Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Biosynthesis of Compound K, a biologically active saponin of ginseng(Panax ginseng) by bioconversion

인삼(Panax ginseng)으로부터 생물전환을 이용한 생리활성물질인 Compound K의 생합성

  • Kim, MooSung (Macrocare Co., Ltd) ;
  • Kim, Ja-i (Division of Food Science and Biotechnology, Korea National University of Transportation) ;
  • Jung, Kyung-Hwan (Division of Food Science and Biotechnology, Korea National University of Transportation) ;
  • Yu, Kwang-Won (Division of Food Science and Biotechnology, Korea National University of Transportation) ;
  • Moon, Gi-Seong (Division of Food Science and Biotechnology, Korea National University of Transportation) ;
  • Lee, Hyang-Yeol (Division of Food Science and Biotechnology, Korea National University of Transportation)
  • 김무성 (마크로케어) ;
  • 김자이 (한국교통대학교 식품생명학부) ;
  • 정경환 (한국교통대학교 식품생명학부) ;
  • 유광원 (한국교통대학교 식품생명학부) ;
  • 문기성 (한국교통대학교 식품생명학부) ;
  • 이향렬 (한국교통대학교 식품생명학부)
  • Received : 2021.10.11
  • Accepted : 2021.10.29
  • Published : 2021.10.30
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Abstract

Ginsenoside Compound K is a triterpene saponin found in the leafs, stems and roots of Panax ginseng. This study aimed to prepare a valuable ginsenoside Compound K using ginseng extracts with the enzyme(Plantase). Plantase showed very efficient activity to produce Compound K from ginseng extracts. Plantase exhibited the highest activity at pH 5 and 50 ℃, as a result of investigating the yield of Compound K by changing the temperature and pH, while fixing the enzyme concentration to 10% or 15% over 48 hours of reaction time. Under optimium conditions, Plantase produced and accumulated Compound K over 35 wt% of whole ginseng extracts. Antimicrobial activitiy of bioconvertied ginseng extracts showed selectivity against Cutibacterium acnes KCTC 3314. Minimal inhibitory concentration (MIC) of bioconverted ginseng extract (35% of Compound K enriched extract) against Cutibacterium acnes KCTC 3314 strain is 31.25ug/mL. These results suggest that the Compound K enriched extract is potential materials for cosmetic products and Plantase is a very useful enzyme for Compound K production.

진세노사이드 Compound K는 트라이터펜계 사포닌으로써 인삼의 잎, 줄기, 뿌리등에서 발견된다. 본 연구는 효소 Plantase를 이용하여 인삼 추출물로부터 고부가가치의 진세노사이드인 Compound K를 생산하는 연구를 하였다. Plantase는 인삼추출물에서 Compound K를 매우 효율적으로 생산함을 보여 주었다. 또한 다양한 온도와 pH에서 Compound K 생산에 대한 최적의 반응을 조사한 결과 pH 5, 50 ℃에서 가장 높은 효율을 보였다. 최적 조건에서 Compound K는 전체 추출물의 35%이상 농축될 수 있음을 확인하였다. 생물전환된 Compound K 농축물의 항균효과를 검정한 결과 여드름균인 Cutibacterium acnes KCTC 3314에 선택적인 활성을 보였다. Compound K (35% 함유) 인삼 생물전환물의 C. acnes KCTC 3314 균주에 대한 최소저해농도 측정 결과 31.25ug/mL로 확인되었다. 따라서 향후 여드름균 완화용 화장품의 잠재적 소재로 사용될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

이 논문은 2021년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구지원사업(No. 2021R1A6A1A03046418)이며, 또한 2021년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다(No. 2021RIS039301).

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