-entFM-bceT-hblC), accounting for the highest percentage. The entFM and nheA genes were major enterotoxin genes, while the emetic toxin gene, CER, was the least detected in B. cereus isolated from food samples.

본 연구는 2018년 10월에서 2019년 9월까지 부산지역 유통 판매되고 있는 872건의 다양한 식품을 대상으로 B. cereus 오염도를 분석하였다. 총 872건 중 78건(8.9%)에서 B. cereus 검출되었으며 식품별 B. cereus 검출률은 김치·절임·조림·젓갈류에서 23.9%, 조미식품 19.4%, 바로 섭취가능한 농산물 10.5%, 조리식품 7.3%, 즉석섭취식품 5.6%, 신선편의식품 5.0%, 즉석조리식품 0.0% 순으로 분포하였으며 통계적으로 유의한 차이가 있었다. B. cereus 오염 수준은 불검출에서 최대 20,000 CFU/g로 평균 48 CFU/g였으며, 식품별 B.cereus 오염도에는 차이가 없었다. 78건의 검체에서 분리된 113주의 B. cereus의 독소 유전자 확인 시험을 수행하였다. 식품에서 분리된 113주를 대상으로 B. cereus 독소유전자 확인 결과 최소 1종류에서 최대 5종류의 독소 유전자가 검출되었으며 총 18개 profile로 분류되었다. 장독소 5종(Cytk-nheA-entFM-bceT-hblC)을 모두 보유한 경우가 34주(30.1%)로 가장 많았다.

Keywords

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