DOI QR코드

DOI QR Code

A Study on the Functional Characteristics of Cuscuta australis R.Brown

실새삼(Cuscuta australis R.Brown)의 기능적 특성에 관한 연구

  • Jeong, Kap-Seop (Department of Food Science & Nutrition, TongMyong University)
  • Received : 2020.07.10
  • Accepted : 2020.08.31
  • Published : 2020.08.31

Abstract

To investigate the usefulness of natural plants, physicochemical characteristics, functional components and physiological activities of dodder(Cuscuta australis R.Brown) were experimented. The extraction of dodder was carried out at 60℃ in shaking bath with 10 times of distilled water for three hours. Harmful metals such as lead, cadmium and arsenic were not detected, but the content of mercury from dodder was 0.0004ppm in acceptable standards. The extraction yield was 23.45%, total content of phenolics equivalent to gallic acid was 699.8 mg/100g and total content of flavonoid equivalent to naringin was 373.8 mg/100g. 28 kinds of free amino acids were detected with the contents of 252mg/100g in the dodder extract, and the contents of anserine and sarcosine were analysed highest in all amino acids. From the comparison of physiological activities of dodder extract with those of ascorbic acid, total reducing power, electron donating ability based on DPPH radical scavenging and ferric reducing antioxidant power was equivalent to 88%, 56% and 40%, respectively, of ascorbic acid. The nitrite scavenging activity of dodder extract was 78% of ascorbic acid on pH 1.5, but the antioxidant index of dodder on perilla oil was about 4 times greater than that of ascorbic acid.

천연물 유래 식물소재의 유용성 탐색의 일환으로 자생하는 실새삼을 열수로 추출하여 몇 가지 이화학적 추출특성과 기능성분 함량 및 생리활성을 측정하였다. 실새삼 중 납, 카드뮴 및 비소는 검출되지 않았으며, 수은은 0.0004ppm으로 허용 기준치 이내의 함량으로 검출되었고, 추출수율은 23.45%, gallic acid에 대응되는 총페놀함량과 naringin에 대응되는 총플라보노이드 함량은 각각 699.8과 373.8 mg/100g으로 측정되었다. 실새삼 추출물에서 28종의 유리 아미노산이 검출되었으며, 이 중 anserine과 sarcosine의 함량이 가장 높게 측정되었다. 실새삼 추출물과 ascorbic acid의 생리활성을 비교한 결과 추출물의 총환원력, 전자공여능 및 철환원력은 각각 ascorbic acid의 88%, 56% 및 40%의 크기에 해당하였다. pH 1.5에서 실새삼 추출물의 아질산염 소거능은 ascorbic acid의 78%의 활성을 보였으며, 들기름에 대한 항산화지수는 ascorbic acid의 약 5배에 해당하는 우수한 산화억제 효과를 보여 천연 항산화제로서의 가능성을 보였다.

