Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Growth-inhibitory Effects of the Plocamium telfairiae Extracts on Cancer Cells

참곱슬이(Plocamium telfairiae) 추출물의 암세포 성장억제 효과

  • Kim, Ju-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Ji-Hwan (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Cha, Mi-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Choi, Byeong-Dae (Division of Marine Bioscience, Gyeongsang National University) ;
  • Choi, Sun-Uk (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Yong-Il (Department of Food Science and Biotechnology, Kyungnam University)
  • 김주영 (경남대학교 식품생명학과) ;
  • 황지환 (경남대학교 식품생명학과) ;
  • 차미란 (경남대학교 식품생명학과) ;
  • 최병대 (경상대학교 해양생물이용학부) ;
  • 최선욱 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과) ;
  • 황용일 (경남대학교 식품생명학과)
  • Published : 2006.07.31
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Abstract

The extracts of Plocamium telfairiae using several solvents with different polarities were prepared and their growth inhibitory effects were examined on the human cancer cells. We investigated the cytotoxic effects of P. telfairiae extracts on HT-29 cells by the MTT reduction assay and examining the morphological change under the inverted microscope. Among three extracts, the methanol extract showed the strongest inhibitory effect on the growth of HT-29 cells. The methanol extract was further fractionated sequentially with n-hexane, diethyl ether, ethyl acetate, and aqueous for purifying crude methanol extract. The n-hexane layer among the fractioned layers showed remarkable inhibitory activity on the growth of HT-29 cells. Moreover n-hexane layer showed the notable growth inhibition effects with a dose-dependent manner against SW620, HeLa, and MCF-7 cells as well as HT-29 cells. These results indicated that P. telfairiae extracts may be contained bioactive materials with inhibitory effect on the growth of human cancer cells.

해조류로부터 항암활성을 가지는 생리활성물질을 탐색하기 위하여 홍조해조류의 하나인 참곱슬이를 용매별 추출한 후, 분획하여 암세포 성장 억제효과를 연구하였다. 참곱슬이를 이용하여 대장암 세포주 HT-29에 대해 methanol, ethanol 및 acetone 추출물을 처리했을 때 모두 농도에 의존적으로 성장저해 효과가 나타났고, 그 중에서도 methanol 추출물에서는 50 mg/ml 농도 처리 시 12% 이하의 생존률을 보이는 가장 강한 암세포 성장 억제효과를 보였다. 또한 HT-29 암세포 성장에 대한 현미경 관찰 결과 methanol 추출물은 대조구에 비하여 암세포 성장 억제 정도와 세포의 수축등 형태변화가 두드러지게 나타났다. 그리고 가장 활성이 좋은 methanol 추출물을 용매 분획하여 얻은 분획물 중 n-hexane과 diethyl ether 분획물에서 암세포 성장 억제효과를 보였으며, n-hexane 분획물이 HT-29 세포주에 대하여 가장 높은 항암활성을 나타내었다. 따라서 n-hexane 분획물에 대한 여러 인체 암세포주에 대해 항암활성을 확인하기 위하여 HT-29와 같은 대장암 유래의 세포주 SW620, 자궁경부암 세포 HeLa 및 유방암세포 MCF-7에 대한 암세포 성장 억제효과를 확인한 결과, 3종의 암세포주에서 모두 농도 의존적으로 높은 항암활성을 보임에 따라 참곱슬이에 여러 인체 암세포 성장 억제효과를 가지는 생리활성 물질이 함유되어 있음을 알 수 있다.

Keywords

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