Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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New Antimicrobial Activity from Korean Radish Seeds (Raphanus sativus L.)

  • Park, Jong-Heum (Institute of Functional Food and Safety, Handong University) ;
  • Shin, Keuyn-Kil (School of Bioscience and Food Technology, Handong University) ;
  • Hwang, Cher-Won (School of Bioscience and Food Technology, Handong University)
  • Published : 2001.04.01
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Abstract

To isolate antifungal substances from Korean radish (Raphanus Sativus L.) seeds, various purification techniques such as DE52 cellulose anion exchange, SP-Sephadex C-25 cation exchange, and Sephadex G-50 gel filtration chromatographies were used. The molecular masses of two purified R. sativus antifungal proteins (RAPs) were estimated to be about 6.1 kDa (RAP-1) and 6.2 kDa (RAP-2) by SDS-PAGE, and 5.8 kDa(RAP-1) and 6.2 kDa (RAP-2 by a gel filtration chromatography, respectively. Purified proteins RAP-1 and 2 clearly exhibited different growth inhibitory activities against other microorganisms like Candida albicans and Saccharomyces cerevisiae. Although they have similar molecular masses, both RAP-1 and 2 proteins are not identical because their microbial inhibitory actions were different. Therefore, RAP-1 could be a new antifungal protein when compared with the antifungal activities of 2S albumins, Rs-AFPs, Mj-AMPs, chitinase, glucanase, permatin, and ribosome inactivating proteins, all of which are anifungal proteins of plants.

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References

  1. J. Microbiol. Biotechnol. v.10 Isolation of bacterial strain antagonistic to Pyricularia oryzae and its mode of antifungal action Bae, D. W.;J. T. Lee;D. Y. Son;E. S. Lee;H. K. Kim
  2. J. Microbiol. Biotechnol. v.10 Puriffication and characterization of a novel antifungal protein from Paenibacillus macerans PMI antagonistic to rice blast fungus, Pyricularia oryzae Bae, D. W.;Y. S. Kawk;J. T. Lee;D. Y. Son;S. S. Chun;H. K. Kim
  3. EMBO J. v.7 Leaf-specific thionins of barley - a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants Bohlmann, H.;S. Clausen;S. Behnke;H. Giese;H. Hiller;U. Reimann-Philipp;G. Scharder;V. Barkholt;K. Apel
  4. Biochemistry v.31 Antimicrobial peptides from Amaranthus cauatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins Broekaert, W. F.;W. Marien;F. R. Terras;M. F. De Bolle;P. Proost;J. Van Damme;L. Dillen;M. Claeys;S. B. Rees;J. Vanderleyden;B. P. Cammue
  5. J. Biol. Chem. v.267 Isolation and characterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds Cammue, B. P.;M. F. De Bolle;F. R. Terras;P. Proost;J. Van Damme;S. B. Rees;J. Vanderleyden;W. F. Broekaert
  6. J. Microbiol. Biotechnol. v.9 Purification and structure determination of antifungal phospholipids from a marine Streptomyces Cho, K. W.;Y. W. Seo;T. M. Yoon;J. H. Shin
  7. J. Mol. Biol. v.279 Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by ¹H NMR Fant, F.;W. Vranken;W. Broekaert;F. Borremans
  8. Appl. Microbiol. v.23 Susceptibility of phytopathogenic bacteria to whcat purothionins in vitro Fernandez de Claeya, R.;B. Gonzaley-Pascular;F. Garcia-Olmedo;P. Carbonero
  9. J. Micro. Biotechnol. v.7 A partial nucleotide sequence of chitin synthase(CHS) gene from rice blast fungus, Pyricularia oryzae and its cloning Hwang, C. W.;I. C. Park;W. H. Yea;M. Takagi;J. C. Ryu
  10. J. Microbiol. Biotechnol. v.8 Molecular cloning and sequence of URA5 gene encoding-orotate phosphoribosy1 transferase (OPRTase) from Entomopathogenic fungus, Metarhizium anisopliae Hwang, C. W.;D. K. Lee;S. C. Kang
  11. J. Microbiol. Biotechnol. v.10 Antimicrobial effectiveness of pine needle extract on food borne illness bacteria Kim, K. Y.;M. Davidson;H. J. Chung
  12. J. Biol. Chem. v.266 biochemical and molecular characterization of three barley seed proteins with antifungal properties Leah, R.;H. Tommerup;I. Svendsen;J. Mundy
  13. Phytochemistry v.12 Some properties of a 1,3-Glucanase from RYE Manners, D. J.;J. J. Marshall
  14. FEBS Lett. v.368 Isolation and characterization of plant defensin from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae Osborn, R. W.;G. W. De Samblanx;K. Thevisson;I. Goderis;S. Torrekens;F. Van Leuven;S. Attenborough;S. B. Rees;W. F. Broekaert
  15. Anticancer Drug v.10 An antimelanorma-barley ribosome inactivation protein conjugate is cytotoxic to melanoma cells in vitro Ovalia, M.;C. C. Hager;T. N. Oeltmann
  16. Biochim. Biophys. Acta v.880 Isolation and partial characterization of two antifungal proteins from barley Roberts, W. K.;C. P. Selitrennikoff
  17. J. Microbiol. Biotechnol. v.10 New polyene macrolide antibiotics from Streptomyces sp. M90025 Seo. Y. W.;K. W. Cho;H. S. Lee;T. M. Yoon;J. H. Shin
  18. Biochim. Biophys. Acta v.1430 A new antifungal peptide from the seeds of Phytolacca americana: Characterization, amino acid sequence and cDNA cloning Shao, F.;Z. Hu;Y. M. Xiong;Q. Z. Huang;C. G. Wang;R. H. Zhu;D. C. Wang
  19. J. Microbiol. Biotechnol. v.9 Structure and antibiotic activity of fragment peptide of antifungal protein isolated from Aspergillus gigateus Shin, S. Y.;J. H. Kang;D. G. Lee;Z. Z. Jin;S. Y. Jang;K. L. Kim;K. S. Hahn
  20. J. Biol. Chem. v.267 Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds Terras, F. R. G.;H. M. E. Schoofs;M. F. C. De Bolle;F. Van Leuven;S. B. Rees;J. Vanderleyden;B. P. A. Cammue;W. F. Broekaert
  21. FEBS Lett. v.316 A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species Terras, F. R. G.;S. Torrekens;F. Van Leuven;R. W. Osborn;J. Vanderleyden;B. P. A. Cammue;W. F. Broekaert
  22. Mol. Plant-Microbe Interact. v.4 A new family of plant antifungal proteins Vigers, A. J.;W. K. Roberts;C. P. Selitrennikoff
  23. Biochem. Biophys. Res. Commun. v.269 Dolichin, a new chitinase-like antifungla protein isolated from fried beans (Dolichos lablab) Ye, X. Y.;H. X. Wang;T. B. Ng