Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and immunoregulatory effects of Korean Rhamnaceae

국내 자생 갈매나무과 식물의 항산화 및 면역반응조절 효과 탐색

  • Eo, Hyun Ji (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kim, Da Som (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kang, Yeongyeong (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kim, Kiyoon (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Park, Youngki (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 어현지 (국립산림과학원 산림약용자원연구소) ;
  • 김다솜 (국립산림과학원 산림약용자원연구소) ;
  • 강연경 (국립산림과학원 산림약용자원연구소) ;
  • 김기윤 (국립산림과학원 산림약용자원연구소) ;
  • 박영기 (국립산림과학원 산림약용자원연구소) ;
  • 박광훈 (국립산림과학원 산림약용자원연구소)
  • Received : 2020.06.10
  • Accepted : 2020.07.01
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

We studied the antioxidative and immunoregulatory properties of 70% ethanol extracts from the leaves, branches, and fruit of 13 species of the Rhamnaceae collected during the bearing season (Berchemia berchemiifolia, Berchemia floribunda, Hovenia dulcis, Paliurus ramosissimus, Rhamnella franguloides, Rhamnus crenata, Rhamnus davurica, Rhamnus koraiensis, Rhamnus parvifolia, Rhamnus ussuriensis, Rhamnus yoshinoi, Sageretia thea, and Ziziphus ujube). Total phenolic and flavonoid content values were determined using calibration curves of gallic acid and rutin, respectively. ABTS and DPPH radical scavenging assays were performed to determine antioxidative potential. Anti-inflammatory effects were studied using the nitric oxide (NO) inhibition assay in LPS-induced RAW264.7 cells. Extracts of Berchemia berchemiifolia, Sageretia thea, and Ziziphus jujuba had the highest total phenol and flavonoid content values. Extracts of Berchemia berchemiifolia, Paliurus ramosissimus, Rhamnella franguloides, and Sageretia thea had the highest ABTS radical scavenging activities. Berchemia berchemiifolia and Sageretia thea had the highest DPPH radical scavenging activities. Furthermore, extracts of Berchemia berchemiifolia, Berchemia floribunda, Paliurus ramosissimus, Sageretia thea, and Ziziphus jujuba showed strong anti-inflammatory activity through inhibition of NO production. The results of this study suggest that Rhamnaceae extracts can be used as natural antioxidants and immunomodulators.

본 연구에서는 국내 자생하고 있는 갈매나무과 식물 13종(망개나무, 먹넌출, 헛개나무, 갯대추나무, 까마귀베개, 산황나무, 갈매나무, 털갈매나무, 돌갈매나무, 참갈매나무, 짝자래나무, 상동나무, 묏대추나무)에 대한 결실기 부위별(잎, 가지, 열매) 39점 70% ethanol 추출물의 총 페놀 및 플라보노이드 함량을 측정하고, ABTS 라디칼 소거능과 DPPH 전자 공여능 이용한 항산화 활성 및 NO 생성 억제력을 통한 항염증 활성을 측정하여 기능성 검토하였다. 총 페놀 및 플라보노이드 함량은 망개나무, 상동나무, 묏대추나무 추출물에서 높게 나타났다. 망개나무, 갯대추나무, 까마귀베개, 상동나무 추출물에서 높은 ABTS 라디칼 소거능을 보였고, 망개나무, 상동나무 추출물에서 약 80% 이상의 DPPH 전자공여능을 보였다. 또한, 망개나무, 먹넌출, 갯대추나무, 상동나무, 묏대추나무 등에서 50% 이상의 NO 생성 억제력을 보였다. 이러한 결과를 통하여 갈매나무과 식물의 이용 부위별 다양한 기능성을 검토하였고, 천연 항산화제 및 NO생성 억제를 통한 과도한 면역반응 완화 소재 개발 가능성을 확인하였다.

