Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Investigation of Antioxidative Activity and Stability of Ethanol Extracts of Licorice Root (Glycyrrhiza glabra)

감초의 에탄올 추출물의 항산화 활성 및 안정성 조사

  • Published : 2006.08.01
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Abstract

This study was carried out to optimize the conditions for the extraction of antioxidative materials from licorice root, Glycyrrhiz glabra. Chipped licorice roots were extracted with several solvents and their antioxidative activities were tested to determine the optimal extraction solvent. Among the solvents tested, 95% ethanol gave the highest free radical scavenging activity, and was therefore chosen as the optimal extracting solvent. The optimum extraction temperature and time were $20^{\circ}C$ and 12 hr, respectively. Next, the free radical scavenging activity of the ethanol extract was compared with that of other known antioxidants such as ${\alpha}-tocopherol$, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Ethanol extract of licorice root had greater antioxidative activity than ${\alpha}-tocopherol$ and a similar level to that of the two synthetic antioxidants (BHA and BHT). Moreover, the antioxidative activity of the ethanol extract was inhibited neither by heat treatment at $180^{\circ}C$ for 30 min nor by treatment at extreme pH. These findings suggest that ethanol extract of G. glabra may be useful as a natural antioxidant.

본 연구에서는 한방재료인 감초에서 식품의 보존력을 가지는 항산화성 유효 성분을 추출하기 위해 추출용매, 에탄올 농도, 온도, 시간 등의 여러 조건에서 추출실험을 수행하였다. 조제된 감초 에탄올 추출물을 이용하여 상용중인 항산화제와 항산화 활성을 비교하고 열과 pH 변화에 대한 안정성을 확인하여 천연 식품보존료로서의 이용 가능성을 검토하였다. 감초를 물, 메탄올, 에탄올, 아세톤, 에칠 아세테이트, 헥산 등의 용매로 추출하였을 때 항산화 활성은 극성이 높은 유기용매에서 높은 활성을 보였으며, 에탄올이 가장 적합한 것으로 판단되었다. 에탄올 농도별로 추출하였을 때는 무수에탄올일 때 항산화 활성이 가장 높았으며 95% 농도와는 유의한 차를 보이지 않아 추출 용매는 95% 에탄올로 선택하였다. 95% 에탄올로 감초를 추출할 때 은도와 시간은 항산화 활성에 큰 영향을 주지 않았다. 감초를 95%에탄올로 상온에서 12시간 추출하며 감압건조한 감초 에탄올 추출물은 천연 항산화제인 ${\alpha}-tocopherol$과 비교하여 free radical 소거능은 약하지만 유지산패 억제능력은 훨씬 더 좋았다. 감초 에탄올 추출물의 열과 pH처리에 따른 항산화 활성은 큰 현화를 보이지 않아 열과 pH에는 비교적 안정하다고 판단된다. 따라서 감초 에탄올 추출물은 항산화 활성도 뛰어날 뿐만 아니라 열과 pH에 대해서도 안정하여 천연보존료로서 사용이 가능하리라 사료된다.

Keywords

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