Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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A flowcytometric determination of DNA content in Pacific abalone, Haliotis discus hannai cell

유동세포분석에 의한 참전복(Haliotis discus hannai) 세포내 DNA 함량 분석 최적화

  • Park, In-Seok (Division of Marine Bioscience, Korea Maritime & Ocean University)
  • 박인석 (한국해양대학교 해양생명과학부)
  • Received : 2020.01.28
  • Accepted : 2020.05.14
  • Published : 2020.06.30
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Abstract

The level at which analyses of DNA content might contribute more significantly to the genetic mechanisms of evolution lies in the events of speciation. The object of this study was to investigate the DNA content of abalone (Haliotis discus hannai) and determine the optimal tissue samples for measuring the DNA content of abalone by flowcytometry without fixation. The DNA content (pg/nucleus) of gill tissue (2.5±0.08), which was contaminated with protozoa, was significantly lower than that of muscle tissue (3.2±0.02), mantle tissue (3.2±0.02) (p<0.05), and a standard reference standard, while the DNA contents of muscle tissue and mantle tissue were higher than that of the standard reference. Considering the results of this study, DNA content analysis with flowcytometry is an acute and rapid method by which muscle tissue and mantle tissue are the most appropriate sample for measuring the DNA content of abalone without fixation.

DNA 함량 조사는 진화의 유전적 기작을 예측하여 종분화를 파악하게 한다. 본 연구의 목적은 참전복(Haliotis discus hannai)의 DNA 함량을 측정하고 유세포분석기(flowcytometry)로 고정(fixation) 없이 DNA 함량 측정에 최적인 조직들을 파악하는 것이다. DNA 함량 (pg nucleus-1)에 있어, 원생생물에 오염된 아가미조직(2.5±0.08)은 3.2±0.02 pg nucleus-1의 DNA 함량을 보인 근육조직과 외투막조직에 비하여 유의하게 낮았고(p<0.05) standard reference보다도 낮은 반면, 근육조직과 외투막조직은 standard reference보다 높았다. 본 연구 결과들을 고려 시, 참전복에서 고정 없이 유동세포분석에 적절한 조직은 근육과 외투막이며 본 연구에 적용된 고정 과정이 없는 유세포분석법은 참전복 DNA 함량 분석 시 정확하고, 신속한 방법임이 판명되었다.

Keywords

References

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