Keywords

References

  1. Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181(4617):1199-1200 https://doi.org/10.1038/1811199a0
  2. Chang CS and Kim H (2001) The distribution of the woody plants of South Korea based on herbarium (SNUA) material of the arboretum (VII) -Rhamnaceae-. Bull of Seoul Nat'l Univ Arboretum 21:1-15
  3. Choi DH, Cho UM, Hwang HS (2018) Anti-inflammation effect of rebaudioside A by inhibition of the MAPK and $NF-{\kappa}B$ signal pathway in RAW264.7 macrophage. J Appl Biol Chem 61(2):205-211 https://doi.org/10.3839/jabc.2018.030
  4. Choo GC, Kim SI, Chung Y, and Lee S (1993) A palynotaxonomic study of the Korean Rhamnaceae. Kor J Plnat Tax 23(3): 175-188 https://doi.org/10.11110/kjpt.1993.23.3.175
  5. Han SH, Woo NRY, Lee SD, Kang MH (2006) Antioxidative and antibacterial activities of endemic plants extracts in Korea. Korean J Medicinal Crop Sci 14(1):49-55
  6. Kim GS, Park CG, Lee KH, Choi J, Lee SE, Noh HJ, Lee JH, and Kim SY (2011) Investigation of Shikonin Pigments and antioxidant activity of the roots from Lithospermum erythrorhizon according to the different growth stages and areas of cultivation. Korean J Medicinal Crop Sci 19(6):435-440 https://doi.org/10.7783/KJMCS.2011.19.6.435
  7. Kim MJ, Kim KBYR, Jeong DH, Ahn DH (2013) Inhibitory effect of Sargassum fulvellum ethanolic extract on LPS-induced inflammatory reaction in RAW 264.7 mouse macrophages. J Appl Biol Chem 56(4):249-255 https://doi.org/10.3839/jabc.2013.040
  8. Kim KH, Ko KI, Kang EJ, Yang EK, Park SN (2004) A research trend of natural product on well-being industry. J Soc Cosmet Scientists Korea 30(3): 239-343
  9. Lee SG, Jo DJ, Chnag HJ, Kang H (2015) Antioxidant and anti-inflammatory activities of ethanol extracts from Aralia continentalis Kitagawa. Journal of Naturopathy 4(1&2):10-14
  10. Lee SO, Lee HJ, Yu MH, Im HG and Lee IS (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Kor J Food Sci Technol 37(2):233-240
  11. Park CH, Hur JM, Song KS and Park JC (2007) Phenolic compounds from the leaves of Nelumbo nucifera Showing DPPH radical scavenging effect. Kor J Pharmacogn 38(3): 263-269
  12. Park SB, Song HM, Kim HN, Park GH, Son HJ, Um Y, Park JA, Jeong JB (2018) Anti-inflammatory effect of biji (Soybean curd residue) on LPS-stimulated RAW264.7 cells. Korean J Plant Res 31(2):117-123 https://doi.org/10.7732/KJPR.2018.31.2.117
  13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, RiceEvans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9-10):1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  14. Richardson JE, Fay MF, Cronk QCB, Bowman D, Chase MW (2000) A phylogenetic analysis of Rhamnaceae using rbcL and trnL-F plastid DNA sequences. American Journal of Botany 87(9):1309-24 https://doi.org/10.2307/2656724
  15. Singleton VL, Orthofer R, and Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299C(1):152-178
  16. Song WY, Byeon SJ and Choi JH (2015) Anti-oxidative and anti-inflammatory activities of Sasa borealis extracts. Journal of Agriculture & Life Sciences 49(3):145-154
  17. Song WY, Choi JH (2017) Total phenols, flavonoid contents, and antioxidant activity of Spirodela polyrhiza extracts. Journal of Life Science 27(2):180-186 https://doi.org/10.5352/JLS.2017.27.2.180
  18. Woisky RG, Salatino A (1998) Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res 37(2):99-105 https://doi.org/10.1080/00218839.1998.11100961
  19. Woo HS, Lee SM, Heo JD, Lee MS, Kim YS and Kim DW (2018) Anti-inflammatory activity of extracts of Hovenia dulcis on lipopolysaccharides-stimulated RAW264.7 cells. Korean J Plant Res 31(5):466-477 https://doi.org/10.7732/KJPR.2018.31.5.466
  20. Yoo SJ and Kwak JH (1989) Phytochemical screening of Korean plants (I) on Urticaceae, Celastraceae, Rhamnaceae, Sterculiaceae and Rubiaceae. Kor J Pharmacogn 20(3):149-153
  21. Yu M, Im HG, Lee HJ, Ji YJ, and Lee IS (2006) Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis Rehder. Kor J Food Sci Technol 38:128-134