Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Effects of number of frozen-thawed ram sperm and number of inseminations on fertility in synchronized ewes under field condition

  • Jha, Pankaj Kumar (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Alam, Md. Golam Shahi (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Al Mansur, Md. Abdullah (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Talukder, Mohammad Rafiqul Islam (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Naher, Nazmun (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Rahman, A.K.M. Anisur (Department of Medicine, Bangladesh Agricultural University) ;
  • Hal, David C. (Department of Ecosystem and Public Health, University of Calgary) ;
  • Bari, Farida Yeasmin (Department of Surgery and Obstetrics, Bangladesh Agricultural University)
  • Received : 2020.04.13
  • Accepted : 2020.06.09
  • Published : 2020.06.30
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Abstract

The effects of the number of frozen-thawed ram sperm per single and double intra-cervical artificial insemination (AI) on fertility in ewes were studied. A total of 89 non-pregnant ewes were synchronized for oestrus with two doses of 100 ㎍ PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; D200 (n = 23; double AI with 200 × 106 sperm), S200 (n = 24; single AI with 200 × 106 sperm), D100 (n = 24; double AI with 100 × 106 sperm) and S100 (n = 18; single AI with 100 × 106 sperm). Ewes were inseminated within 12 to 18 h for single AI and, within 10 to 12 h and 16 to 18 h for double AI after the onset of oestrus. The onset of oestrus ranged from 28 to 76 h (54.33 ± 1.28 h). The high percentage (29.2%) of ewes showed oestrus between 51 to 60 h. The non-return rates were highest in group D200 (56.5%) and differed significantly (p < 0.05) from group S100 (11.1%). No ewes were pregnant in group S100, and the pregnancy rates among the remaining groups did not differ. The mean gestation period was 152.8 ± 0.5 days and no difference was observed among the groups. The lambing and multiple birth rates were 100% in group D200. The single and twin lambing was highest in group D100 (33.3%) and group D200 (83.3%), respectively. Only one triplet lambing and the highest lambing size (2.2 ± 0.2) was recorded in group D200. In conclusion, double AI with 200 × 106 sperm showed comparatively most practical for achieving high pregnancy rates and lambing performances in Bangladeshi ewes under field conditions.

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References

  1. Acritopoulou-Fourcroy S, Pappas V, Peclaris G, Zervas N, Skoufis V. 1982. Synchronization of oestrus in ewes with Provera sponges/PMSG, prostaglandin F2${\alpha}$ or the prostaglandin analogue, ICI 80996, and fertility following natural mating or artificial insemination. Reprod. Nutr. Develop. 22:345-354. https://doi.org/10.1051/rnd:19820305
  2. Andersen Berg K and Aamdal J. 1991. Artificial insemination with frozen semen in ewes at different times of the breeding season. Reprod. Domest. Anim. 26:27-30. https://doi.org/10.1111/j.1439-0531.1991.tb01508.x
  3. Anel L, Alvarez M, Martinez-Pastor F, Garcia-Macias V, Anel E, de Paz P. 2006. Improvement strategies in ovine artificial insemination. Reprod. Domest. Anim. 41 Suppl 2:30-42. https://doi.org/10.1111/j.1439-0531.1967.tb01206.x
  4. Anel L, de Paz P, Alvarez M, Chamorro CA, Boixo JC, Manso A, Gonzalez M, Kaabi M, Anel E. 2003. Field and in vitro assay of three methods for freezing ram semen. Theriogenology 60:1293-1308. https://doi.org/10.1016/S0093-691X(03)00140-7
  5. Azizunnesa, Zohara BF, Bari F, Alam MGS. 2014. Effects of proportion of egg yolk and preservation time on chilled semen from indigenous rams. GSTF J. Vet. Sci. 1:18-26.
  6. Bhuiyan AKFH. 2006. Livestock genetic resources in Bangladesh: preservation and management. International conference on livestock services, Chinese Academy of Agricultural Science (CAAS), Beijing, China, pp. 16-20.
  7. Branca A, Cappai P, Dattene M, Gallus M, Ledda S, Loi P, Naitana S. 1994. A simplified method of artificial insemination for ewe lambs. In: Abstraacts of the XI Congresso Nazinale Societa Italiana Di Patologia e Di Allevamento Degli Ovini e Di Caprini. Perugia, Italy. pp. 317-318.
