Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Determination of Optimum Aggregates of Porcine Hepatocytes As a Cell Source of a Bioartificial Liver

  • Lee, Doo-Hoon (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Lee, Ji-Hyun (Division of Food and Biotechnology, Pukyong National University) ;
  • Choi, Jeong-Eun (Lifecord Biomedical Research Center, Lifecord Inc.) ;
  • Kim, Young-Jin (Lifecord Biomedical Research Center, Lifecord Inc.) ;
  • Kim, Sung-Koo (Division of Food and Biotechnology, Pukyong National University) ;
  • Park, Jung-Keug (Department of Chemical and Biochemical Engineering, Dongguk University)
  • Published : 2002.10.01
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Abstract

Large quantities of porcine hepatocyte aggregates with various degrees of aggregation (DA) could be obtained by controlling the suspension periods (0,9,24, and 48 h), and by entrapping the hepatocyte aggregates in model materials of encapsulation such as Ca-alginate and type-I collagen gels. The effects of DA on liver-specific functions of hepatocytes were evaluated in order to obtain optimum DA for the cell source of bioartificial liver (BAL) systems. Irregular rugged aggregates (size $75 \pm 28$ $\mu\textrm{m}$) farmed by 24 h of suspension culturing showed peak viability and hepatic functions such as ammonia removal and albumin secretion in the two types of entrapment systems, thus offering themselves as a stable cell source of a BAL system for hepatic functions and scale-up.

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