Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of water extract of Paeoniae Radix Alba on a thioacetamide induced acute liver injury rat model

Thioacetamide 유발 급성 간손상 동물모델에 백작약 열수 추출물이 미치는 효능

  • Lee, Se Hui (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ji Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이세희 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이지혜 (한국한의학연구원 한약자원연구센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2021.01.18
  • Accepted : 2021.02.18
  • Published : 2021.04.30
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Abstract

Purpose: Paeonia Radix Alba is a traditional herbal medicine used to treat the liver and the spleen. Many studies have reported that Paeonia Radix Alba extract (PR) affects liver injury, but there has been no study on liver injuries induced by thioacetamide (TAA). Therefore, we aimed at evaluating the effect of PR on a TAA-induced acute liver injury (ALI) model. Methods: The antioxidant activity of PR was assayed by the content of total polyphenol, total flavonoid, 1,1-diphenyl-2'-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) radical scavenging activities in vitro test. ALI was induced via-intraperitoneal injection of TAA (200 mg/kg body weight) for three consecutive days. Also, silymarin (100 mg/kg body weight) and PR (100 or 200 mg/kg body weight) were administered at 1 hours 30 minutes prior to TAA treatment. The levels of ammonia, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) were analyzed using an assay kit. The expressions of antioxidant proteins including Nrf2, Keap1, HO-1, SOD, catalase, and GPx-1/2 and oxidative stress-related proteins including NOX2, p47phox, and p22phox were evaluated by the western blot analysis. Results: PR showed excellent antioxidant activity in vitro. TAA administration increased the levels of ammonia, GOT, and GPT in the ALI control group compared to the normal group, whereas it was significantly reduced by PR pretreatment. Moreover, NADPH oxidase protein expressions were upregulated after TAA treatment, while the elevated expressions were inhibited by PR pretreatment. The expressions of antioxidant protein were downregulated in the ALI control group, whereas Nrf2 activation in the PR group was accompanied by increased levels of antioxidant enzymes. Conclusion: PR administration increased the antioxidant enzymes via activation of the Keap1/Nrf2 pathway and inhibited the protein levels of NADPH oxidase factors. Taken together, these results showed that PR treatment may be considered to ameliorate acute liver injury induced by TAA.

본 연구는 백작약 열수 추출물의 in vitro 항산화능을 평가하였으며, TAA를 유발한 급성 간손상 동물 모델을 이용하여 항산화 활성으로 인한 산화적 스트레스 억제 효과와 간 기능 보호효과 여부를 검증하였다. 총 폴리페놀, 총 플라보노이드 함량, DPPH 및 ABTS 자유 라디칼 소거능 측정 결과 높은 항산화능을 나타냈으며, 체중, 간무게 및 간중량 비율 (%)이 대조군에 비해 S100군과 PR100군보다 PR200군에서 더욱 감소하였다. 약물투여군의 혈중 암모니아, GOT 및 GPT 수준의 유의적인 감소를 확인하였으며, western blot 실시 후 대부분의 인자에서 유의적인 차이를 확인하였다. 이 결과를 토대로 백작약 열수 추출물이 Nrf2-Keap1경로의 활성화를 통해 항산화 효소를 증가시켰으며, 항산화능을 통한 산화적 스트레스 개선에 긍정적인 효과가 있음을 확인할 수 있었다. 따라서 백작약 열수 추출물은 급성 간손상시 간보호 효과를 위한 후보 소재로서 가능성이 있다고 판단된다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2018R1A5A2025272).

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