  8. D’Alessandro AG, Martemucci AG, Colonna MA, Bellitti A. 2001. Post-thaw survival of ram spermatozoa and fertility after insemination as affected by prefreezing sperm concentration and extender composition. Theriogenology 55:1159-1170. https://doi.org/10.1016/S0093-691X(01)00474-5
  9. Davis A, Fleet I, Harrison F, Walker FM. 1980. Pulmonary metabolism of prostaglandin F2a in the conscious non-pregnant ewe and sow. J. Physiol. 301(Suppl), 86.
  10. Downing JA and Scaramuzzi RJ. 1991. Nutrient effects on ovulation rate, ovarian function and the secretion of gonadotrophic and metabolic hormones in sheep. J. Reprod. Fertil. Suppl. 43:209-227.
  11. Gungor O, Cenesiz M, Pancarci SM, Yildiz S, Kaya M, Kacar C, Ozyurtlu N, Gurbulak K. 2007. Effects of different intravaginal progesterone releasing devices on estrous synchronization and LH surge in fat-tailed ewes during non-breeding season. Med. Weter. 63:1316-1319.
  12. Hashem NM, El-Zarkouny SZ, Taha TA, Abo-Elezz ZR. 2015. Oestrous response and characterization of the ovulatory wave following oestrous synchronization using $PGF2{\alpha}$ alone or combined with GnRH in ewes. Small Rumin. Res. 129:84-87. https://doi.org/10.1016/j.smallrumres.2015.06.003
  13. Hashemi M, Safdarian M, Kafi M. 2006. Estrous response to synchronization of estrus using different progesterone treatments outside the natural breeding season in ewes. Small Rumin. Res. 65:279-283. https://doi.org/10.1016/j.smallrumres.2005.07.051
  14. Jha PK, Alam M, Al-Mansur MA, Islam M, Bari F. 2018. Selection of breeding rams by evaluating semen quality. J. Appl. Anim. Sci. 11:9-20.
  15. Jha PK, Alam MGS, Mansur AAL, Naher N, Islam T, Bhuiyan MU, Bari FY. 2019. Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques. Cryobiology 89:35-41. https://doi.org/10.1016/j.cryobiol.2019.06.001
  16. Kerton D, McPhee S, Davis I, White M, Banfield J, Cahill L. 1984. A comparison of insemination techniques in Corriedale ewes. Proceedings of the Australian Society of Animal Production. 15, pp.701.
  17. Khalifa TA, Lymberopoulos AG, El-Saidy BE. 2008. Testing usability of butylated hydroxytoluene in conservation of goat semen. Reprod. Domest. Anim. 43:525-530. https://doi.org/10.1111/j.1439-0531.2007.00947.x
  18. Light JE, Silvia WJ, Reid RC 2nd. 1994. Luteolytic effect of prostaglandin F2 alpha and two metabolites in ewes. J. Anim. Sci. 72:2718-2721. https://doi.org/10.2527/1994.72102718x
  19. Martinez-Tinajero JJ, Ruiz-Herluver I, Montanez-Valdez OD, Martinez-Priego G, Velasco-Zebadua ME, Izaguirre F. 2011. Reproductive performance in Dorper ewes synchronized at estrus during non breeding season in tropical conditions. J. Anim. Vet. Adv. 10:221-223. https://doi.org/10.3923/javaa.2011.221.223
  20. Maxwell WM, Landers AJ, Evans G. 1995. Survival and fertility of ram spermatozoa frozen in pellets, straws and minitubes. Theriogenology 43:1201-1210. https://doi.org/10.1016/0093-691X(95)00092-M
  21. Maxwell WMC and Hewitt LJ. 1986. A comparison of vaginal, cervical and intrauterine insemination of sheep. J. Agric. Sci. 106:191-193. https://doi.org/10.1017/S0021859600061906
  22. Meinecke B and Meinecke-Tillmann S. 1986. [Fertilization results in superovulated ewes and goats after transmuralintrauterine insemination controlled by laparoscopy]. Tierarztl. Prax. 14:35-41. German.
