Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Protective Effect of water extract Phellinus linteus-discard Schisandra chinensis solid fermented extracts on improvement of sarcopenia by Atorvastatin-induced muscle atrophy cell model

Atorvastatin으로 유도된 근위축 세포모델에서 상황-오미자박 고상발효물 열수추출물의 보호효과

  • Kim, Young-Suk (Glucan Co. Ltd.) ;
  • Hwang, Su-Jin (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Park, Kwang-Il (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lim, Jong-Min (Glucan Co. Ltd.) ;
  • Cheon, Da-Mi (Glucan Co. Ltd.) ;
  • Jung, Yu Jin (Research Center for Advanced Specialty Chemicals, Korea Research Institute of Chemical Technology (KRICT)) ;
  • Jeon, Byeong Yeob (QBM Co,Ltd.) ;
  • Kwak, Kyeung Tae (QBM Co,Ltd.) ;
  • Oh, Tae Woo (Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM))
  • Received : 2021.11.18
  • Accepted : 2021.11.25
  • Published : 2021.11.30
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Abstract

Objectives : This study is to effect of improving muscle atrophy through water extract on the solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis in an atorvastatin-induced atrophy C2C12 cell. Methods : C2C12 myoblast were differentiated into myotube by 2% horse serum medium for 6 days, and then treated solid-phase fermentation(S-P) extract at different concentrations for 24h. To investigate the effect of S-P extract on the induction of muscle atrophy and expression of atrophy-related genes and apoptosis in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis. Results : As a result of treatment with atorvastatin at concentrations of 5, 10, and 20 uM on the 6th day of differentiation in C2C12 myotube cells, it was confirmed that the cell morphology was damaged in a concentration-dependent manner, and the length and thickness of the myotube also decreased in a concentration-dependent manner. Treatment with S-P extract (50, 100 and 200 ㎍/㎖) increased of GSH and inhibited ROS in the atorvastatin-induced muscle atrophy cell model at a concentration that did not induce toxicity. In addition, it was confirmed that it has an effect on muscle reduction by inhibiting apoptosis of muscle cells as well as being involved in protein production and degradation of muscle cells. Conclusions : Atorvastatin-induced atrophy C2C12 cell, S-P extract activates related to differentiation/generation and proteolysis, and inhibits cell death of atrophy in C2C12 cell. Based on this, it is necessary to prove its effectiveness through animal models and human application test, but it is considered to be discarded Schisandra chinensis can present the potential for development as a recycling industrial material.

Keywords

Acknowledgement

본 결과물은 환경부의 재원으로 한국환경산업기술원의 야생생물 유래 친환경 신소재 및 공정기술개발사업의 지원을 받아 연구되었습니다. (RE202101318, 한국한의학연구원 ERT2111080).

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