Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Induction of Quinone Reductase Activity in Hepatoma Cells by Paprika (Capsicum annuum L.)

파프리카 추출물이 quinone reductase 유도활성에 미치는 영향

  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Hyo-Jung (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Im, Hyo-Gwon (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, Syng-Ook (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (Department of Food Science and Technology, Keimyung University,The Center for Traditional Microorganism Resources, Keimyung University)
  • 유미희 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 이효정 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 임효권 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 이승욱 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터) ;
  • 이인선 (계명대학교 식품가공학,계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터)
  • Published : 2006.10.01
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Abstract

Phase 2 enzymes are transcriptionally induced by a wide variety of chemical agents and natural products, and their induction plays a critical role in protection against chemical carcinogens and other toxic xenobiotics. The activity of the methanol extract and fractions of paprika (Capsicum annuum L.) was examined in murine Hepa1c1c7 cells for the induction of nicotinamide adenine dinucleotide (phosphate) NAD(P)H/quinone reductase (QR). The ethyl acetate (EtOAc) fraction induced QR activity in a dose-dependent manner in the concentration range of 10 to $500\;{\mu}g/mL$ with a maximum of a 3.3-fold increase in induction. The EtOAc fraction also showed high QR induction potency in Ah-receptor-defective mutant of Hepa 1c1c7 cells ($BP^rcl$ cells), which indicates that this fraction is a monofunctional inducer of QR. These results suggest that useful cancer chemopreventive materials could be isolated from EtOAc fraction of Paprika.

Phase II 효소계는 자연계에 존재하는 다양한 화학물질과 천연 소재들에 의해 유도되며, 이들의 유도는 화학적 발암물질과 그 밖의 여러 가지 독성물질들로부터 생체를 보호하는데 중요한 역할을 한다. 본 연구에서는 파프리카의 메탄올 추출물을 제조하고, 이를 각 용매별로 분획하여 Hepa1c1c7 세포주를 이용하여 대표적인 암예방 효소인 quinone reductase의 유도 활성을 조사하였다. 그 중 QR 유도활성이 가장 높았던 EtOAc 분획물은 $10-500\;{\mu}g/mL$ 농도로 처리했을 때, $200\;{\mu}g/mL$의 농도에서 QR 활성이 약 3.3배로 가장 높게 나타났으며, 파프리카의 추출물의 총 폴리페놀 및 플라보노이드 함량 역시 EtOAc 분획물에서 가장 높게 나타났다. 또한 $BP^rcl$ 세포주에서도 농도 의존적으로 높은 QR 유도활성을 보여 파프리카의 EtOAc 분획물의 항암성분은 monofunctional inducer인 것으로 생각된다.

Keywords

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