Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Structural Analysis of Antitumor Polysaccharides Obtained from Ganoderma lucidum IY 009

Ganoderma lucidum IY 009로 부터 분리된 항암성 다당류의 정제 및 구조분석

  • Lee, Kweon-Haeng (Department of Food Engineering, College of Engineering, Yonsei University. Bioproducts Research Center) ;
  • Jeong, Hoon (Biotechnology Lab., Central Institute of Research, Il Yang Pharmaceutical Co., Ltd.) ;
  • Lee, June-Woo (Biotechnology Lab., Central Institute of Research, Il Yang Pharmaceutical Co., Ltd.) ;
  • Han, Man-Deuk (Biotechnology Lab., Central Institute of Research, Il Yang Pharmaceutical Co., Ltd.) ;
  • Choi, Kyoung-Sook (Engineering Research Institute, Yonsei University) ;
  • Oh, Doo-Hwan (Department of Food Engineering, College of Engineering, Yonsei University. Bioproducts Research Center)
  • 이권행 (연세대학교 식품생물공학과 생물산업소재연구센터) ;
  • 정훈 (일양약품(주) 중앙연구소 생물공학연구실) ;
  • 이준우 (일양약품(주) 중앙연구소 생물공학연구실) ;
  • 한만덕 (일양약품(주) 중앙연구소 생물공학연구실) ;
  • 최경숙 (연세대학교 산업기술연구소) ;
  • 오두환 (연세대학교 식품생물공학과 생물산업소재연구센터)
  • Published : 1994.04.01
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Abstract

Alkali soluble(AS) fraction, revealed the highest antitumor activity of the alkali extracted fractions of G. lucidum IY 009, was loaded on DEAE cellulose(OH$^{-}$ form) column. AS-1, AS-2, AS-3, AS-4 and AS-5 were obtained by stepwise elution with H$_{2}$O, 0.1 M NaHCO$_{3}$, 0.3 M NaHCO$_{3}$, 0.5 M NaHCO$_{3}$ and 0.5 N NaOH respectively, and their antitumor activities(I.R. %) against the sarcoma 180 were 97.5%, 68.0%, 73.0%, 81.0% and 66.0% respectively. AS-1 observed highest antitumor activity was appeared as single peak on the Sepharose CL-4B column chromatography, and their molecular weight was about 580,000 dalton. The carbohydrate content of AS-1 was 98.9%, their monosaccharide consisted of 67.5% of mannose, 22.5% of xylose, 5.8% of glucose, 1.8% of galactose and 2.0% of ribose. AS-1 was assumed $\alpha $linkaged xylomannan having infrared absorption at 864.3 cm$^{-1}$. The main alditol acetates of AS-1 were identified as 1,5-Di-O-acetyl1-2,3,4-Tri-O-methylxylitol, 1,4,5-Tro-O-acety1-2,3,6-Tri-O-methylmannitol and 1,3,4,5-Tetra-O-acety1-2,6-Di-O-methylmannitol by methylation analysis, and their molar ratio was 1 : 2 : 1. The core portion of AS-1 might be $\alpha $-(1$\longrightarrow $ 4)mannopyranosyl unit branched with side chain, C1 of xylopyranosyl residue linked to C3 of every 3 mannopyranosyl units, and the degree of polymerization of structural unit in AS-1 was about 835.

Keywords

References

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