The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Comparative Proteomic Analysis for a Putative Pyridoxal Phosphate-Dependent Aminotransferase Required for Virulence in Acidovorax citrulli

  • Lee, Jongchan (Department of Plant Science and Technology, Chung-Ang University) ;
  • Heo, Lynn (Department of Plant Science and Technology, Chung-Ang University) ;
  • Han, Sang-Wook (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2021.09.09
  • Accepted : 2021.10.13
  • Published : 2021.12.01
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Abstract

Acidovorax citrulli (Ac) is the causative agent of bacterial fruit blotch disease in watermelon. Since resistant cultivars have not yet been developed, the virulence factors/mechanisms of Ac need to be characterized. This study reports the functions of a putative pyridoxal phosphate-dependent aminotransferase (PpdaAc) that transfers amino groups to its substrates and uses pyridoxal phosphate as a coenzyme. It was observed that a ppdaAc knockout mutant had a significantly reduced virulence in watermelon when introduced via germinated-seed inoculation as well as leaf infiltration. Comparative proteomic analysis predicted the cellular mechanisms related to PpdaAc. Apart from causing virulence, the PpdaAc may have significant roles in energy production, cell membrane, motility, chemotaxis, post-translational modifications, and iron-related mechanisms. Therefore, it is postulated that PpdaAc may possess pleiotropic effects. These results provide new insights into the functions of a previously unidentified PpdaAc in Ac.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. NRF-2020R1A2C1013040). This research was supported by the Chung-Ang University research grant in 2021. (to S.W. Han).

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