Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effect of Diet with Meat for Crossbred Pig Fed with Tangerine Peel on lipid Metabolism, Protein Level and Enzyme Activities in Rats

감귤피를 급여한 교잡종 돼지 고기가 흰쥐의 지질대사, 단백질 농도 및 효소 활성에 미치는 영향

  • Moon Yoon-Hee (Department of Food Science and Technology, Kyungsung University) ;
  • Yang Seung-Joo (Jejudo Provincial Government) ;
  • Jung In-Chul (Division of Food Beverage and Culinary Arts, Daegu Technical College) ;
  • Yang Yang-Han (Department of Food Science and Nutrition, Cheju National University) ;
  • Koh Jin-Bog (Department of Life Science, Silla University)
  • Published : 2006.03.01
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Abstract

Three different diet were made with meat for Crossbred pig ($^{\circ}_{+}$, 195 days old, $l15{\pm}5\;kg,\;Landrace{\times}Yorkshire{\times}Duroc$) and used as feed for rats after transformed into 12% protein, These diets include a pork not fed with tangerine peel during finishing period (Control, $T_0$), a pork fed with 3 and 5% tangerine peel during growing and finishing period, respectively ($T_1$), and a pork fed with 6 and 10% tangerine peel during growing and finishing period respectively($T_2$). The effects of the diet on physiological activities of rats were studied by feeding 17 weeks-old Sprague-Dawley rats with the three diets for 4 weeks. The feed intake, weight gain, feed efficiency ratio, and weight of liver, kidney, spleen and epididymal fat pad for the rats was similar among the diets. The total lipid level of liver was similar among $T_0,\;T_1\;and\;T_2$. However, it was found that $T_1\;and\;T_2$ had significantly lower triglyceride and cholesterol level than $T_0$(p<0.05). All the diet groups showed similar trends in terms of the serum total lipid, phospholipid, triglyceride, total cholesterol, HDL-cholesterol and LDL-cholesterol level, and atherogenic index, hemoglobin level, and ${\gamma}-GTP$, ALT, AST and ALP activities.

감귤피를 급여하지 않은 교잡종 돼지고기(대조구, $T_0$), 육성기와 비육기에 각각 3% 및 5% 급여한 돼지고기($T_1$), 그리고 6% 및 10%를 급여한 돼지고기($T_2$)등 3 종류를 각각 단백질로 환산하여 12% 되도록 흰쥐 식이에 첨가하였다. 이식이를 생후 17주령의 흰쥐에 4주간 급여하여 영양대사에 미치는 영향을 조사하였다. 대조구($T_0$)와 감귤피 급여구($T_1$과 $T_2$)의 사이에 흰쥐의 증체량, 식이섭취량, 식이효율, 장기(간, 신장, 비장, 부고환 지방)무게는 유의적 차이를 보이지 않았다. 혈청의 지질(총지질, 인지질, 중성지질, 총 콜레스테롤, HDL-콜레스테롤, LDL-콜레스테롤), 동맥경화지수, 혈당, 혈색소 함량 및 효소(${\gamma}-GTP$, ALT, AST, ALP) 활성은 모두 유의적 차이를 보이지 않았다. 흰쥐의 간에서 얻은 총지질 함량은 유의적 차이를 보이지 않았으나, 중성지질과 콜레스테롤 함량이 대조구보다 감귤피 급여구가 낮은 값을 보여(p<0.05), 감귤피 첨가 사료로 사육한 교잡종 돼지고기가 흰쥐 간의 중성 지질 및 콜레스테롤을 감소시키는 효과가 있는 것으로 나타났다.

