Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Glycyrrhetinic Acid Inhibits Cell Growth and Proliferation, and Induces Apoptosis in Human Gingival Fibroblasts

Kim, Kang-Ju;Yoo, Soo-Kyoung;Kim, In-Sook;Kim, Shin-Moo;Lee, Su-Jong;Yu, Hyeon-Hee;You, Yong-Ouk

  • Published : 20021000
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Abstract

Effects of GA on the cell growth and proliferation, collagen synthesis, and apoptosis of cultured human gingival fibroblasts were examined. Cell growth was inhibited at higher than 50 ${\mu}g/mL$ GA, and total collagen in the cell decreased at 50-100 ${\mu}g/mL$ of GA compared to the control group in a dose-dependent manner. When human gingival fibroblasts were cultured with 100 ${\mu}g/mL$ of GA for 1 day, diploid cells with a mean fluorescence over channel 80 decreased and cells with reduced acridine orange binding (fluorescence below channel 80) increased. To study whether GA-treated human gingival fibroblast death results from apoptosis, DNA fragmentation in human gingival fibroblasts was determined at I day after 50 or 100 ${\mu}g/mL$ of GA treatment. When DNA extracted from the control group and 50 ${\mu}g/mL$ of GA-treated group were analyzed by gel electrophoresis, fragmentation was not observed. In contrast, DNA from 100 ${\mu}g/mL$ of GA-treated cells demonstrated a characteristic "ladder" pattern of DNA fragment. Data indicate that 100 but not 50 ${\mu}g/mL$ of GA might have an effect on the apoptosis of human gingival fibroblasts. These results suggest that GA may inhibit the cell growth and proliferation of human gingival fibroblasts including collagen synthesis, and might be useful for the control of gingival hyperplasia at 50 ${\mu}g/mL$.

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References

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