Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative, Antimutagenic and Cytotoxic Effects of Natural Seasoning Using Lentinus edodes Powder

표고버섯 분말을 첨가한 천연 조미료 추출물의 항산화성, 항돌연변이성 및 세포독성 효과

  • Yoo, Su-Jung (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Soo-Hyun (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Houng-Taek (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Oh, Hyun-Taek (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Hyun-Jin (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Ham, Seung-Shi (School of Bioscience and Biotechnology, Kangwon National University)
  • 유수정 (강원대학교 바이오산업공학부 식품공학과) ;
  • 김수현 (강원대학교 바이오산업공학부 식품공학과) ;
  • 최형택 (강원대학교 바이오산업공학부 식품공학과) ;
  • 오현택 (강원대학교 바이오산업공학부 식품공학과) ;
  • 최현진 (강원대학교 바이오산업공학부 식품공학과) ;
  • 함승시 (강원대학교 바이오산업공학부 식품공학과)
  • Published : 2007.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was peformed to determine the antioxidative, antimutagenic and cytotoxic effects of the natural seasoning using Lentinus edodes powder (NSLP) by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical donating method, Ames test, and cytotoxicity, respectively. The scavenging effect on DPPH radical in ethyl acetate fraction of NSLP showed $155{\mu}g\;of\;RC_{50}$. The direct antimutagenic effects of ethanol extract and its solvent fractions of NSLP were examined by Ames test using Salmonella Typhimurium TA98 and TA100. In the Ames test, ethanol extract of NSLP alone did not exhibit any mutagenicity and most of the samples showed high antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitroso guanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). Ethyl acetate fraction of NSLP ($200{\mu}g/plate$) showed approximately 82% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain, whereas 84% and 80% inhibitions were observed on the mutagenesis induced by 4NQO and MNNG against TA100 strain. In anticancer effects of ethanol extract and its solvent fractions of NSLP against cancer cell lines including human lung carcinoma (A549), human breast adenocarcinoma (MCF-7), human hepatocellular carcinoma (Hep3B), human cervical adenocarcinoma (HeLa) and human gastric carcinoma (AGS) were investigated. The treatment of 1 mg/mL ethyl acetate fraction of NSLP showed strong cytotoxicity of 56.7%, 84.9%, 64.6%, 85.1% and 71.5% against A549, MCF-7, Hep3B, HeLa and AGS, respectively. In contrast 1 mg/mL treatment of NSLP extract and its solvent fractions had only $4{\sim}40%$ cytotoxicity on human transfomed primary embryonal kidney cell (293). From this result, it is suggested that NSLP is believed to have possible antioxidative, antimutagenic and anticancer capacities.

표고버섯 분말을 첨가한 천연조미료의 추출물 및 분획물에 대한 항산화 활성은 에틸아세테이트 분획물에서 $RC_{50}$값 $155{\mu}g$으로서 다른 시료보다 강한 항산화 활성을 나타내었다. 표고버섯 분말을 첨가한 천연조미료 추출물 및 그 분획물들의 항돌연변이 효과의 검토는 Salmonella Typhimurium의 변이주인 TA98과 TA100을 이용한 Ames test로 확인하였다. 그 결과 직접변이원인 MNNG에 대해 시료농도 $200{\mu}g/plate$에서 핵산과 에틸아세테이트 분획물은 TA100에서 $79{\sim}84%$의 강한 항돌연변이원성을 보였고 다른 시료에서도 65% 이상의 저해효과를 보였다. 4NQO에 대해서는 TA98과 TA100에서 에틸아세테이트 분획물이 가장 높은 저해효과를 나타내었다. 암세포 성장 억제효과를 검토한 결과 표고버섯 분말을 첨가한 천연조미료 에탄을 추출물 1 mg/mL 첨가 시 HeLa, Hep3B, MCF-7, AGS 및 A549에서 각각 83.3%, 61%, 81.3%, 58.9% 및 36.7%의 억제효과를 보였다. 표고버섯 분말을 첨가한 천연조미료 분획물에 대한 억제효과는 시료 농도 1 mg/mL 첨가 시 에틸 아세테이트 분획물의 경우 폐암세포인 A549 세포에 대하여 55.7%의 억제효과를 보인 것을 제외하고는 $71{\sim}85%$ 정도의 높은 억제효과를 보였으나 물 분획물의 경우 다른 추출물과 비교 시 암세포에 대해 상대적으로 낮은 억제효과를 나타내었다. 이러한 암세포에 대한 높은 억제효과에 비해 인간 신장 정상세포 293에 대한 시료를 첨가 시 40% 이하의 생육 억제율을 나타냄으로써 정상세포에 대해서는 낮은 독성효과를 가짐을 알 수 있었다.

