Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antiasthmic Effect of Fermented Artemisia princeps in Asthmic Mice Induced by Ovalbumin

  • Bae, Eun-Ah (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Sciences, Kyung Hee University) ;
  • Min, Sung-Won (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Sciences, Kyung Hee University) ;
  • Lee, Bo-Mi (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Sciences, Kyung Hee University) ;
  • Kim, Nam-Jae (Korea Herbal Research Institute, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and PMRC, Kyung Hee University) ;
  • Han, Eun-Joo (Ganghwa Agricultural R&D Center) ;
  • Chung, Hae-Gon (Ganghwa Agricultural R&D Center) ;
  • Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Sciences, Kyung Hee University)
  • Published : 2007.09.30
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Abstract

Artemisia princeps Pampanini (AP) was fermented with Bifidobacterium infantis K-525 and its antiasthmic effect investigated. AP and fennented AP (FAP) reduced the IgE level in the blood of ovalbumin-induced asthmic mice. Moreover, FAP reduced the IgE, proinflammatory cytokine IL-6, and IL-4 levels in the trachea, as well as in the lung of the experimental asthmic mice, whereas AP only reduced the IgE and IL-6 levels in the lungs. Nonetheless, AP and FAP both inhibited the mRNA expression of IL-6 and TNF-${\alpha}$ in IgE-induced RBL-2H3 cells. The in vivo antiasthmic effect of FAP was more potent than that of AP. Therefore, these findings suggest that the enhanced antiasthmic effect of AP after bifidus fermentation was possibly due to the regulation of the proinflammatory cytokine biosynthesis of IL-6 and TNF-${\alpha}$.

