Physicochemical Characteristics of Garlic (Allium sativum L.) on the High Temperature and Pressure Treatment

고온고압처리에 따른 마늘(Allium sativum L.)의 이화학적 특성

  • Kwon, Oh-Chan (Department of Food Science and Technology, Chungbuk National University) ;
  • Woo, Koan-Sik (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Tae-Myoung (College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Dae-Joong (College of Veterinary Medicine, Chungbuk National University) ;
  • Hong, Jin-Tae (College of Pharmacy, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Published : 2006.06.01

Abstract

Effects of high temperature and pressure treatment on garlic (Allium sativum L.) treated at 110, 120, 130, 140, and $150^{\circ}C$ for 1, 2, 3, 4, and 5 hr were evaluated by investigating changes in antioxidant activities, and total polyphenol, total flavonoid, and 5-hydroxymethyl-2-furaldehyde (5-HMF) contents of garlic juice. Antioxidant activities, and total polyphenol, total flavonoid, and 5-HMF contents increased with increasing treated temperature and time. $IC_{50}$ (1.952 mg/g) was achieved at $150^{\circ}C$ for 4 hr, 50 times higher than untreated garlics. Highest total polyphenol content was 18.155 mg/g at $140^{\circ}C$ and 2 hr, seven times higher than untreated garlics. Highest total flavonoid content was $532.73\;{\mu}g/g$ at $150^{\circ}C$ and 1 hr, sixteen times higher than untreated garlics. 5-HMF content was highest (7,765.9 ppm) at $150^{\circ}C$ and 2 hr. Correlation coefficients among antioxidant activities, and total polyphenol, total flavonoid, and 5-HMF contents were very high (p<0.01).

고온고압처리 조건에 따른 마늘의 이화학적 특성의 변화와 유용성분 변화를 확인하기 위하여 고온고압처리온도(110, 120, 130, 140 및 $150^{\circ}C$)와 시간(1, 2, 3, 4 및 5 시간)을 변수로 처리한 후 DPPH assay에 항산화활성$(IC_{50})$, 총 폴리페놀 함량, 총 플라보노이드 함량 및 5-HMF를 측정하였고 이틀의 상관관계를 분석하였다. 마늘은 항산화활성$(IC_{50})$은 고온고압처리 온도와 시간의 증가에 따라 증가하였으며, $150^{\circ}C$, 4시간 처리구에서 1.95 mg/g으로 가장 낮아 생마늘에 비해 약 50배 이상 증가하였다. 총 폴리페놀 함량은 고온고압처리 온도와 시간의 증가에 따라 증가하였으며, $140^{\circ}C$, 2시간에서 $18.16{\pm}0.51\;mg/g$으로 생마늘에 비해 약 7배정도 증가하였다, 총 플라보노이드 함량도 고온고압처리 온도와 시간의 증가에 따라 증가하였으며, $150^{\circ}C$, 1시간에서 $532.73{\pm}18.79\;{\mu}g/g$으로 생마늘보다 약 16배 높은 함량을 나타내었다. 5-HMF 함량은 110 및 $120^{\circ}C$에서는 변화가 작았지만 $130^{\circ}C$에서는 고온고압처리 시간의 증가에 따라 급격한 증가를 보였고 $150^{\circ}C$, 2시간에서 $7,765.9{\pm}359.6\;ppm$로 가장 높은 함량을 나타내었다. 통계분석 결과 고온고압처리온도와 시간이 항산화성, 폴리페놀, 플라보노이드 및 5-HMF 함량에 유의적으로 영향을 미치며(p<0.001), 처리 시간보다 온도가 더 많은 영향을 미치는 것으로 나타났다. 또한 항산화성, 폴리페놀, 플라보노이드 및 5-HMF 함량 간의 상관관계도 높게 나타났다(p<0.01).

