Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Investigation of Antioxidative and Tryosinase Inhibitory Activities of the Seed Extracts

종자 추출물의 항산화 활성 및 Tyrosinase 저해 활성 탐색

  • Jeong, Jin-A (Dept of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kwon, Su-Hyun (Dept of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Young-Jung (Dept of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Shin, Chang-Seob (Scientific Forest, College of Agricultlue, Life & Environment Sciences, Chungbuk National University) ;
  • Lee, Cheol-Hee (Dept of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University)
  • 정진아 (충북대학교 원예과학과 & 생물건강산업개발연구센터) ;
  • 권수현 (충북대학교 원예과학과 & 생물건강산업개발연구센터) ;
  • 김영중 (충북대학교 원예과학과 & 생물건강산업개발연구센터) ;
  • 신창섭 (충북대학교 농업생명환경대학 부속학술림) ;
  • 이철희 (충북대학교 원예과학과 & 생물건강산업개발연구센터)
  • Published : 2007.04.30
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Abstract

Bioactive substances, antioxidant activities and tyrosinase inhibitory activities of seed extracts were evaluated to discover new functional materials, using 13 edible or medicinal plants. Total polyphenol and flavonoid contents were highest in Taxus cuspidata, with $57.51mg{\cdot}g^{-1}\;and\;7.98mg{\cdot}g^{-1}$, respectively. Seed extract of Vitis coignetiae${\times}$Vitis labruscana($RC_{30}=0.030mg{\cdot}ml^{-1}$) was found to be the most effective in DPPH radical scavenging test, and the highest ABTS radical scavenging activity was shown in Diospyros lotus($RC_{30}=0.044mg{\cdot}ml^{-1}$). Inhibitory effects on peroxidation of linoleic acid determined by ferric thiocyanate (FTC) method were similar to BHT in Diospyros kaki, Diospyros lotus, Magnolia officinalis, Styrax obassia, Vitis coignetiae, Vitis coignetiae${\times}$Vistis labruscana, Zizyphus jujuba, Zizyphus jujuba var. inermis. For the inhibition against mushroom tyrosinase, Diospyros kaki, Diospyros lotus, Poncirus tritoliata, Prunus serrulata var. spomtanea, Zizyphus jujuba and Zizyphus jujuba var. inermis exhibited inhibitory activity, and especially Diospyros lotus showed the strongest inhibition.

새로운 기능성 소재를 탐색하기 위하여, 13종의 식용 및 약용식물의 종자추출물을 조제하여 생리활성물질 함량, 항산화활성 그리고 tyrosinase 억제활성을 분석하였다. 총 폴리페놀 및 플라보노이드 함량은 주목에서 각각 $57.51mg{\cdot}g^{-1}$과 $7.98mg{\cdot}g^{-1}$으로 가장 높게 나타났다. 종자추출물의 DPPH 라디칼 소거 활성은 머루$\times$'캠벨얼리' 교배종에서 $RC_{50}$이 $0.03mg{\cdot}ml^{-1}$로 가장 높았고, ABTS 라디칼 소거활성은 고음나무 종자에서 $RC_{50}$이 $0.109mg{\cdot}ml^{-1}$로 가장 높게 나타났다. Ferric thiocyanate(FTC) 방법으로 linoleic acid에 대한 과산화 억제효과를 조사한 결과, 감나무, 고욤나무, 당후박나무, 쪽동백나무, 머루, 머루$\times$'캠벨얼리' 교배종, 묏대추나무, 대추나무의 종자추출물에서 BHT와 유사한 수준의 억제활성을 보였다. 종자추출물의 tyrosinase 억제활성은 감나무, 고욤나무, 탱자나무, 벚나무, 묏대추나무, 대추나무에서만 나타났으며, 특히 고음나무에서 가장 높았다.

Keywords

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