Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Cytotoxic Activity of the Inula japonica Extracts Against Several Human Cancer Cell Lines In Vitro

선복화 (Inula japonica)추출물의 암세포주에 대한 In Vitro 세포독성

  • Cha, Mi-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Ju-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Ji-Hwan (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 차미란 (경남대학교 식품생명학과) ;
  • 김주영 (경남대학교 식품생명학과) ;
  • 황지환 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Published : 2006.09.30
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Abstract

The present study describes the preliminary evaluation of the cytotoxic activity of the extracts from Inula japonica. I. japonica was extracted with methanol, ethanol, acetone, and water, and then cytotoxic activity of these extracts were evaluated. The cytotoxic activity of each extract was assessed by the MTT-dye reduction assay. Both ethanol and acetone extracts from I. japonica showed the cytotoxic activity against the HT-29 human colon cancer cells. Furthermore, the ethanol extract was fractionated with n-hexane, diethyl ether, ethyl acetate, and water according to degree of Polarity, The diethyl ether fraction showed the highest cytotoxic activity against HT-29 cells, but the other fractions showed low cytotokic activity. In addition, diethyl ether layer also showed the cytotoxic activity against various tumor cells, such as human colon carcinoma SW620, human cervix adenocarcinoma HeLa, and human breast adenocarcinoma MCF-7 cells as well as HT-29 cells. These studies support that extracts of I. japonica may be a potential candidate as possible chemotherapeutic agent against human cancer.

Keywords

References

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