Keywords

References

  1. Hong KH, Kim BY, Kim HK 2004. Studies on the biological activity of Pleurotus ferulea. J Korean Soc Food Sci Nutr 33: 791-796 https://doi.org/10.3746/jkfn.2004.33.5.791
  2. Roh SH. 2000. A study on baking white bread product deve lopment according to the amounts of mushroom powder added. Culinary Research 6: 281 -289
  3. Ji JH, Kim MN, Ham SS. 2000. Antimutagenic and cytotoxicity effects of Phellinus linteus extracts. J Korean Soc Food Sci Nutr 29: 322-328
  4. Zhang Y, Talalay P, Cho CG. 1992. An anticarcinogenic protective enzyme from brocoli. Proc Natl Acad Sci 90: 2399-2404
  5. Hong JS. 1980. Nutrition value and medicine efficacy of mushroom. Food Ind 53: 79-84
  6. Ko JW, Lee WY, Ha YS, Choi YH. 1999. Absorption characteristics of dried shiitake mushroom powder using different drying methods. Korean J Food Sci Technol 31: 128- 137
  7. Park MH, Oh KY, Lee BW. 1998. Anticancer activity of Lentinus edodes and Pleurotus ostreatus. Korean J Food Sci Technol 30: 702-708
  8. Hong JS, Lee KR, Kim YH, Kim DH, Kim MK, Lim YS, Yeo KY. 1988. Volatile flavor compounds of Korean shiitake mushroom (Lentinus edodes). Korean J Food Sci Technol 20: 606-612
  9. Choi MY, Lim SS, Chung TY. 2000. The effects of hot water soluble polysaccharides from Lentinus edodes on lipid metabolism in the rats fed butter yellow. J Korean Soc Food Sci Nutr 29: 294-299
  10. Lee SH, Park HJ, Cho SY, Jeong HJ. 2004. Supplementary effect of Lentinus edodes on serum and hepatic lipid levels in spontaneously hypertensive rat. Korean J Nutr 37: 509-514
  11. Kang MY, Kim S, Yun HJ, Nam SH. 2004. Antioxidative activity of the extracts from browned oak mushroom (Lentinus edodes) with unmarketable quality. Korean J Food Sci Technol 36: 648-654
  12. Kim SK, Byun HG, Jeon YJ, Joo DS, Kim JB. 1999. Development of natural seasoning using desalinated tuna boiled extract. J Korean Fish Soc 32: 75-82
  13. Choi JS, Park JH, Kim HG, Young HS, Mun SI. 1993. Screening for antioxidant activity of plants and marine algae and its active principle from Prunus daviana. Kor J Pharmacol 24: 299-303
  14. Yahagi T, Nagao M, Seino Y, Matsushima T, Sugimura T, Okada M. 1997. Mutagenicities of N-nitrosamines in Salmonella. Mutat Res 48: 121-130 https://doi.org/10.1016/0027-5107(77)90151-8
  15. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR. 1998. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48: 4827-4833
  16. Lee MH, Son HS, Choi OK, Oh SK, Kwon TB. 1994. Changes in physico-chemical properties and mineral contents during buckwheat germination. Korean J Food & Nutr 7: 267-273
  17. Oh SI, Lee MS. 2005. Antioxidative and antimutagenic effects of Ganoderma lucidum Krast extracts. Korean J Food & Nutr 18: 54-62
  18. Kim HJ, Lee IS. 2004. Antimutagenic and cytotoxic effects of Korean wild mushrooms extracts. Korean J Food Sci Technol 36: 662-668
  19. Kim HJ, Lee BH, Kim OM, Bae JT, Park DC, Lee KP. 1999. Antimutagenic effect of the fruting body and the mycelia extracts of Coprinus comatus. J Korean Soc Food Sci Nutr 28: 452-457
  20. Ji JH, Kim MN, Choi KP, Chung CK, Ham SS. 2000. Antimutagenic and cytotoxicity effects of Agaricus blazei Murill extracts. Korean J Food Sci Technol 32: 1371-1378
  21. Kim MN, OH SH, Lee DS, Ham SS. 2001. Antioxidative and antimutagenic effects of the ethanol extract from Cordyceps militaris. Korean J Food Preserv 11: 214-220
  22. Park SH, Kim OM, Lee KR. 2001. Antimutagenic and quinone reductase inducing activities of Hericium erinaceus extracts. J Korean Soc Food Sci Nutr 30: 1287-1292
  23. Kim HJ, Lee BH, Kim OM, Lee KD, Lee KR. 1998. Screening for antimutagenic effects of the wild mushrooms in Korea. Korean J Food Sci Technol 30: 688-692
  24. Ikekawa J, Nakamishi M, Uehara N, Chihara G, Fukuoka F. 1968. Antitumor action of some basidiomycetes especially Phellinus linteus. Gann 59: 155-157
  25. Chang ST, John AB, Chiu SW. 1993. Mushroom biology and mushroom products. World scientific, Washington DC. p 1-20
  26. Park JM, Lee SH, Kim JO, Park HJ, Park JB, Sin JI. 2004. In vitro and in vivo effects of extracts of Lentinus edodes on tumor growth in a human papillomavirus 16 oncogenes-transformed animal tumor model. Korean J Food Sci Technol 36: 141-146
  27. Chihara G, Hamuro J, Maeda Y, Arai Y, Fukuoka F. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk.) Sing. (an edible mushroom). Cancer Res 30: 2776-2781
  28. Kweon MH, Lim EJ, Sung HC. 1998. Studies on bioactive polysaccharide isolated from Agaricus bisporus. J Korean Soc Appl Biol Chem 41: 60-66