Keywords

References

  1. J. Y. Oh, U. Choi, Y. S. Kim, D. H. Shin, "Isolation and Identification of Antioxidative Components from Bark of Rhus javanica Linne", Korean J. Food Sci. Technolo., Vol.35, No.4, pp. 726-732, (2003).
  2. S. W. Park, Y. J. Jin, Y. W. Kwon, B. C. Moon, C. S. Kim, J. D. Shin, "Comparison of Physiological and Ecological Characteristics for Dodders (Cuscuta spp.) distriduted in Korea", Kor. J. Weed Sci., Vol.29, No.4, pp. 288-297, (2009).
  3. Y. H. Jung, J. K. Lee, S. Y. Lee, R. H. Jang, S. H. Lee, K. T. Cho, Y. H. You, "Host plant preference, parasitic site and risk possibility of Cuscuta pentagona Engelm, invasive plant in Korea", Korean J. Environ. Ecol., Vol.31, No.3, pp. 287-296, (2017). https://doi.org/10.13047/KJEE.2017.31.3.287
  4. https://100.daum.net/encyclopedia/view/73XX26000176
  5. K. D. Suk, S. J. Lee, J. M. Bae, "Inhibitory effects of Cuscuta japonica extract and C. australis extract on mushroom tyrosinase activity", Kor. J. Pharmacogn., Vol.35, No.4, pp. 380-383, (2004).
  6. S. J. Chang, K. D. Suk, "Inhibitory effects on melanin biosynthesis and tyrosinase activity, cytotoxicity in Clone M-3 and antioxidant activity by Cuscuta japanica, C. australis, and C. chinesis extracts", Yakhak Hoeji, Vol.50, No.6, pp. 421-428, (2006).
  7. C. D. Lee, K. B. Lee, "Ultrastructure of haustorial cells of Cuscuta australis R. Brown", Korean J. electron microscopy, Vol.16, No.2, pp. 49-60, (1986).
  8. J. S. Kim, H. H. Kwak, B. C. Kim, K. Y. Cho, "Study on the biosynthesis characteristics of photosynthetic pigments in dodder(Cuscuta australis R. Br.) plant", Kor. J. Weed Sci., Vol.17, No.3, pp. 314-324, (1997).
  9. K. D. Suk, S. H. Lee, K. S. Kim, "Growth-inhivitory effects of Cuscuta japonica Choisy and C. australis R. Be's extracts against propionibacterium acnes", Kor. J. Pharmacogn., Vol.35, No.4, pp. 375-379, (2004).
  10. Heriyanto, L. Limantatr, "The composition and the content of the main pigments on dodders plant Cuscuta australis R. Br. and Cassytha filiformis L.", Makara, Sains, Vol.10, No.2, pp. 69-75, (2006).
  11. M. O. K. Azad, J. S. Min, C. Y. Lee, I. J. Sung, B. D. Lee, K. J. Chang, C. H. Park, "Total polyphenol and antioxidant activity of Cuscuta australis seeds (Tosaja) and Buckwheat flowers", Academic symposium, Plant Resources Society of Korea, p.176-176, (2016).
  12. R. O. Folarin, J. O. Omirinde, R. Bejide, T. O. Isola, L. I. Usende, A. Basiru, "Comparative hepatoprotective activity of ethanolic extracts of Cuscuta australis against acetaminophen intoxication in wistar rats", Hindawi Pub. Co., pp. 1-6, (2014).
  13. C. Hettenhausen, J. Li, H. Zhuang, H. Sun, Y. Xu, J. Qi, J. Zhang, Y. Lei, Y. Qin, G. Sun, L. Wang, I. T. Baldwin, J. Wu, "Stem parasitic plant Cuscuta australis (dodder) transfers herbivoryinduced signals among plants", PNAS, pp. E6703-E6709, (2017).
  14. K. Helrich, "Official method of analysis", 15th, Association of official analytical chemists, Washington D.D., USA, p.40, (1990).
  15. E. Y. Kim, I. H. Baik, J. H. Kim, S. R. Kim, M. R. Rhyu, "Screening of the antioxidant activity of some medicinal plants", Korean J. Food Sci. Technol., Vol.36, pp. 333-338, (2004).
  16. M. A. Eum, Y. H. Kang, D. S. Kwon, K. S. Jo, "The nitrite scavenging and electron donating ability of potato extracts", Korean J. Food & Nutr., Vol.12, pp. 478-483, (1999).
  17. H. S. Song, Y. H. Park, S. H. Jung, D. P. Kim, Y. H. Jung, M. K. Lee, K. Y. Moon, "Antioxidant Activity of Extracts from Smilax china Root", J. Korean Soc. Food Sci. Nutr., Vol.35, No.9, pp. 1133-1138, (2006). https://doi.org/10.3746/jkfn.2006.35.9.1133
  18. S. Burda, W. Oleszek, "Antioxidant and antiradical activities of flavonoids", J. Agric. Food Chem., Vol.49, 2774-2779, (2001). https://doi.org/10.1021/jf001413m
  19. I. F. F. Benzie, J. J. Strain, "The ferric reducing ability of Plasma(FRAP) as a measure of antioxidant power : The FRAP assay", Anal. Biochem., Vol.239, pp. 70-76, (1996). https://doi.org/10.1006/abio.1996.0292
  20. Y. H. Kang, Y. K. Park, G. D. Lee, "The nitrite scavenging and electron donating ability of phenolic compounds", Korean J. Food Sci. Technol., Vol.28, pp. 232-239, (1996).
  21. https://www.foodsafetykorea.go.kr/foodcode/01_03.jsp?idx=12
  22. J. S. Hwang, B. H. Lee, X. An, H. R. Jeong, Y. E. Kim, I. Lee, H. Lee, D. O. Kim, "Total phenolics, total flavonoids, and antioxidant capacity in the leaves, bulbs, and roots of Allium hookeri", Korean J. Food Sci. Technol., Vol.47, pp. 261-266, (2015). https://doi.org/10.9721/KJFST.2015.47.2.261
  23. D. H. Jin, H. S. Kim, J. H. Seong, H. S. Chung, "Comparison of Total Phenol, Flavonoid Contents, and Antioxidant Activities of Orostachys japonicus A. Berger Extracts", J. of Environmental Science International, Vol.25, No.5, pp. 695-703. (2016). https://doi.org/10.5322/JESI.2016.25.5.695
  24. D. H. Jin, J. M. Shin, J. H. Seong, Y. G. Lee, D. S. Kim, H. S. Chung, S. H. Jang, H. S. Kim, "Comparison of the Antioxidant Activities and Nitrite Scavenging Activity of Black Chokeberry (Aronia melanocarpa) Extracts", J. of Environmental Science International, Vol.25, No.4, pp. 567-578, (2016). https://doi.org/10.5322/JESI.2016.25.4.567
  25. E. Y. Kim, J. Y. Choi, M. Yu, M. Y. Kim, S. Lee, B. H. Lee, "Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants", Korean J. Food Sci. Technol., Vol.44, No.3, pp. 337-342, (2012). https://doi.org/10.9721/KJFST.2012.44.3.337
  26. N. J. Sung, "Physicochemical component and antioxidant activity of black garlic", Food Preservation and processing industry, Vol.7, No.1, pp. 45-53, (2008).
  27. The Korean Society of Food and Nutrition, Food Nutrition Dictionary, Korea Dictionary Research Publishing, p.671, (2004).

Cited by

  1. Lactobacillus plantarum에 의한 흰 민들레 발효물의 항산화 및 광노화 억제 효과 vol.22, pp.4, 2020, https://doi.org/10.5762/kais.2021.22.4.554