Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Detection of Fungicidal Activities against Alternaria dauci Causing Alternaria Leaf Spot in Carrot and Monitoring for the Fungicide Resistance

당근검은잎마름병균 Alternaria dauci에 대한 살균제 효과 검정 및 병원균 집단에 대한 저항성 검정

  • Do, Jiwon (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Min, Jiyoung (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Kim, Yongsu (K. C. Carrot Breeding Institute) ;
  • Park, Yong (K. C. Carrot Breeding Institute) ;
  • Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • 도지원 (충북대학교 농업생명환경대학 식물의학과) ;
  • 민지영 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김용수 (한중당근육종연구소) ;
  • 박용 (한중당근육종연구소) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2020.03.08
  • Accepted : 2020.04.07
  • Published : 2020.06.30
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Abstract

With 32 fungicides, it was examined the inhibitory effects on the mycelial growth of Alternaria dauci KACC42997 causing Alternaria leaf blight of carrot. Showing the results of the agar dilution method, the fungicides belonging to C2, C5, G1, E2, and E3 group were excellent in inhibiting mycelial growth. Protective fungicides belonging to M group, except for iminoctadine tris-albesilate, and pyraclostrobin belonging to C3 group were effective in inhibiting spore germination of pathogens. The fungicides included into C2 group inhibiting succinate dehydrogenase activity and the G1 group inhibiting demethylase activity showed the excellent inhibitory effect on mycelial growth but the inhibitory effect of spore germination was very low. However, fluazinam belonging to C5 group was excellent in inhibiting spore germination as well as mycelial growth. Especially, when 100 ㎍/ml of fluxapyroxad belonging to the C2 group was treated, 47.1% of spore formation was inhibited on the medium. In comparison of the resistance factors of 3 fungicide groups, as G, C, and E group, in populations of A. dauci isolates collected from Gumi, Pyeongchang, and Jeju, resistance factor in the population of Jeju was the lowest. However, two isolates resistant to fludioxonil belonging to E2 group were found in the isolate group of Pyeongchang, and both showed cross-resistance to iprodione and procymidone.

당근검은잎마름병균인 Alternaria dauci KACC42997에 대해서 32개 살균제를 선발하여 균사생장 억제효과를 조사한 결과, '다2'군, '다5'군, '사1'군, '마2'군, '마3'군에 속하는 살균제의 균사생장 억제효과가 우수하였다. Iminoctadine tris-albesilate를 제외한 '카'군에 속하는 보호살균제와 '다3'군에 속하는 pyraclostrobin은 병원균의 포자발아를 억제하는 효과가 우수하였다. 균사생장 억제효과가 우수했던 숙신산탈수소효소 활성을 저해하는 '다2'군과 탈메틸효소 활성을 저해하는 '사1'군 살균제는 우수한 균사생장 억제효과를 보여주면서도, 포자발아 억제효과는 저조하였다. 하지만 '다5'군에 속하는 fluazinam은 균사생장 억제효과뿐만 아니라 포자발아 억제효과도 우수하였다. 특별히 '다2'군에 속하는 fluxapyroxad를 100 ㎍/ml 처리한 경우, 배지 상에서 포자형성을 47.1% 억제하였다. 구미, 평창, 제주 등에서 분리한 검은잎마름병균 집단의 '사'군, '다'군, '마'군 살균제에 대한 저항성 요인값을 비교하면, 제주 지역 병원균 집단의 저항성 요인값이 가장 낮았다. 평창 지역 균주 집단에서 '마2'군에 속하는 fludioxonil 저항성 균주가 2개 발견되었으며, 그들은 모두 iprodione과 procymidone에 대해서 교차저항성을 보였다.

Keywords

References

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