Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of molecular markers for the differentiation of Angelica gigas Jiri line by using ARMS-PCR analysis

세발당귀(Angelica gigas Jiri)의 판별을 위한 ARMS-PCR용 분자표지 개발

  • Lee, Shin-Woo (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Lee, Soo Jin (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Han, Eun-Hee (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Shin, Yong-Wook (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Kim, Yun-Hee (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University)
  • 이신우 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 이수진 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 한은희 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 신용욱 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 김윤희 (경상국립대학교 생명과학대학 항노화신소재과학과)
  • Received : 2021.02.04
  • Accepted : 2021.03.23
  • Published : 2021.03.31
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Abstract

Angelica is a widely used medicinal and perennial plant. Information on the genetic diversity of Angelica populations is essential for their conservation and germ plasmic utilization. Although Angelica is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish it from other similar species from different countries. This developed single nucleotide polymorphism (SNP) markers derived from nuclear ribosomal DNA internal transcribed spacer regions genomic sequences to identify distinct Korean-specific Angelica species via amplification refractory mutation system (ARMS)-PCR curve analyses. We performed molecular authentication of different kinds of Korean-specific Angelica species such as A. gigas Nakai and A. gigas Jiri using DNA sequences in the ITS intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific Angelica species from different countr.

당귀는 일반적으로 이용되는 대표적인 다년생의 약용식물이다. 최근 국제적 추세에 따라 자국의 유전자원의 발굴, 보존 등이 강화됨에 따라 인접국가와 국내 자생 당귀 계통을 판별할 수 있는 기준설정에 관한 연구의 필요성이 대두되고 있지만, 분자생물학적 판별 기술의 개발은 아직 미흡한 실정이다. 본 연구에서는 국내 토종 당귀인 참당귀와 세발당귀, 그리고 해외 유래 당귀 종의 기원을 판별하기 위해 핵의 리보솜에 존재하는 ITS 유전자단편에서 SNP를 이용한 판별 프라이머를 확보하였으며, 이를 보완하여 보다 신속하게 판별하기 위하여 ARMS-PCR 기술을 이용한 판별 마커와 그 조건을 확립하였다. 그러므로, 본 연구에서 개발된 SNP 마커는 다양한 지역 또는 국가에서 서식하는 당귀 종들의 신속한 확인을 위해 매우 유용하게 이용될 것으로 생각된다.

Keywords

Acknowledgement

이 논문은 2020~2021년도 경남과학기술대학교 대학회계 연구비 지원에 의하여 연구되었음.

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