Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Effect of Alpha-tocopherol Supplementation on the Improvement of Antioxidant Status and Lymphocyte DNA Damage in Postmenopausal Women

비타민 E 보충섭취가 폐경기 여성의 혈장 항산화 영양상태 및 DNA 손상 개선에 미치는 영향

  • Kim, Chang-Suk (Department of Food & Nutrition, Daedeok Valley Campus, Hannam University) ;
  • Kang, Hae-Jin (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Soon-Hee (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Yoo-Kyoung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kang, Myung-Hee (Department of Food & Nutrition, Daedeok Valley Campus, Hannam University)
  • 김창숙 (한남대학교 생명나노과학대학 식품영양학과) ;
  • 강해진 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이순희 (경희대학교 동서의학대학원 의학영양학과) ;
  • 박유경 (경희대학교 동서의학대학원 의학영양학과) ;
  • 강명희 (한남대학교 생명나노과학대학 식품영양학과)
  • Published : 2007.12.30
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Abstract

The purpose of this project was to evaluate whether vitamin E supplementation could improve the antioxidant status and lymphocyte DNA damage in Korean postmenopausal women. This was double blinded, placebo-controlled trial. Thirty-five subject were randomized to receive either placebo 400 mg/capsule or natural $\alpha$-tocopherol 400 IU/capsule, 2 times a day for 6 weeks. We measured plasma vitamin C, $\alpha$-tocopherol, $\gamma$-tocopherol, $\alpha$-carotenoid, $\beta$-carotenoid, lycopene concentration and tail length, %DNA in tail, tail moment in lymphocyte DNA damage index. Vitamin E supplementation group had significantly increased plasma vitamin C(p<0.05), $\alpha$-tocopherol(p<0.000), whereas $\gamma$-tocopherol(p<0.000) and tail length(p<0.05) were significantly decreased. However, placebo supplementation group also had significantly increased plasma vitamin C(p<0.05). In conclusion, our study shows that vitamin E supplementation to Korean postmenopausal women may partially improve antioxidant status and lymphocyte DNA damage.

본 연구는 비타민 E의 보충섭취가 폐경기 여성의 항산화 영양상태 및 임파구 DNA 손상을 개선할 수 있는지를 알아보고자 수행되었으며, 이중맹검법으로 실시되었고 플라시보를 위약군으로 하였다. 폐경기 여성 35명을 대상으로 6주 동안 비타민 E 보충섭취군에게는 비타민 E 캡슐($\alpha$-tocopherol 성분 400 IU/capsule)을, 위약군에게는 대두유로 만든 위약 캡슐(400 mg/capsule)을 하루 2회 섭취하도록 하였으며, 이 때 두 군의 분류는 무작위로 하였다. 항산화 영양상태를 알아보기 위해 혈장 vitamin C, $\alpha$-tocopherol, $\gamma$-tocopherol, $\alpha$-carotenoid, $\beta$-carotenoid, lycopene 농도를 측정하였고, 임파구 DNA 손상정도를 알아보기 위해서는 Comet assay를 이용하여 tail length, %DNA in tail, tail moment를 측정하였다. 비타민 E 보충섭취군에서는 혈장 vitamin C(p<0.05)와 $\alpha$-tocopherol(p<0.000)농도가 유의적으로 증가하였고, $\gamma$-tocopherol(p<0.000) 농도와 tail length(p<0.05)는 유의적으로 감소하였다. 반면, 위약군에서는 혈장 vitamin C(p<0.05)의 농도만이 유의적으로 증가하였다. 결론적으로, 본 연구는 비타민 E의 보충섭취가 혈장 항산화 상태 및 DNA 손상에 대해 부분적인 개선 효과가 있음을 보여주었다.

