KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production and Chracteristics oil Antifungal agents from Bacteria

세균으로부터 항진균성 물질의 생산 및 특성

  • 김현수 (계명대학교 미생물학과) ;
  • 육영민 ((주)계명 바이오테크) ;
  • 여수환 (계명대학교 전통미생물자원개발 및 산업화연구센터)
  • Published : 2003.12.01
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Abstract

For the production of antifungal compound, strain B-1 was used as a strong producing strain among bacteria isolated from various soil samples. The optimum medium for the production of antifungal compound was PDB (potato starch 0.4%, dextrose 2％, pH5.1). The optimum conditions for the production of antifungal compound didn't affect on the carbon and nitrogen sources. The produced compound showed broad antimicrobial activity to the tested strains such as five fungi and four bacteria. The optimum pH and temperature of the production antifungal compound were pH 5.0 and 28$^{\circ}C$, respectively. Ether extrct (1$\mu\textrm{g}$/${\mu}\ell$) of culture broth was confirmed inhibitory zone by the thin layer chromatography and plate assay. The antimicrobial compound was unstabled after heat (121$^{\circ}C$) trsatment. Strain B-1 was mass cultured in a 5-liter tormentor, containing 3 liters of PDB medium at 28$^{\circ}C$, pH 5.0, 120 (pm with aeration (1L/min).

토양으로부터 분리한 균주 중 항진균 활성이 우수한 균주를 선발하여 B-1이라 명명하였다. B-1의 항균성 물질 생산에 대한 배양조건을 검토하기 위해 최적배지, 탄소원, 질소원, pH, 온도별로의 영향을 조사하였다. PDB 배지에서 항균력이 우수하였으며 탄소원$.$질소원에는 별다른 영향이 없었다. pH와 온도에서는 각각 pH 5.0, 28$^{\circ}C$에서 항균력이 우수하였다. 항균 활성은 진균에서 강하게 나타났으며 일부 세균에서도 항균력을 나타내었다. 항진균성 물질은 산, 알칼리 처리 후 잔존 활성은 70∼80%로 나타났으며 열처리시 완전히 실활되어 열에 대해 상당히 불안정하다는 것을 확인하였다. 항균성 물질위 용매 전용성을 위해 TLC를 통하여 분석하였으며 plate assy를 실시하여 항균성물질을 확인하였다. 항진균성 물질의 대량생산을 위해 기본배지인 PDB를 직접 실험실 내에서 제조하였으며 시판의 PDB와 비교하였을때 항균성 물질 생산면에서나 가격면에서 더 나은 결과를 보였다.

Keywords

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