KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Introgression of Oryza minuta into Rice, Oryza sativa

벼 Oryza sativa x O. minuta 여교배 계통에서 이입 염색체단편 검정

  • Jin Feng Xue (Department of Agronomy, Chungnam National University) ;
  • Kang Kyung-Ho (Genetics & Breeding Division, National Institute of Crop Science) ;
  • Kwon Soo-Jin (National Institute of Agricultural Biotechnology) ;
  • Jeong Oh-Young (Genetics & Breeding Division, National Institute of Crop Science) ;
  • Le Heung Linh (Department of Agronomy, Chungnam National University) ;
  • Moon Huhn-Pal (Genetics & Breeding Division, National Institute of Crop Science) ;
  • Ahn Sang-Nag (Department of Agronomy, Chungnam National University)
  • 김봉학 (충남대학교 농업생명과학대학 농학과) ;
  • 강경호 (작물과학원 유전육종과) ;
  • 권수진 (농업생명공학연구원) ;
  • 정오영 (작물과학원 유전육종과) ;
  • 리흥린 (충남대학교 농업생명과학대학 농학과) ;
  • 문헌팔 (작물과학원 유전육종과) ;
  • 안상낙 (충남대학교 농업생명과학대학 농학과)
  • Published : 2004.12.01
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Abstract

An introgression line, WH79006 was produced from a single plant from $BC_5F_3$ families from a cross between Hwaseongbyeo used as a recurrent parent and O. minuta (BBCC, Ace. No. 101154) as a donor parent, which was subsequently self-pollinated for three generations. WH79006 resembled the O. sativa parent, Hwaseongbyeo. However it differed from Hwaseongbyeo in several traits including days to heading, culm length, grain size, spikelets per panicle and fertility. These differences in the traits between WH79006 and Hwaseongbyeo can be attributed to the O. minuta introgressions. To detect the introgressions, 294 SSR markers of known chromosomal position have been used. At least, 28 introgressed chromosomal segments have been identified using SSR markers and they map to all chromosomes except chromosome 2. The size of the introgressed segments ranged from 4 to 35cM. A QTL related to culm length was detected using 75 $F_2$ plants from the Hwaseongbyeo/WH79006 cross. This QTL, cl6 located on chromosome 6 explained $9.6\%$ of the total phenotypic variation in the population. This QTL has not been detected in the previous QTL studies between Oryza sativa cultivars, indicating potentially novel alleles from O. minutan.

화성벼와 O. minuta 간 여교잡을 통해 반복친인 화성벼와 출수기, 간장 등 여러 작물학적 특성에서 뚜렷한 차이를 보이는 염색체단편 치환계통 "WH79006"을 육성하였는데 화성벼와 WH79006의 차이는 WH79006에 이입된 O. minuta 염색체 단편에 의한 것이라고 할 수 있다. WH79006이 화성벼의 유전적 배경에 O. minuta의 어느 염색체 단편을 가지고 있는지를 검정하기 위해 SSR마커를 이용하였다. 벼 염색체에 골고루 분포한 294개의 SSR 마커를 검정한 결과 최소한 28개의 이입 단편을 검정하였는데 이들은 2번 염색체를 제외한 모든 염색체에 분포하였으며 전체 길이는 약 168cm이었다. 간장 관여 QTL을 탐색하기 위해 화성벼/WH79006 조합의 75개 $F_2$ 개체를 육성, 간장을 조사하였다. QTL분석 결과 6번 염색체의 RM225부근에서 간장에 관여하는 QTL cl6가 탐지되었다. cl6는 전체 표현형변이의 $9.6\%$를 설명하였으며 O. minute의 대립유전자가 간장을 크게 하는 방향으로 작용하였다. 이 결과는 O. minuta가 포복형임에도 불구하고 간장 조절 유전자들 중에는 간장을 크게 하는 방향으로 작용하는 유전자가 있음을 제시하고 있으며, 이 유전자들은 앞으로 QTL 분석을 통해 그 작용을 밝힐 예정이다. 추후교잡 집단을 이용 O. minuta 특이적 단편들이 어느 형질과 관련이 있는지를 밝힐 예정이다.

Keywords

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