Germination Effect of Soybean on Its Contents of Isoflavones and Oligosaccharides

  • Kim, Woo-Jung (Department of Food Science and Technology, Sejong University) ;
  • Lee, Hye-Yeon (Department of Food Science and Technology, Sejong University) ;
  • Won, Moo-Ho (Department of Anatomy, Hallym University) ;
  • Yoo, Sang-Ho (Department of Food Science and Technology, Sejong University)
  • Published : 2005.08.30

Abstract

Three Korean soybean varieties - Shinpaldal-2, Seomoktae and Seoritae - were investigated for changes in their physical properties and the amount of functional components (i.e. isoflavones and oligosaccharides), during germination. Soybeans were germinated at $20^{\circ}C$ for 96 hr in complete darkness. The dry weights of cotyledone, hypocotyl, seed coat, and hilum of Seoritae were heavier than those of other varieties. The dry weights of the three bean varieties decreased steadily in spite of root growth. The largest amount of isoflavone content was observed from Shinpaldal-2 (1.824 mg/g), followed by Seoritae (1.216 mg/g) and Seomoktae (1.125 mg/g). Total isoflavone content increased by 13% during initial germination, and then decreased thereafter. Aglycone types such as daidzein and genistein dominated the increase in isoflavone contents. The increase in genistein content of Shinpaldal-2 was 17.5 fold compared with ungerminated soybean, while the amount of daidzein was 6.7 times as much as ungerminated Shinpaldal-2 over an 18-hr germination period. Oligosaccharide contents such as raffinose (Raf) and stachyose (Sta) rapidly decreased during germination, while the sucrose (Sue) content remained constant until 36-48 hr of germination. From these results, it was clearly shown that the germination process significantly changed the contents of functional nutrients in soybeans. Therefore, the optimization of germination process should be considered to improve the biological functionality of soybeans in food processing.

Keywords

References

  1. Nutr. Cancer v.21 Soy intake and cancer risk; A review of the in vitro and in vivo data Messina, M.J.;Persky, V.;Setchell, K.D.R.;Barnes, S.
  2. Proc. Soc. Exp. Biol. Med. v.217 Evolution of the health benefits of soy isoflavones Barnes, S.
  3. J. Clin. Endocr. Metab. v.83 Potential health benefits of dietary phytoestrogens: A review of the clinical, eqidemiological, and mechanistic evidence Tham, D.M.;Gardner, C.D.;Haskell, W.L.
  4. Br. J. Nutr. v.79 Phytoestrogens: Where are we now? Bingham, S.A.;Atkinson, C.;Liggins, J.;Bluck, L.;Coward, A.
  5. Lancet v.337 Dietary effects on breast-cancers in Singapore Lee, H.P.;Gourley, L.;Duffey, S.W.;Esteve, J.;Lee, J.;Day, N.E.
  6. Lancet v.3444 The oxidation hypotheses of atherosclerosis Witztum, J.L.
  7. J. Nutr. Biochem. v.13 Soy isoflavones' osteoprotective role in postmenopausal women: mechanism of action Arjmandi, B.H.;Smith, B.J.
  8. Lancet v.339 Dietary phyto-estrogens and the menopause in Japan Aldercreutz, H.;Hamalainen, E.;Gorbachh, S.;Goldin, B.
  9. Bifidobacteria Microflora v.5 Effect of fructooligosaccharides on intestinal flora and human health Hidaka, H.;Eida, T.;Takizawa, T.;Tokunaga, T.;Tashiro, Y.
  10. Bifidobacteria Microflora v.9 Effect of xylooligosaccharides on the growth of bifidobactera Okazaki, M.;Fujikawa, S.;Mtsumoto, N.
  11. Korea J. Food Technol. v.27 Physical and physiological properties of isomaltooligosaccharides and fructooligosaccharides Kim, J.R.;Yook, C.;Kwon, H.K.;Hong, S.Y.;Park, C.K.;Park, K.H.
  12. J. Jpn. Soc. Nutr. Food Sci. v.44 Change of soybean oligosaccharides in the digestive tract Kato, Y.;Ikeda, N.;Iwanami, T.;Ozaki, A.;Ohmura, K.
  13. RDA J. Agri. Sci. v.36 Characteristics of soy sprouts cultivated with soybeans for sprout Kim, Y.H.;Kim, S.D.;Hong, E.H.
  14. J. Korean Agric. Chem. Soc. v.23 Changes in nitrogen compounds in soybean sprout Yang, C.B.;Kim, Z.U.
  15. J. Korean Agric. Chem. Soc. v.25 Studies on the effects of plant growth regulator on growth and nutrient compositions in soybean sprout Lee, S.H.;Chang, D.H.
  16. Lebensm. Wiss. U. Technol. v.6 The removal of oligosaccharides from soybeans Kim, W.J.;Smit, C.J.B.;Nakayama, T.O.M.
  17. J. Food Sci. v.48 Effects of germination on proteins, raffinose oligosacchrides and antinutritional factors in the Great Northern Beans Sathe, S.K.;Dehpande, S.S.;Reddy, N.R.;Goll, D.E.;Salunkhe, D.K.
  18. Food Sci. Biotechnol. v.12 Changes in composition of pea during germination, microwave treatment and drying Kadlec, P.;Skullinova, M.;Kaasova, J.;Bubnik, Z.;Pour, V.;Dostalova, J.;Valentova, H.;Hosnedl, V.
  19. Food Sci. Biotechnol. v.13 Extraction of isoflavones from soybean hypocotyls using aqueous ethanol Choi, Y.B.;Rhee, J.S.;Lee, Y.B.;Nam, S.Y.;Kim, K.S.
  20. Korean J. Food Sci. Technol. v.27 Ultrafiltration of soybean cooking water for the production of soy-oligo-saccharides Mok, C.K.;Ku, K.H.;Park, D.J.;Kim, N.;Sohn, H.S.
  21. J. Food Sci. v.57 Isoflavone aglucones and volatile organic compound in soybeans; effect of soaking treatments Ha, E.Y.W.;Morr, C.V.;Seo, A.
  22. J. Chromatogr. B. v.777 Bioavailability of isoflavones Hendrich, S.
  23. Phytochemistry v.58 Partial purification and characterization of a soybean ${\beta}-glucosidase$ with high specific activity towards isoflavone conjugates Hsieh, M.C.;Graham, T.L.
  24. Korean J. Breed. v.34 Varietal variation of oligosaccharides during germination in soybean Kim, S.L.;Moon, J.K.;Yun, H.T.;Park, K.Y.;Lee, Y.H.;Ryu, Y.H.;Ku, J.H.;Kim, S.D.
  25. J. Food Sci. v.46 Microbial ${\alpha}-galactosidase$ for soymilk processing Cruz, R.;Batistela, J.C.;Wosiacki, G.