Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines

  • Vu, Thi Hao (Department of Animal Science and Technology, Chung-Ang University) ;
  • Hong, Yeojin (Department of Animal Science and Technology, Chung-Ang University) ;
  • Truong, Anh Duc (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Lee, Jiae (Department of Animal Science and Technology, Chung-Ang University) ;
  • Lee, Sooyeon (Department of Animal Science and Technology, Chung-Ang University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, College of Agricultural and Life Sciences, Jeonbuk National University) ;
  • Cha, Jihye (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Dang, Hoang Vu (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Tran, Ha Thi Thanh (Department of Biochemistry and Immunology, National Institute of Veterinary Research) ;
  • Lillehoj, Hyun S. (Animal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture) ;
  • Hong, Yeong Ho (Department of Animal Science and Technology, Chung-Ang University)
  • Received : 2021.04.08
  • Accepted : 2021.06.07
  • Published : 2022.03.01
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Abstract

Objective: The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well as the economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for HPAIV resistance. Therefore, in this study, we investigated gene expression related to cytokine-cytokine receptor interactions by comparing resistant and susceptible Ri chicken lines for avian influenza virus infection. Methods: Ri chickens of resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) lines were selected by genotyping the Mx dynamin like GTPase (Mx) and major histocompatibility complex class I antigen BF2 genes. These chickens were then infected with influenza A virus subtype H5N1, and their lung tissues were collected for RNA sequencing. Results: In total, 972 differentially expressed genes (DEGs) were observed between resistant and susceptible Ri chickens, according to the gene ontology and Kyoto encyclopedia of genes and genomes pathways. In particular, DEGs associated with cytokine-cytokine receptor interactions were most abundant. The expression levels of cytokines (interleukin-1β [IL-1β], IL-6, IL-8, and IL-18), chemokines (C-C Motif chemokine ligand 4 [CCL4] and CCL17), interferons (IFN-γ), and IFN-stimulated genes (Mx1, CCL19, 2'-5'-oligoadenylate synthase-like, and protein kinase R) were higher in H5N1-resistant chickens than in H5N1-susceptible chickens. Conclusion: Resistant chickens show stronger immune responses and antiviral activity (cytokines, chemokines, and IFN-stimulated genes) than those of susceptible chickens against HPAIV infection.

Keywords

Acknowledgement

We thank Department of Biochemistry and Immunology in the National Institute of Veterinary Research, Vietnam for performing animal experiments and collecting samples.

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