Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antitumor Activity of Paecilomyces japonica is Mediated by Apoptotic Cell Death

  • Park, Youn-Hee (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Moon, Eun-Kyung (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Shin, Yong-Kyu (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Bae, Myung-Ae (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Young-Ho (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
  • Published : 2000.02.01
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Abstract

The aqueous extract from the cultural mycelium of Paecilomyces japonica showed cytotoxicity against several tumor cells including Jurkat, U937, HL-60, HepG2, BW5147.G.1.4, and NIH3T3. When the aqueous extract was fractionated by sequential organic solvent extractions using n-hexane and ethyl acetate, the ethyl acetate fraction appeared to contain the most cytotoxic activity, and the $IC_{50}$ values for various tumor cells were in the range from 1.5 to $10.0{\;}\mu\textrm{g}/ml$. To elucidate the cellular mechanism underlying the induced cytotoxicity, the apoptotic DNA fragmentation along with the cell cycle proression was examined in Jurkat T cells following the ethyl acetate fraction treatment. In the presence of $2.5{\;}\mu\textrm{g}/ml$ of the ethyl acetate fraction, apoptotic DNA fragmentation of the cells was detected within 1 h and increased upto 24 h in a time-dependent manner. Under the same conditions, a sub-G1 peak was detectable by flow cytometry. These results indicate that the cytotoxic effect of P. japonica on tumor cells is attributable to the induced apoptosis.

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References

  1. Biophys. Res. Commun. v.246 Enhanced expression of Fas ligand is associated with aburatubolactam C-induced apoptosis in human Jurkat T cells. Biochem Bae, M.A.;H. S. Kang;S. W. Rue;J. H. Seu;Y. H. Kim
  2. J. Microbiol. Biotechnol. v.8 Aburatubolactam C, a novel apoptosis-inducing substance produced by marine Streptomyces sp. SCRC A-20 Bae, M. A.;K. Yamada;D. Uemura;J. H. Seu;Y. H. Kim
  3. Kor. J. Mycol. v.26 Cultural characteristics for inducing fruiting-body of Isaria japonica. Ban, K.-W;D.-K. Park;J.-O. Shim;Y.-S., Lee;C.-H. Park;J.-Y. Lee;T.-S. Lee;S.-S. Lee;M.-W. Lee
  4. Ascomycetes. Mykologia luzern v.1 Fungi in Switzerland. Breitenbach, J;F. Kranzlin
  5. Data for Biochemical Research(3rd edition) Dawson, R. M. C.;D. C. Elliott;W.H. Elliot;K.M. Jones
  6. Nucleic Acid Res. v.3 The sensitivity of RNA polymerases Ⅰand Ⅱ from Novikoffhepatoma(N1S1) cells to 3'-deoxyadenosine 5'-triphosphate. Desrosiers, R. C.;F. M. Rottman;J. A. Boezi;H.C. Towle
  7. Ecol. Entomol. v.7 Entomogenous fungi in tropical forest ecosystem: An appraisal Evans, H. C.
  8. Nucleic Acid Res. v.3 Identification of cordycepin a metabolite of Cordyceps militaris, as 3'-deoxyadenosine 5'-triphosphate. Kaczka, E. A.;N. R. Trenner;B. Arison;R. W. Walker;K. Folkers.
  9. J. Immunol. v.149 Expression of the murine homologue of the cell cycle control protein $p34^cdc2$ in T lymphocytes. Kim, Y. H.;J. J. Proust;M. J. Buchholz;F. J. Chrest;A. A. Nordin.
  10. Iconography of Vegetable Wasps and Plant Worms. Kovayasi, Y;D. Shimizu.
  11. Annu. Rev. Entomol. v.11 Fungal parasites of insects. Medelin, M. F.
  12. Fundermentals of the Fungi(4th ed) Moore-Landecker, E
  13. Kor. J. Mycol. v.25 Distribution and taxonomy of entomopathogenic fungal species from Korea. Sung, J.-M;H.-K. Lee;Y.-S. Choi;Y.-Y. Kim;S.-H. Kim;G.-H. Sung
  14. Kor. J. Mycol. v.23 Classification of Cordyceps spp. by morphological characteristics and protein banding pattern. Sung, J.-M.;H.-K. Lee;K.-J. Yang.