Keywords

References

  1. Kwon SK. Organosulfur compounds from Allium sativum and physiological activities. J. Appl. Pharmacol. 11: 8-32 (2003)
  2. Choi HK. A study on the antibacterial activity of garlic against Escherichia coli O157. J. Korean Practical Arts Edu. 14: 159-167 (2001)
  3. Ruffin J, Hunter SA. An evaluation of the effect of garlic as an antihypertensive agent. Ctyobios. 37: 85-89 (1983)
  4. Kim ES, Chun HJ. The anticarcinogenic effect of garlic juice against DMBA induced carcinoma on the hamster buccal pouch (in Korean). J. Korean Soc. Food Nutr. 22: 398-404 (1993)
  5. Belman S. Onion and garlic oils inhibit tumor promotion. Carcinogenesis 4: 1063-1067 (1983) https://doi.org/10.1093/carcin/4.8.1063
  6. Stoll A, Seebeck E, Chemical investigation on alliin, the specific principle of garlic. Adv. Enzymol. 11: 377-400 (1951)
  7. Chung KS, Kim JY, Kim YM. Comparison or antibacterial activities of garlic juice and heat-treated garlic juice. Korean J. Food Sci. Tcchnol. 35: 540-543 (2003)
  8. Kim DY, Kim KM, Hur CK, Kim ES, Cho IK, Kim KJ. Antimicrobial activity of garlic extracts according to different cooking methods. Korean J. Food Pres. 11: 400-404 (2004)
  9. Jo JS, Do JR, Koo JG. Pretreatment conditions of Porphyra yezoensis, Undaria pinnatifida and Laminaria religiosa for functional alage-tea. J. Korean Soc. Food Sci. Nutr. 27: 275-280 ( 1998)
  10. Choi Y, Lee SM, Chun J, Lee HB, Lee J. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem. 99: 381-387 (2006) https://doi.org/10.1016/j.foodchem.2005.08.004
  11. Yildiz F, Westhoff D. Associative growth of lactic acid bacteria in cabbage juice. J. Food Sci. 46: 962-963 (1981) https://doi.org/10.1111/j.1365-2621.1981.tb15397.x
  12. Woo KS. Effects of high temperature and pressure treatment on the physicochemical characteristics of licorice (Glycyrrhiza uralemsis Fisch). MS thesis, Chungbuk University, Cheongju, Korea (2005)
  13. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 26: 1199-1203 (1958)
  14. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. Antioxidative activity and physiological activity of some Korean medicinal plants. J. Korean. Food Sci. Technol. 27: 80-85 (1995)
  15. Dewnto V, Xianzhong W, Liu RH. Processed sweet corn has higher antioxidant activity. J. Agric. Food Chem. 50: 4959-4964 (2002) https://doi.org/10.1021/jf0255937
  16. Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559 (1999) https://doi.org/10.1016/S0308-8146(98)00102-2
  17. Bae SK, Lee YC, Kim HW. The browning reaction and inhibition of apple concentrated juice. J. Korean Soc. Food Sci. Nutr. 30: 6-13 (2001)
  18. Im KJ, Lee SK, Park DK, Rhee MS, Lee, JK. Effects of garlic extracts on the nitrosation. Agric. Chem. Biotechnol. 43: 110-115 (2000)
  19. Shin JH, Ju JC, Kwen OC, Yang SM, Lee SJ. Physicochemical and physiological activities of garlic from different area. Korean J. Food Nutr. 17: 237-245 (2004)
  20. Hong JJ, Ahn TH. Changes in total flavonoid and total polyphenol contents of leafy vegetables (spinach, chard and whorled mallow) by Blanching time. Korean J. Food Cookery Sci. 21: 190-194 (2005)
  21. Park GY, Lee SJ, Im JG. Effect of green tea catechin on cytochrome xanthine oxidase activities in liver and liver damage in streptozotochin induced diabetic rats. J. Korean Soc. Food Nutr. 26: 901-907 (1997)
  22. Renner E. Effect of differences in technology on the organoleptic and nutritive quality of UHT milk. Rivista della Societa Italiana di Scienza dell'Alimentazione 7: 499-504 (1978)
  23. Lee CK, Seo JM. Changes of the Constituents in the Rehmanniae Radix Preparata during processing. J. Korean Soc. Food Sci. Nutr. 33: 1748-1752 (2004) https://doi.org/10.3746/jkfn.2004.33.10.1748
  24. Kwak HM, Kim JY, Lim JH, Chung SK, Kwon SH, Jeong HH, Hur JM, Song KS. Changes in chemical composition and biological activities of oriental crude drugs by food processing techniques III-changes of HMF contents from roasted Asparagi tuber. Korean J. Pharmacogn. 36: 235-239 (2005)