Cited by

  1. Analysis of Microbiological Contamination Levels of Cabbage and Fresh-cut Produce on Difference Area toward Climate in Korea vol.27, pp.3, 2012, https://doi.org/10.13103/JFHS.2012.27.3.209
  2. Antioxidant activity and quality characteristics on the maruration period of the soy sauce containing Astragalus memvranaceus and Oak mushroom (Lentinus edodes) vol.20, pp.4, 2013, https://doi.org/10.11002/kjfp.2013.20.4.467
  3. Conventional and Novel Applications of Edible Mushrooms in Today's Food Industry vol.38, pp.5, 2014, https://doi.org/10.1111/jfpp.12185
  4. Antioxidative and antimutagenic activities of 70% ethanol extract from masou salmon (Oncorhynchus masou) vol.22, pp.6, 2008, https://doi.org/10.1016/j.tiv.2008.05.002
  5. Optimum Formulation of Kochujang Seasoning Sauce with the Addition of Fruit and Vegetable Extract for Pork Bulgogi vol.37, pp.4, 2008, https://doi.org/10.3746/jkfn.2008.37.4.505
  6. 표고버섯가루 분말 첨가 식빵의 일반성분 및 품질 특성 vol.30, pp.6, 2007, https://doi.org/10.9799/ksfan.2017.30.6.1319
  7. Edible Mushrooms: A Comprehensive Review on Bioactive Compounds with Health Benefits and Processing Aspects vol.10, pp.12, 2007, https://doi.org/10.3390/foods10122996