Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Upregulation of heme oxygenase-1 by Scutellaria baicalensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells.

흰쥐 대뇌세포의 저산소증 모델에서 황금(黃芩)에 의한 heme oxygenase-1의 표현증가

  • Lee, Won-Chol (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Kim, Wan-Sik (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Shin, Gil-Jo (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Moon, Il-Soo (Department of Anatomy, College of Medicine, Dongguk University) ;
  • Jung, Seung-Hyun (Department of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 이원철 (동국대학교 한의과대학 내과학교실) ;
  • 김완식 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실) ;
  • 문일수 (동국대학교 의과대학 해부학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실)
  • Published : 2007.05.25
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Abstract

Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of heme oxygenase-1(HO-1), which has important biological roles in regulating mitochondrial heme protein turnover and in protecting against conditions such as hypoxia, neurodegenerative diseases, or sepsis. Rat cerebrocortical day In vitro(DIV)12 cells were grown in neurobasal medium. On DIV12 cells were treated with SB($20{\mu}g/ml$) and given a hypoxic shock ($2%\;O_2/5%\;CO_2,\;3\;hr$) on DIV14. In situ hybridization results revealed that SB upregulated HO-1 mRNA in neuronal dendrites in both normoxia and hypoxia(38.5% and 59.2%, respectively). At the protein level, SB upregulated HO-1 in the neuronal soma in both normoxia and hypoxia(22.4% and 15.7%, respectively). Interestingly, most significant increase was associated with astrocytes, which increased HO-1 protein by 77.5% compared to SB-untreated culture. These results indicate that SB upregulates both neuronal and glial HO-1 expression, which contributes to the neuroprotection efficacy in hypoxia).

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