Keywords

References

  1. Bielory, L. 2004. Complementary and alternative interventions in asthma, allergy, and immunology. Ann. Allergy Asthma Immunol. 93(2 Suppl. 1): S45-S54 https://doi.org/10.1016/S1081-1206(10)61486-X
  2. Bruhns, P., S. Fremont, and M. Daeron. 2005. Regulation of allergy by Fc receptors. Curr. Opin. Immunol. 17: 662-669 https://doi.org/10.1016/j.coi.2005.09.012
  3. Ha, C.-G., J.-K. Cho, C.-H. Lee, Y.-G. Chai, Y.-A. Ha, and S.-H. Shin. 2006. Cholesterol lowering effect of Lactobacillus plantarum isolated from human feces. J. Microbiol. Biotechnol. 16: 1201-1209
  4. Hur, H. J., K. W. Lee, H. Y. Kim, D. K. Chung, and H. J. Lee. 2006. In vitro immunopotentiating activities of cellular fractions of lactic acid bacteria isolated from kimchi and bifidobacteria. J. Microbiol. Biotechnol. 16: 661-666
  5. Kim, S. H., S. D. Lee, W. B. Kim, M. G. Lee, and N. D. Kim. 1997. Determination of a new antiulcer agent, eupatilin, in rat plasma, bile, urine, liver homogenate by high performance liquid chromatography. Res. Commun. Mol. Pathol. Pharmacol. 97: 165-170
  6. Kim, D. H., H. K. Na, T. Y. Oh, W. B. Kim, and Y. J. Surh. 2004. Eupatilin, a pharmacologically active flavone derived from Artemisia plants, induces cell cycle arrest in rastransformed human mammary epithelial cells. Biochem. Pharmacol. 68: 1081-1087 https://doi.org/10.1016/j.bcp.2004.04.027
  7. Kim, J. Y., E. Y. Kwon, Y. S. Lee, W. B. Kim, and J. Y. Ro. 2005. Eupatilin blocks mediator release via tyrosine kinase inhibition in activated guinea pig lung mast cells. J. Toxicol. Environ. Health A 68: 2063-2080 https://doi.org/10.1080/15287390500177024
  8. Kim, J. Y., D. Y. Kim, Y. S. Lee, B. K. Lee, K. H. Lee, and J. Y. Ro. 2006. DA-9601, Artemisia asiatica herbal extract, ameliorates airway inflammation of allergic asthma in mice. Mol. Cells 22: 104-112
  9. Mastuda, H., T. Morikawa, K. Ueda, H. Managi, and M. Yoshikawa. 2002. Structural requirement of flavonoids for inhibition of antigen-induced degranulation, TNF-$\alpha$ and IL- 4 production from RNL-2H3 cells. Bioorg. Med. Chem. 10: 2123-2128
  10. Moscatelli, V., O. Hnatyszyn, C. Acevedo, J. Megias, M. J. Alcaraz, and G. Ferraro. 2006. Flavonoids from Artemisia copa with anti-inflammatory activity. Planta Med. 72: 72-74 https://doi.org/10.1055/s-2005-873177
  11. Plaut, M., J. H. Pierce, C. Whatson, J. Hanley-Hyde, R. P. Nordan, and W. E. Paul. 1989. Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophore. Nature 339: 64-67 https://doi.org/10.1038/339064a0
  12. Park, S. C., J. H. Yoon, W. Kim, G. Y. Gwak, K. M. Kim, S. H. Lee, S. M. Lee, and H. S. Lee. 2006. Eupatilin attenuates bile acid-induced hepatocyte apoptosis. J. Gastroenterol. 41: 772-778 https://doi.org/10.1007/s00535-006-1854-6
  13. Ryu, S. N., S. S. Han, J. J. Yang, H. G. Jeong, and S. S. Kang. 2005. Variation of eupatilin and jaceosidin content of mugwort. Korean J. Crop Sci. 50(Suppl.): 204-207
  14. Sakuma, S., Y. Higashi, N. Sato, T. Sasakawa, T. Sengoku, Y. Ohkubo, T. Amaya, and T. Goto. 2001. Tacrolimus suppressed the production of cytokines involved in atopic dermatitis by direct stimulation of human PBMC system (comparison with steroids). Int. Immunopharmacol. 1: 1219-1226 https://doi.org/10.1016/S1567-5769(01)00059-5
  15. Schafer-Korting, M., M. H. Schmid, and H. C. Korting. 1996. Topical glucocorticoids with improved risk-benefit ratio. Drug Safety 14: 375-385 https://doi.org/10.2165/00002018-199614060-00003
  16. Seo, H. J. and Y. J. Surh. 2001. Eupatilin, a pharmacologically active flavone derived from Artemisia plants, induces apoptosis in human promyelocytic leukemia cells. Mutat. Res. 496: 191-198 https://doi.org/10.1016/S1383-5718(01)00234-0
  17. Shin, Y. W., E. A. Bae, S. S. Kim, Y. C. Lee, and D. H. Kim. 2005. Effect of ginsenoside Rb1 and compound K in chronic oxazolone-induced mouse dermatitis. Int. Immunopharmacol. 5: 1183-1191 https://doi.org/10.1016/j.intimp.2005.02.016
  18. Shin, T. W., E. A. Bae, B. Lee, S. Min, J. H. Lee, N. I. Baek, S. N. Ryu, H. G. Chung, and D. H. Kim. 2006. Effect of fermented lactic acid bacteria on antiallergic effect of Artemisia princeps Pampanini. J. Microbiol. Biotechnol. 16: 1464-1467
  19. Simons, F. E. R. 1992. The antiallergic effects of antihistamines (H1-receptor antagonists). J. Allergy Clin. Immunol. 90: 705-715 https://doi.org/10.1016/0091-6749(92)90156-V
  20. Stevens, R. L. and K. F. Austen. 1989. Recent advances in the cellular and molecular biology of mast cells. Immunol. Today 10: 381-386 https://doi.org/10.1016/0167-5699(89)90272-7
  21. Wuthrich, B. 1989. Epidemiology of the allergic diseases: Are they really on the increase? Int. Arch. Allergy Appl. Immunol. 90(Suppl. 1): 3-10 https://doi.org/10.1159/000235067