Quantitative Analysis of Lignans in the Fruits of Acanthopanax Species by HPLC

  • Kim, Hye-Min (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Kim, Ju-Sun (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Lee, Sung-Jin (Gyeonggi Regional Research Center, Hankyong National University) ;
  • Lee, Gung-Pyo (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Cho, Seon-Haeng (Practical Arts, Gongju National University of Education) ;
  • Cheoi, Dae-Sung (Jeongseon Agricultural Technology & Extension Center)
  • Published : 2006.10.30

Abstract

High performance liquid chromatography (HPLC) was used for the analysis of the lignans eleutheroside B and eleutheroside E in the fruits of Acanthopanax species. A reverse-phase system using a gradient of $H_2O$ and acetonitrile as the mobile phase was developed and detection was at 210 nm. The analysis was successfully carried out within 20 min. The content of eleutheroside B and eleutheroside E in Acanthopanax species was measured in the fruits of A. senticosus (0.58 and $1.66\;{\mu}g/mg$, respectively), A. sessiliflorus (1.15 and $8.49\;{\mu}g/mg$, respectively), A. koreanum (2.16 and $1.80\;{\mu}g/mg$, respectively), and A. divaricatus (1.06 and $7.08\;{\mu}g/mg$, respectively).

Keywords

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