The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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Synergistic interaction between acetaminophen and L-carnosine improved neuropathic pain via NF-κB pathway and antioxidant properties in chronic constriction injury model

  • Owoyele, Bamidele Victor (Neuroscience and Inflammation Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin) ;
  • Bakare, Ahmed Olalekan (Neuroscience and Inflammation Unit, Department of Physiology, Adeleke University) ;
  • Olaseinde, Olutayo Folajimi (Neuroscience and Inflammation Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin) ;
  • Ochu, Mohammed Jelil (Neuroscience and Inflammation Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin) ;
  • Yusuff, Akorede Munirdeen (Neuroscience and Inflammation Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin) ;
  • Ekebafe, Favour (Neuroscience and Inflammation Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin) ;
  • Fogabi, Oluwadamilare Lanre (Neuroscience and Inflammation Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ilorin) ;
  • Roi, Treister (Department of Nursing, Faculty of Social Welfare and Health Sciences, University of Haifa)
  • Received : 2022.02.03
  • Accepted : 2022.04.18
  • Published : 2022.07.01
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Abstract

Background: Inflammation is known to underlie the pathogenesis in neuropathic pain. This study investigated the anti-inflammatory and neuroprotective mechanisms involved in antinociceptive effects of co-administration of acetaminophen and L-carnosine in chronic constriction injury (CCI)-induced peripheral neuropathy in male Wistar rats. Methods: Fifty-six male Wistar rats were randomly divided into seven experimental groups (n = 8) treated with normal saline/acetaminophen/acetaminophen + L-carnosine. CCI was used to induce neuropathic pain in rats. Hyperalgesia and allodynia were assessed using hotplate and von Frey tests, respectively. Investigation of spinal proinflammatory cytokines and antioxidant system were carried out after twenty-one days of treatment. Results: The results showed that the co-administration of acetaminophen and L-carnosine significantly (P < 0.001) increased the paw withdrawal threshold to thermal and mechanical stimuli in ligated rats compared to the ligated naïve group. There was a significant (P < 0.001) decrease in the levels of nuclear factor kappa light chain enhancer B cell inhibitor, calcium ion, interleukin-1-beta, and tumour necrotic factor-alpha in the spinal cord of the group coadministered with acetaminophen and L-carnosine compared to the ligated control group. Co-administration with acetaminophen and L-carnosine increased the antioxidant enzymatic activities and reduced the lipid peroxidation in the spinal cord. Conclusions: Co-administration of acetaminophen and L-carnosine has anti-inflammatory effects as a mechanism that mediate its antinociceptive effects in CCI-induced peripheral neuropathy in Wistar rat.

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