Keywords

References

  1. Braddock, R. J. (1983) Utilization of citrus juice vesicle and peel fiber. Food Tech. 12, 85-89
  2. Braddock, R. J. and Crandall, P. G. (1981) Carbohydrate fiber from orange albedo. J. Food Sci. 46, 650-655 https://doi.org/10.1111/j.1365-2621.1981.tb04936.x
  3. Chung, S. K., Kim, S. H., Choi, Y. H., Song, E. Y., and Kim, S. H. (2000) Status of citrus fruit production and view of utilization in Cheju. Food Ind. Nutr. 5, 42-52
  4. Flora, S. J., Singh, S., and Tomdon, S. K. (1985) Combined effects of thiamin and calcium disodium ver­senate on lead toxicity. Life Science, 38, 67-69 https://doi.org/10.1016/0024-3205(86)90276-6
  5. Folch, J., Lees, M., and Stanley, G. S. H. (1957) A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem. 226, 497-509
  6. Frings, C. S. and Dunn, R. T. (1970) A colorimetric method for determination of total serum lipid based on the sulfophospho-vanillin reaction. Am. J. Clin Path. 53, 89-91 https://doi.org/10.1093/ajcp/53.1.89
  7. Haglund, O., Loustarinen, R., Wallin, R., Wibell, I., and Saldeen, T. (1991) The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur. J. Nutr. 121, 165-172 https://doi.org/10.1093/jn/121.2.165
  8. Jeong, S. M., Kim, S. Y., Park, H. R., and Lee, S. C. (2004) Effect of far-infrared radiation on the antioxidant activity of extracts from cirrus unshiu peels. J. Kor. Soc Food Sci. Nutr. 33, 1580-1583 https://doi.org/10.3746/jkfn.2004.33.9.1580
  9. Joo, S. T., Hur, S. J., Lee, J. I., Lee, J. R., Kim, D. H., Ha, Y. R., and Park, G. B.(1999) Influence of dietary onion peel on lipid oxidation, blood characteristics and antimutagenicity of pork during storage. Kor. J. Anim. Sci. 41, 671-678
  10. Kailash, P. (1999) Reduction of serum cholesterol and hypercholesterolemic atherosclerosis in rabbits by secoi­solariciresinol diglucoside isolated from flaxseed. Circu­lation 99, 1355-1362 https://doi.org/10.1161/01.CIR.99.10.1355
  11. Kang, B. H., Son, H. Y, Ha, C. S., Lee, H. S., and Song, S. W. (1995) Reference values of hematology and serum chemistry in Ktc: Sprague-Dawley rats. Kor. J. Lab. Ani. Sci. 11, 141-145
  12. Kim, H. J., Bae, K. H., Lee, H. J., Eun, J. B., and Kim, M. K. (1999) Effect of hesperidin extracted from tan­gerine peel on cd and lipid metabolism and antioxidative capacity in rats. Korean J. Nutr. 32, 137-149
  13. Kim, H. Y, Song, S. W., Ha, C. S., and Han, S. S. (1993) Effects of the population density on growth and various physiological values of Sprague-Dawley rats. Kor. J. Lab. Ani. Sci. 9, 71-82
  14. Kim, S. M. (2001) Natural resources and functional meat products. Food Industry and Nutr. 6, 46-53
  15. Kinosian, B., Glick, H., Preiss, L., and Puder, K. I. (1995) Cholesterol and coronary heart disease: predicting risk in men by changes in levels and ratios. J. Invest Med. 43, 443-450
  16. Koh, J. B. (2002) Effect of Cordyceps militaris on lipid metabolism, protein levels and enzyme activities in rats fed high fat diet. Korean J. Nutr. 35, 414-420
  17. Koh, J. B. and Choi, M. A. (2001) Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J. Nutr. 34, 265-270
  18. Koh, J. B. and Choi, M. A. (2003) Effect of Paecilomyes japonica on lipid metabolism in rats fed high fat diet. J. Kor. Soc. Food Sci. Nutr. 32, 238-243 https://doi.org/10.3746/jkfn.2003.32.2.238
  19. Koh, J. B. and Choi, M. A. (2004) Effects of liquid culture of Coriolus versicolor on lipid metabolism, protein level and enzyme activities in rats. J. Kor. Soc. Food Sci. Nutr. 33, 512-517 https://doi.org/10.3746/jkfn.2004.33.3.512
  20. Koh, J. B., Kim, J. Y., Jung, I. C., Yang, S. J. and Moon, Y. H. (2006) Effect of diet with meat for crossbred black pig fed with tangerine peel on lipid metabolism, protein level and enzyme activities in rats. Journal of Life Science 16, in press
  21. Lanza, M., Fasone, V., Galofaro, V., Barbagallo, D., Bella, M., and Pennisi, P. (2004) Citrus pulp as an ingredient in ostrich diet: effects on meat quality. Meat Science, 68, 269-275 https://doi.org/10.1016/j.meatsci.2004.03.005
  22. Lee, H. J. and Kim, M. K. (1998) Retarding effect of dietary fibers isolated from persimmom peels and jujubes on in vitro glucose, bile acid, and cadmium transport. Korean J. Nutr. 31, 809-822
  23. Lee, J. I., Joo, S. T., and Park, G. B. (1999) The effect of fish powder oil additive in diet on the fatty acids composition and cholesterol content of pork belly from the pigs fed on the fermented sawdust feds. Kor. J. Food Sci. Ani. Resour. 19, 127-135
  24. Park, B. S. (2004) Effect of the feeding belly fat on plasma lipid levels in rats. Kor. J. Food Sci. Ani. Resour. 24, 189-197
  25. SAS (1988) SAS/STAT User's Guide. Release 6.03 edition, SAS Institute, Inc., Cary, NC, USA
  26. Schaefer, E. J. (1995) Lipoproteins, nutrition, aging and atherosclerosis. Am. J. Clin Nutr. 61, 726-728 https://doi.org/10.1093/ajcn/61.3.726S
  27. Sohn, J. S. and Kim, M. K. (1998) Effects of hesperidin and naringin on antioxidative capacity in the rat. Korean J. Nutr. 31, 687-696
  28. Wolford, S. T., Schroer, R. A, and Gohs, F. X. (1986) Reference database for serum chemistry and hematology values in laboratory animals. J. Toxicology and Environmental Health 18, 161-188 https://doi.org/10.1080/15287398609530859
  29. Yang, S. J., Song, J. Y., Yang, T. J., Jung, J. C., Park, K. S., and Moon, Y. H. (2005) Effect of feeding of unshiu orange bypro ducts on nutritional composition and palata­bility of crossbred pork loin. J. Kor. Sos. Food Sci. Nutr. 34, 1593-1598 https://doi.org/10.3746/jkfn.2005.34.10.1593