Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

In vitro Mass Propagation of Ardisia pusilla DC.

산호수 (Ardisia pusilla DC.)의 기내 대량번식

  • Kang Gwan-Ho (Ohyoonbiotech Co. College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Oh Owel-Sun (Ohyoonbiotech Co. College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Goo Dae-Hoe (National Horticultural Research Institute, RDA) ;
  • Eun Jong-Seon (College of Agriculture & Life Sciences, Chonbuk National University) ;
  • Kim Hyung-Moo (College of Agriculture & Life Sciences, Chonbuk National University)
  • 강관호 (오윤바이오텍, 전북대학교 농업생명과학대학) ;
  • 오월선 (오윤바이오텍, 전북대학교 농업생명과학대학) ;
  • 구대회 (원예연구소 화훼과) ;
  • 은종선 (전북대학교 농업생명과학대학) ;
  • 김형무 (전북대학교 농업생명과학대학)
  • Published : 2005.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

To establish the mass proliferation system of Ardisia pusilla DC, the shoot tips of Ardisia pusilla DC were cultured on the MS and half-strength MS medium supplemented with $0{\sim}5.0$ mg/L BA or $0{\sim}0.5$ mg/L thidiazuron(TDZ), respectively. A few multiple shoot formation observed when the shoots were cultured on MS medium containing TDZ. However, the frequency of multiple shoot formation was reached up to 82.4%, when the shoots were cultured on half-strength MS medium supplemented with 0.5 mg/L BA. Also the number of shoot per explant was 7.1. To promote rooting from multiple shoot, newly formed shoots were transferred to half-strength MS medium containing 0.5 mg/L IBA or 0.5 mg/L NAA, respectively. Regenerated plantlets were grown to normal mature plants in soil.

우리나라 자생식물인 산호수 (Ardisia pusilla DC)를 기내에서 대량증식하기 위하여 초대배양으로 산호수 경정을 MS, 1/2MS 배지에 BA와 TDZ의 농도를 달리하여 첨가된 배지에 치상하여 배양하였다. 무기염류를 달리한 배지와 생장조절물질 농도를 다르게 한 배지 중 1/2MS 배지에 BA 0.5mL가 첨가된 배지가 신초 수 7개 이상으로 증식이 양호하여 산호수의 초대배양에 적정배지로 나타났다. TDZ를 첨가한 배지에서는 캘러스형성이 과다하여 부정아 형성을 오히려 방해하여 BA 첨가배지보다 신초증식이 양호하지 못하였다. 신초의 증식을 위해 1/2 MS 배지에 BA 0.5 mg와 sucrose의 농도를 달리하여 첨가한 결과, sucrose 농도 20 g/L를 첨가한 배지에서 신초의 증식, 생장 및 다아체 형성이 양호하였다. 형성된 신초의 발근은 1/2MS 배지에 IBA, NAA를 각각 첨가한 배지를 사용하였는데 두 가지 옥신류에서 0.5 mg의 농도에서 신초의 생장과 발근이 양호하였다. 잎과 뿌리가 잘 발달한 유식물체를 원예용 상토에 옮겨 키웠을 때 모든 개체가 정상적인 형태로 성장하였다.

Keywords

References

  1. Bae KH, Kim YS, Jeong JH, Kim YS, Choi YE, Yoon ES (2004) Induction of hariy root and bioreactor culture of Lysium chinense. Kor J Plant Biotech. 31: 295-300 https://doi.org/10.5010/JPB.2004.31.4.295
  2. Earle ED, Langhans RW (1974) Propagation of Chrysanthemum in vitro. I. Multiple plantlets from shoot tips and the establishment of tissue culture. J Amer Soc Hort 99: 128-132
  3. Gribaudo I, Fronda A (1991) Effects of thidiazuron on grapevine axillary buds cultivated in vitro. Hort Sci 26: 103
  4. Hackett WP (1985) Juvenility, maturation and rejuvenation in wood plants. Hort Rev 7: 109-158
  5. Han BH, Joung HY, Ko JY (1997) In vitro propagation of Ficus benjamina by shoot tip culture. J Kor Soc Hort Sci 38: 315-319
  6. Han BH, Yae BW, Goo DH, Yu HJ (2004) Micropropagation of Philodendron wend-imbe through adventitious multi-bud cluster formation. Kor J Plant Biotechnol 31: 115-119 https://doi.org/10.5010/JPB.2004.31.2.115
  7. Kim TJ (1996) Korean Resources PlantsIII. Seoul National University Publisher, Korea
  8. Kwon JH, Chen HC, Yang DC (2003) Production of ginsenoside in callus of ginseng hairy roots J Ginseng Res 27: 78-85 https://doi.org/10.5142/JGR.2003.27.2.078
  9. Kusey WE, Hammer PA, Weiler TC (1980) In vitro Propagation of Gypsophila paniculata L ‘Bristol Fairy’. Hort-Science 15: 600-601
  10. Lee CH, Kwon OK, Kim YJ (2005) Rooting characteristics of stem tip cuttings in Ardisia pusilla as influenced by cutting stage, rooting medium, temperature, and plant growth regulator pretreatment. J Kor Soc Hort Sci 46 : 217-224
  11. Lee SA (2002) A study on the characteristics and propagation method of Ardisia pusilla DC. Master’s Thesis, Sungkyunkwan university.
  12. Liu S, Zhong JJ (1997) Simultaneous production of ginseng saponin and polysaccharide by suspension cultures of Panax ginseng: Nitrogen effect. Enzyme Microb Technol 21: 518-524 https://doi.org/10.1016/S0141-0229(97)00023-9
  13. Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol 29: 92-96 https://doi.org/10.1007/BF02632259
  14. Mok MC, Mok DWS, Tumer JE, Mujer CV (1987) Biological and biochemical effects of cytokinin active phenylurea derivatives in tissue culture systems. Hort Sci 22: 1194-1196
  15. Murashige T, Skoon F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Pennazio S (1975) Effect of adenine and kinetin of development of carnation tips cultured in vitro. J Hort Sci 50: 161-164
  17. Reynolds JF (1987) Chemical regulation in tissue culture; An overview. Hort Sci 22: 1192-1194
  18. Takayama S, Misawa M (1892) Regulation of organ formation by cytokinin and auxin in Lilium bulb scales grown in vitro. Plant Cell Physiol 23: 67-74
  19. Tegeder M, Gebhardt D, Schieder O, Pickardt T (1995) Thidiazuron induced plant regeneration from protoplasts of Vicia faba cv. Mythos. Plant Cell Rep 15: 164-169 https://doi.org/10.1007/BF00193712