Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Micropropagation of Hypericum erectum by Axillary Bud Culture

액아배양을 통한 고추나물의 대량증식

  • Jin, Mei-Lan (Department of Biology and Institute of plant Resources, Chonnam National University) ;
  • Nam, Dong-Woo (Department of Biology and Institute of plant Resources, Chonnam National University) ;
  • Ahn, Jun-Cheul (Department of Life Sciences, Seonam University) ;
  • Hwang, Baik (Department of Biology and Institute of plant Resources, Chonnam National University)
  • 김미란 (전남대학교 생물학과 및 자원식물연구소) ;
  • 남동우 (전남대학교 생물학과 및 자원식물연구소) ;
  • 안준철 (서남대학교 생명과학과) ;
  • 황백 (전남대학교 생물학과 및 자원식물연구소)
  • Published : 2006.02.28
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Abstract

One of micropropagation methods was investigated by using a multiple-shoots protocol. Multiple shoot formation was obtained from excised axillary buds of Hypericum erectum on half-strength or basal MS medium supplemented with TDZ or BA. The optimal combination of shoot multiplication for the production of more shoots with a suitable size was MS medium supplemented with $0.005\;mg{\cdot}L^{-1}$ TDZ (6.5 adventitious shoots per node). In vitro rooting was carried on half-strength MS medium with $1\;mg{\cdot}L^{-1}\;GA_3\;and\;0.5\;mg{\cdot}L^{-1}$ IBA treatment. In addition, the rooted cuttings were showed a better root growth in the greenhouse and survived in more than 90%. The results show that the species can be micropropagated effectively by the application of axillary bud culture systems.

기관배양을 통한 대량증식 방법의 일환으로 액아배양을 이용한 multiple shoot 유도 조건을 조사하였다. 기내 배양 중인 식물체로부터 액아를 절취하여 TDZ와 BA를 각각 농도 별로 첨가한 MS배지와 1/2 MS배지에서 6주간 배양한 결과, MS기본배지에 $0.005\;mg{\cdot}L^{-1}$ TDZ가 첨가된 처리구에서 효과가 가장 양호하였다. 한편 발생된 신초를 $1\;mg{\cdot}L^{-1}\;GA_3$와 $0.5\;mg{\cdot}L^{-1}$ IBA가 첨가된 1/2 MS배지에 배양했을 때 뿌리의 발생과 신장이 가장 양호하였다. 발근된 식물체를 포트에서 순화시켜 온실로 이식하였을 때 90%에 가까운 생존율을 보였으며 형태적인 변이 없이 생장하였다. 이상의 결과는 액아유도를 통한 대량번식의 가능성을 제시한다.

Keywords

References

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