Keywords

References

  1. Benzi IF. Evolution of antioxidant defence mechanisms. Eur J Nutr 2000; 39: 53-61 https://doi.org/10.1007/s003940070030
  2. Stampfer MJ, Hennekens CH, Manson JE, Colditz GA, Rosner B, Willett WC. Vitamin E consumption and the risk of coronary disease in women. N Engl J Med 1993; 328: 1444-1449 https://doi.org/10.1056/NEJM199305203282003
  3. Jung KA, Kim SY, Choi YJ, Woo JI, Chang YK. The Nutritional Status of Antioxidant Vitamins in Relation to Serum MDA Level in Postmenopausal Women. Korean J Nutr 2001; 34(3): 330-337
  4. Kim SY, Jung KA. Correlation of the Nutritional Status of Antioxidant Vitamins and Serum Lipids and MDA Levels in Postmenopausal Women. J East Asian Soc Dietary Life 2006; 16 (1): 145-155
  5. Stephens NG, Parsons A, Schofield PM, Kelly F, Cheeseman K, Mitchinson MJ. Randomised controlled trial of vitamin E in patients with coronary disease: Cambridge Heart Antioxidant Study (CHAOS). Lancet 1996; 23: 347(9004): 781-786 https://doi.org/10.1016/S0140-6736(02)96798-X
  6. ATBC (alpha-tocopherol, beta-carotene) Cancer Prevention Study Group. The effect of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smokers. N Engl J Med 1994; 330: 1029-1035 https://doi.org/10.1056/NEJM199404143301501
  7. GISSI-Prevention Investigators. Dietary supplementation with n-3 polyun- saturated fatty acids and vitamin E after myocardial infraction; Results of the GISSI-Prevention Trial. Lancet 1999; 354: 447-455 https://doi.org/10.1016/S0140-6736(99)07072-5
  8. HOPE Study Investigators. Vitamin E supplementation and cardiovascular events in high-risk patients. N Engl J Med 2000; 342: 154-160 https://doi.org/10.1056/NEJM200001203420302
  9. Salonen JT, Nyyssonen K, Salonen R, Lakka HM, Kaikkonen J, Porkkala-Sarataho E, Voutilainen S, Lakka TA, Rissanen T, Leskinen L. Antioxidant Supplementation in Atherosclerotic Prevention (ASAP) study: a randomized trial of the effect of vitamins E and C on 3-year progression of carotid atherosclerosis. J Intern Med 2002; 248 (5) : 377-386
  10. McGavin JK, Mann JI, Skeaff CM, Chisholm A. Comparison of a vitamin E-rich diet and supplemental vitamin E on measures of vitamin E status and lipoprotein profile. Eur J Clin Nutr 2001; 55: 555-561 https://doi.org/10.1038/sj.ejcn.1601182
  11. Arrol S, Mackness MI, Durrington PN. Vitamin E supplementation increases the resistance of both LDL and HDL to oxidation and increases cholesteryl ester transfer activity. Atherosclerosis 2000; 150(1): 129-134 https://doi.org/10.1016/S0021-9150(99)00353-6
  12. McAnulty SR, McAnulty LS, Nieman DC, Morrow JD, Shooter LA, Holmes S, Heward C, Henson DA. Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise. J Nutr Biochem 2005; 16: 530-537 https://doi.org/10.1016/j.jnutbio.2005.02.001
  13. Kim WK, Kim HY, Kim MJ, Kim SH. Effects of Vitamin E Supplementation on Antioxidant Status and Immune Responses in Female Athletes. Korean J Nutr 1999; 32(7): 781-786
  14. Oh BS. The Effect of Antioxidants Supplementation on Cell Membrane Damage Index (LDH, MDA) and Anti-Inflammatory Immunity Reaction Substance (IL-1, IL-2) in Middle Long Distance Runners of Track and Field. Korean J Physi Educat 1999; 38(4): 387-400
  15. Kim WK. Originals: Effects of Vitamin E Supplementation on Immune Response and Antioxidant defense Parameters in Healthy Korean Elderly Women. J Korean Soc Food Sci Nutr 1999; 28 (4) : 924-933
  16. Huang HY, Helzlsouer KJ, Appel LJ. The effects of vitamin C and vitamin E on oxidative DNA damage: results from a randomized controlled trial. Cancer Epidemiol Biomarkers Prev 2000; 9(7): 647-652
  17. Kan E, Undeger U, Bali M, Basaran N. Assessment of DNA strand breakage by the alkaline COMET assay in dialysis patients and the role of Vitamin E supplementation. Mutat Res 2002; 520(1-2): 151-159
  18. Brennan LA, Morris GM, Wasson GR, Hannigan BM, Barnett YA. The effect of vitamin C or vitamin E supplementation on basal and $H_2O_2$-induced DNA damage in human lymphocytes. Br J Nutr 2000; 84 (2): 195-202 https://doi.org/10.1079/096582197388680
  19. Oscagli E, Sardas S, Biri A. Assessment of DNA damage in postmenopausal women under hormone replacement therapy. Maturitas 2005; 51: 280-285 https://doi.org/10.1016/j.maturitas.2004.08.010
  20. Park YK, Kim YA, Park EJ, Kang MH. Estimated carotenoids intake in korean adults using food-frequency questionnaire association with smoking, drinking and other life-style factors. Nutr Sci 2001; 4 (2) : 98-103
  21. Park YK, Kim YA, Park EJ, Kim JS, Kang MH. Estimated Flavonoids Intake in Korean Adults Using Semiquantitative Food frequency Questionnaire. Korean J Nutr 2002; 35 (10): 1081-1088