  23. Menchaca A, Miller V, Gil J, Pinczak A, Laca M, Rubianes E. 2004. Prostaglandin F2alpha treatment associated with timed artificial insemination in ewes. Reprod. Domest. Anim. 39:352-355 https://doi.org/10.1111/j.1439-0531.2004.00527.x
  24. Menchaca A and Rubianes E. 2004. New treatments associated with timed artificial insemination in small ruminants. Reprod. Fertil. Dev. 16:403-413. https://doi.org/10.10371/RD04037
  25. Moeini MM, Alipour F, Moghadam A. 2009. The effect of human chorionic gonadotropin on the reproduction performance in Lory sheep synchronized with different doses of pregnant mare serum gonadotrophin outside the breeding season. Asian J. Anim. Vet. Adv. 4:9-15. https://doi.org/10.3923/ajava.2009.9.15
  26. Nordstoga AB, Soderquist L, Adnoy T, Paulenz H. 2009. Effect of different packages and freezing/thawing protocols on fertility of ram semen. Reprod. Domest. Anim. 44:527-531. https://doi.org/10.1111/j.1439-0531.2008.01284.x
  27. Olivera-Muzante J, Fierro S, Lopez V, Gil J. 2011. Comparison of prostaglandin- and progesterone-based protocols for timed artificial insemination in sheep. Theriogenology 75:1232-1238. https://doi.org/10.1016/j.theriogenology.2010.11.036
  28. Ozturkler Y, Colak A, Baykal A, Guven B. 2003. Combined effect of a prostaglandin analogue and a progestagen treatment for 5 days on oestrus synchronisation in Tushin ewes. Indian Vet. J. 80:917-920.
  29. Paulenz H, Adnoy T, Fossen OH, Soderquist L. 2009. Effect on field fertility of addition of gelatine, different dilution rates and storage times of cooled ram semen after vaginal insemination. Reprod. Domest. Anim. 45:706-710. https://doi.org/10.1111/j.1439-0531.2008.01338.x
  30. Paulenz H, Adnoy T, Fossen OH, Soderquist L, Berg KA. 2002. Effect of deposition site and sperm number on the fertility of sheep inseminated with liquid semen. Vet. Rec. 150:299-302. https://doi.org/10.1136/vr.150.10.299
  31. Rekha A, Zohara BF, Bari F, Alam MGS. 2016a. Comparison of commercial Triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries). Small Rumin. Res. 134:39-43. https://doi.org/10.1016/j.smallrumres.2015.12.007
  32. Rekha A, Zohara BF, Bari FY, Alam MGS. 2016b. Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep. Anim. Reprod. 13:735-742. https://doi.org/10.21451/1984-3143-AR733
  33. Roy PK, Zohara BF, Nesa A, Paul AK, Bhuiyan MMU, Bari FY. 2014. Oestrus synchronization with Ovuprost$^{(R)}$ and Prostenol$^{(R)}$ in the indigenous ewes of Bangladesh. J. Emb. Trans. 29:149-155. https://doi.org/10.12750/JET.2014.29.2.149
  34. Tekin N, Uysal O, Akcay E, Yavas I. 2006. Effects of different taurine doses and freezing rate on freezing of row semen. Ank. Univ. Vet. Fak. Derg. 53:179-184.
  35. Tervit H, Goold P, James R. 1984. The insemination of sheep with fresh or frozen semen. In: Abstracts of the proceedings of the New Zealand Society of Animal Production. pp. 11-13.
  36. Trounson AO, Willadsen SM, Moor RM. 1976. Effect of prostaglandin analogue Cloprostenol on oestrus, ovulation and embryonic viability in sheep. J. Agric. Sci. 86:609-611. https://doi.org/10.1017/S0021859600061177
  37. Turk G, Gur S, Sonmez M, Bozkurt T, Aksu EH, Aksoy H. 2008. Effect of exogenous GnRH at the time of artificial insemination on reproductive performance of Awassi ewes synchronized with progestagen-PMSG-PGF2alpha combination. Reprod. Domest. Anim. 43:308-313. https://doi.org/10.1111/j.1439-0531.2007.00896.x
  38. Turner HN. 1983. Origins of the CSIRO Booroola. Wool Technol. Sheep Breed. 31:10-13.
  39. Wulster-Radcliffe MC and Lewis GS. 2002. Development of a new transcervical artificial insemination method for sheep: effects of a new transcervical artificial insemination catheter and traversing the cervix on semen quality and fertility. Theriogenology 58:1361-1371. https://doi.org/10.1016/S0093-691X(02)01042-7
  40. Yadi J, Moghaddam MF, Khalajzadeh S, Solati AA. 2011. Comparison of estrus synchronization by $PGF2{\alpha}$, CIDR and sponge with PMSG in Kalkuhi ewes on early anestrous season. In: Abstracts of the International Conference on Asia Agriculture and Animal. Singapoore. pp. 61-65.
  41. Zohara BF, Azizunnesa A, Faruk I, Alam MGS, Bari FY. 2014. Comparison of estrus synchronization by $PGF2\alpha$ and progestagen sponge with PMSG in indigenous ewes in Bangladesh. GSTF J. Vet. Sci. 1:27-37.