Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Medium for Griseofulvin Production: Part II. Optimization of Medium Constituents Using Central Composite Design

  • Dasu, Venkata (Biotechnology Research Centre, Department of Chemical Engineering, Indian Institute of Technology-Madras) ;
  • Panda, V.T. (Biotechnology Research Centre, Department of Chemical Engineering, Indian Institute of Technology-Madras) ;
  • Chidambaram, M. (Biotechnology Research Centre, Department of Chemical Engineering, Indian Institute of Technology-Madras)
  • Published : 2002.06.01
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Abstract

Central composite experimental design was employed to determine the optimal concentration of medium constituents for griseofulvin production by Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvum MTCC 2004. The optimal concentration of sucrose, $K_2HPO_4,\;NaNO_3,\;and\;FeSO_4{\cdot}7H_2O$ were found to be 48.08 g/1, 1.228 g/1 , 2.7 g/1, and 0.011 g/1, respectively, for Penicillium griseofulvum MTCC 1898, and for Penicillium griseofulvum MTCC 2004, 23.52 g/1, 43.67 g/1, and 0.0434 g/1 of glucose, lactose, and $MnSO_4{\cdot}H_2O$, respectively. The yield of griseofulvin under optimal composition of medium constituents increased by 1.26 and 1.38 times than prior to optimization, for Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvutn MTCC 2004, respectively.

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References

  1. Bioproc. Eng. v.20 Propoinic acid productio by whole cells of Propoinibacterium freudenreichii Balamuragam, K.;V. Venkata Dasu;T. Panda
  2. J. Roy. Statist. Soc. v.B13 On the experimental attainment of optimum conditions Box, G.E.P.;K.B. Wilson
  3. Proc. Biochem. v.29 Response-surface methodology for optimization of citric acid formentation by Aspergillus foetidus Chen, H. C.
  4. J. Phar. Pharmcol. v.15 A rapod method for the determination of griseofulvin in fermentation broth Holbrook, A.;F. Bailey;G.M. Bailey https://doi.org/10.1111/j.2042-7158.1963.tb11222.x
  5. Bioproc. Eng. v.15 Optimization of carbon and nitrogen sources in the medium and environmental faceors for enhanced production of chitinase by Trichoderma harzianum Kapat, A.;S. D. Takshit;T. Panda. https://doi.org/10.1007/BF00435522
  6. Bioproc. Eng. v.19 Optimization of medium by Gluconobacter suboxydans Manta, D.;Z.A. Basha;T. Panda
  7. Statistical Design and Analysis of Experiments - With Applications to Engineering and Science Mason, R.L.;R.F. Gunst;J.L. Hess
  8. Computer J. v.3 An automatic method for finding the freatest of least value of a function Rosenbrock, H.H. https://doi.org/10.1093/comjnl/3.3.175
  9. Ind. Eng. Chem. v.58 Efficient screening of process variables Stowe, R. A.;R. P. Mayer.
  10. Enzyme Microbiol. Technol. v.21 Response surface analysis of chlortetracycline and tetracycline production with K-carrageenan immobilized Wtreptomyces aureofaciens Teruel, M.L.A.;E. Gontier;C. Bienaime;J.E.N. Saucedo;J. N. Barbotin https://doi.org/10.1016/S0141-0229(97)00045-8
  11. J. Microbiol. Biotechnol. v.12 Development of medium for griseofulvin production: Screening of medium constituents using Plackett-Burman experimental desing Wenkata Dasu, V.;T. Panda;M. Chidambarm
  12. Bioproc. Eng. v.22 Optimization of microbiological paramenters for enhanced griseofulvin production using response surface methodology Wenkata Dasu, V.;T. Panda https://doi.org/10.1007/PL00009099