  22. Kang MH, Yun JS. The Effects of Exercise on the Vitamin C and E Intakes and Their Plasma Levels of Vitamin C, a -tocopherol and r -tocopherol in Young Male Adults. Korean J Nutr 2001; 34 (3): 306-312
  23. Cooney RV, Franke AA, Harwood PJ, Hatch-Pigott V, Custer LJ, Mordan LJ. Gamma-tocopherol detoxification of nitrogen dioxide superiority to alpha-tocopherol. Proc Natl Acad Sci USA 1993: 90(5): 1771-1775
  24. Ble-Castillo JL, Carmona-Diaz E, Mendez JD, Larios-Medina FJ, Medina-Santillan R, Cleva-Villanueva G, Diaz-Zagoya JC. Effect of ${\alpha}-tocopherol$ on the metabolic control and oxidative stress in female type 2 diabetics. Biomedicine & Pharmacotherapy 2005; 59: 290-295 https://doi.org/10.1016/j.biopha.2005.05.002
  25. Naziroglu M, Simsek M, Simsek H, Aydilek N, Ozcan Z, Atilgan R. The effects of hormone replacement therapy combined with vitamins C and E on antioxidants levels and lipid profiles in postmenopausal women with Type 2 diabetes. Clin Chim Acta 2004; 344: 63-71 https://doi.org/10.1016/j.cccn.2004.01.031
  26. Huang HY, Caballero B, Chang S, Alberg A, Semba R, Schneyer C, Wilson RF, Cheng TY, Prokopowicz G, Barnes GJ 2nd, Vassy J, Bass EB. Multivitamin/mineral supplements and prevention of chronic disease. Evid Rep Technol Assess (Full Rep) 2006; 139: 1-117
  27. Hosomi A, Arita M, Sato Y, et al. Affinity for alpha-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs. FEBS Lett 1997; 409 (1): 105-108 https://doi.org/10.1016/S0014-5793(97)00499-7
  28. Swanson JE, Ben RN, Burton GW, Parker RS. Urinary excretion of 2,7,8-trimethyl-2- (beta-carboxyl) -6-hydroxychroman is a major route of elimination of gamma-tocopherol in humans. J Lipid Res 1999; 40 (4) : 665-671
  29. Jiang Q, Elson-Schwab I, Courtemanche C, Ames BN. Gammatocopherol and its major metabolite, in contrast to alhpa-tocopherol, inhibit cyclooxygenase activity in macrophages and epithelial cells. Proc Natl Acad Sci USA 2000; 97 (21): 11494-11499
  30. Huang HY, Appel LJ. Supplementation of diets with alpha-tocopherol reduces serum concentrations of gamma- and delta-tocopherol inhumans. J Nutr 2003; 13 (10): 3137-3140
  31. Kontush A, Finckh B, Karten B, Kohlschutter A, Beisiegel U. Antioxidant and prooxidant activity of ${\alpha}-tocopherol$ in human plasma and low density lipoprotein. J Lipid Res 1996; 37: 1436-1448
  32. Saldeen T, Li D, Mehta JL. Differential effects of alpha- and gamma-tocopherol on low density lipoprotein oxidation, superoxide activity, platelet aggregation and arterial thrombogenesis. J Am Coll Cardiol 1999; 34: 1208-1215 https://doi.org/10.1016/S0735-1097(99)00333-2
  33. Lim JY, Kim OH, Kim JH. Effects of Antioxidant Supplementation on Lipid Profiles in Elderly Women. Korean J Comm Nutr 2006; 11 (1): 133-142
  34. Park SM, Yu JG, Lee JY. Analysis of Factors to Influence Requirements of Vitamin E and Vitamin C in Young and Healthy Men and Women. Korean J Nutr 1998; 31 (4): 729-738
  35. Jeon EJ, Park YK, Kim JS, Kang MH. Comparison of the Protective Effect of Antioxidant Vitamins and Fruits or Vegetable Juices on DNA Damage in Human Lymphocyte Cells Using the Comet Assay. Korean J Nutr 2004; 37 (6): 440-447
  36. Park EJ, Kim JS, Jeon EJ, Kim HY, Park YK, Kang MH. The Effects of Purple Grape Juice Supplementation on improvement of Antioxidant Status and Lymphocyte DNA Damage in Korean Smokers. Korean J Nutr 2004; 37 (4): 281-290
  37. Kim HY, Park YK, Kim TS, Kang MH. The Effect of Green Vegetable Drink Supplementation on Cellular DNA Damage and Antioxidant Status of Korean Smokers. Korean J Nutr 2006; 39 (1): 18-27
  38. Kim JS, Kim HY, Park YK, Kim TS, Kang MH. The Effects of Green Vegetable Juice (Angelica Keiskei) Supplementation on Plasma Lipids and Antioxidant Status in Smokers. Korean J Nutr 2003; 36 (9) : 933-941
  39. Kim HA, Song KH. Comparison of Attitudinal Beliefs regarding Smoking and Antioxidant Vitamins Status in the College Male Smokers and Non-smokers. Korean J Food Culture 2002: 17 (3): 329-336
  40. Kim KJ, Lee HJ, Park YK, Kang MH. Association between Plasma Tocopherol Levels and Related Factors in Middle-Aged Korean Men. Korean J Nutr 2006; 39 (8): 1-13
  41. Traber MG, Sokol RJ, Kohlschutter A, Yokota T, Muller DP, Dufour R, Kayden HJ. Impaired discrimination between stereoisomers of a -tocopherol in patients with familial isolated vitamin E deficiency. J Lipid Res 1993; 34: 201-210
  42. Rasool AH, Rehman A, Wan Yusuf WN, Rahman AR. Vitamin E and its effect on arterial stiffness in postmenopausal women- a randomized controlled trial. Int J Clin Pharmacol Ther 2003; 41(12): 587-592 https://doi.org/10.5414/CPP41587
  43. The Korean Nutrition Society. Dietary Reference Intakes for Koreans (KDRIs). Seoul: KookJin Pub; 2005. p.98-104