Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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The antioxidant roles of L-carnitine and N-acetyl cysteine against oxidative stress on human sperm functional parameters during vitrification

  • Ghorbani, Fatemeh (Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences) ;
  • Nasiri, Zohreh (Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences) ;
  • Koohestanidehaghi, Yeganeh (Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences) ;
  • Lorian, Keivan (Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences)
  • Received : 2021.04.22
  • Accepted : 2021.06.23
  • Published : 2021.12.31
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Abstract

Objective: Amino acids can protect sperm structure in cryopreservation due to their antioxidant properties. Therefore, the present study aimed to investigate the protective effect of L-carnitine (LC) and N-acetyl cysteine (NAC) on motility parameters, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), DNA damage, and human sperm intracellular reactive oxygen species (ROS) during vitrification. Methods: Twenty normal human sperm samples were examined. Each sample was divided into six equal groups: LC (1 and 10 mM), NAC (5 and 10 mM), and cryopreserved and fresh control groups. Results: The groups treated with LC and NAC showed favorable findings in terms of motility parameters, DNA damage, and MMP. Significantly higher levels of intracellular ROS were observed in all cryopreserved groups than in the fresh group (p≤0.05). The presence of LC and NAC at both concentrations caused an increase in PMI, MMP, and progressive motility parameters, as well as a significant reduction in intracellular ROS compared to the control group (p≤0.05). The concentrations of the amino acids did not show any significant effect. Conclusion: LC and NAC are promising as potential additives in sperm cryopreservation.

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References

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