Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimal Criterion for the Scale-Up Production of Schizophyllan in the Stirred Tank Reactor

  • KWAK, JUNG-KI (Institute for Biotechnology, Technical University of Berlin) ;
  • KOO, JAE-GUEN (College of Ocean Science and Technology, Kunsan University) ;
  • PARK, SUNG-WOO (College of Ocean Science and Technology, Kunsan University) ;
  • CHO, MAN-GI (Engineering Research Center, Dongseo University) ;
  • KANG, BYEONG-CHUL (Engineering Research Center, Dongseo University) ;
  • RAINER BUCHHOLZ (Institute for Biotechnology, Technical University of Berlin) ;
  • PETER GOETZ (Institute for Biotechnology, Technical University of Berlin)
  • Published : 2005.02.01
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Abstract

Optimal criterion for the scale-up production of schizophyllan, a fungal polysaccharide secreted by Schizophyllum commune, was investigated. For the production of the polysaccharide in a 150-l bioreactor, the culture conditions optimized in a 15-l bioreactor were applied to a 150-l bioreactor with scale-up process, by changing impeller speed and airflow rate. The optimized impeller speed in the 15-l bioreactor was 50 rpm in a technical medium based on barley. For establishment of the scale-up process, 3 kinds of criteria were used while the gas throughput number was kept constant, as follows; constant volume-related power input, constant tip speed of stirrer, and constant Reynolds number. In the 150-l bioreactor, the highest values for the maximum specific growth rate (1.17/day) and productivity (0.63 g/L${\cdot}$day) were achieved in the culture condition from constant volumerelated power input criterion.

Keywords

References

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