Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Antimutagenic and Antigenotoxic Effects of Ligularia fischeri Extracts

곰취 추출물의 항돌연변이성 및 유전독성억제효과

  • 함승시 (강원대학교 식품생명공학부) ;
  • 이상영 (강원대학교 식품생명공학부) ;
  • 오덕환 (강원대학교 식품생명공학부) ;
  • 정성원 (강원대학교 식품생명공학부) ;
  • 김상헌 (강원대학교 농기계공학과) ;
  • 정차권 (한림대학교 식품영양학과) ;
  • 강일준 (한림대학교 식품영양학과)
  • Published : 1998.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The antimutagenic and antigenotoxic effects of ethanol, methanol, water and non-heating ethanol extract of Ligularia fischeri were investigated using Ames test and micronucleus test. Four solvent extracts by themseleves did not induce mutagenesis. The four extract of 200㎍/plate showed approximately 84.7%, 77.1%, 72.5% and 71% inhibitory effect on the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 67.9%, 66.8%, 64.6% and 56% inhibition on the mutagenesis by 4-nitroquinoline-1-oxide(4NQO) against TA100 strain, whereas 70.2%, 60.9%, 61.9% and 52.8% inhibitions were observed on the mutagenesis induced by 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indol(Trp-P-1) in the presence of 200㎍/plate. TA100 strain was more sensitive than TA98 strain by four kinds of extracts on antimutagenesis. The effects of Ligularia fischeri extracts on the frequencies of micronucleated poly chromatic erythrocytes(MNPECs) induced by MNNG were investigated in the bone marrow. Ten, 20, 40 and 80mg g/kg of each extract were administered to animals immediately after injection of MNNG and the exposure time was 36 hours. Inhibitory effects of Ligularia fischeri ethanol extracts were 12%, 35.3%, 58.8%, and 57%, in the presence of 20, 40, 60 and 80mg/kg, respectively whereas methanol extracts showed 15.5%, 32.7%, 50.8%, and 57.9% inhibitory effects, respectively. Both extracts showed enhanced antimutagenic and antigenotoxic effects. These results showed a good correlation between antimutagenic effects in in vitro and in in vitro assay.

Keywords

References

  1. J. Korean. Soc. Food. Nutr. v.21 no.308 Antimutagenic effect of the major volatile compounds identified from Mugwort Kim, J. O.;Kim, Y. S.;Lee, J. H.;Kim, M. N.;Rhee, S. H.;Moon, S. H.;Park, K. Y.
  2. Mutation Res. v.53 no.351 Antimutagenic action of vegetabel factors on the mutagenic principle of tryptophane pyrolysate Tsuneo, K.;Kazuyoshi, M.;Tadashi, I.
  3. Mutation Res. v.141 no.149 Adsorption of pyrolysate mutagen by vegetable fibers Tsuneo, K.;Masayuki, K.;Katsuhiro, A.;Shuhach, K.
  4. Pro. Natl. Acad. Sci. v.89 no.2399 An anticarcinogenic protective enzyme from broccoli Zhang, Y.;Talay, P.;Cho, C. G.;Posner, G. H.
  5. J. Agric. food. Chem. v.37 no.1363 Isolation of compounds with antimutagenic activity from savory chieften cabbage Lason, T.;Nunnally, J.;Walker, B.;Bresnik, E.;Wheeler, D.;Wheeler, M.
  6. J. Korean. Soc. Food. Nutr. v.21 no.302 Antimutagenic compound identified from perilla leaf Lee, K. L.;Rhee, S. H.;Park, K. Y.;Kim, J. O.
  7. Inform v.4 no.344 Desinging manipulating foods to promote health Human, B. F.
  8. 보건사회 백서 보건사회부
  9. Antimutagenesis and anticarcinogenesis mechanism III Inhibitory effects of dietary leafy vegetables on mutagens and active oxygens Ebata, J.;Kawai, K.;Furukawa, H.;Bronzetti, G.(et al. eds.)
  10. Phenolic compounds in food and their effects on health II Antioxidants and cancer prevention Phenolic antioxidants in dietary plants as mutagen Osawa, T.;Huang, M. T.(eds);Ho, C. T.(eds);Lee, C. Y.(eds)
  11. J. Agric. Food. Chem. v.43 no.410 A strong antimutagen agains dietary carcinogen, Trp-P-2 in peppermint, sage and thyme Samejima, K.;Kanazawa, K.;Nanno, G.
  12. 식품성분표(제5개정판) 농촌진흥청 생활연구소
  13. 중국 장백산 천연약제 김학배;김정일
  14. Mutation. Res. v.113 no.179 Revised methods for the Salmonella typhimurium mutagenicity test Ames, B. N.;Maron, D. M.
  15. Mutation Res v.19 no.109 The micronucleus test, methodological aspects Ledebur, M. V.;Schmid, W.
  16. Mutation Res v.31 no.9 The micronucleus test Schmid, W.
  17. 한국식품위생학회지 v.7 no.16 Trp-P-1과 2-AF에 대한 산채류 생즙의 항돌연변이효과 한규석;함승시;정의호;이해금
  18. 한국식품위생학회지 v.8 no.225 2-AF에 의해 유발된 미생물 변이원성에 미치는 들미나리즙의 돌연변이 억제작용 한규석;정의호;함승시;심태흠;이택수;이해금
  19. 한국영양식량학회지 v.23 no.698 메밀 flavonoids의 항돌연변이원성 및 지질대사 조절기능에 관한 연구-메밀 잎 에탄올 추출물의 항돌연변이원성 연구- 함승시;최근표;최용순;이상영
  20. J. Food. Sci. Nutr. v.2 no.155 Antimutagenic effects of juices from edible Korena wild herbs Ham, S. S.;Oh, D. H.;Hong, J. K.;Lee, J. H.
  21. 한국식품영양과학회지 v.26 no.92 산채류를 이용한 음료 개발에 관한 연구 함승시;이상영;오덕환;김상헌;홍정기
  22. J. Food. Sci. Nutr. v.2 no.162 Antigenotoxic effects of Synurus deltoides extract on benzo[a]pyrene induced mutagenesis Ham,S. S.;Han, H. S.;Choi, K. P.;Oh, D. H.
  23. 한국식품영양과학회지 v.27 no.57 목이 및 석이 메탄올 추출물의 유전독성 억제효과 함승시;김득하;최근표;이득식