Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Karyotype Analysis and Physical Mapping of rDNAs Using McFISH in Jeffersonia dubia Benth

깽깽이풀의 핵형분석과 McFISH를 이용한 rDNA의 물리지도 작성

  • Kim, Soo-Young (School of Bioscience & Biotechnology, Chungnam Natl. Univ.) ;
  • Choi, Hae-Woon (School of Bioscience & Biotechnology, Chungnam Natl. Univ.) ;
  • Koo, Dal-Hoe (School of Bioscience & Biotechnology, Chungnam Natl. Univ.) ;
  • Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute) ;
  • Bang, Jae-Wook (School of Bioscience & Biotechnology, Chungnam Natl. Univ.)
  • 김수영 (충남대학교 생명과학부) ;
  • 최혜운 (충남대학교 생명과학부) ;
  • 구달회 (충남대학교 생명과학부) ;
  • 김찬수 (산림과학원 난대산림연구소) ;
  • 방재욱 (충남대학교 생명과학부)
  • Published : 2005.02.28
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Abstract

Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged $1.95{\sim}3.50\;{\mu}m$. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.

보호식물이며, 약용식물인 깽깽이풀 (Jeffersonia dubia)을 대상으로 핵형 분석과 McFISH 기법을 이용한 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 체세포 염색체 수는 2n=2x=12였으며, 2쌍의 중부 염색체 (염색체 1, 3), 2쌍의 차중부 염색체 (염색체 2, 4) 그리고 2쌍의 차단부 염색체 (염색체 5, 6)로 구분되었고, 염색체의 평균 길이는 $1.95{\sim}3.50{\mu}M$이었다. McFISH기법을 이용하여 45S와 5S rDNA의 염색체상의 위치를 확인한 바, 3쌍의 45S rDNA signal은 4번, 5번 그리고 6번 염색체의 단완 말단 부위에서 관찰되었고, 한 쌍의 5S rDNA signal은 2번 염색체의 동원체 부위에서 관찰되었다.

Keywords

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