Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Generation of a Transformant Showing Higher Manganese Peroxidase (Mnp) Activity by Overexpression of Mnp Gene in Trametes versicolor

  • Yeo, Su-Min (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University) ;
  • Park, Nam-Mee (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University) ;
  • Choi, Hyoung-T. (Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University)
  • Published : 2007.06.30
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Abstract

Trametes versicolor has a lignin degrading enzyme system, which is also involved in the degradation of diverse recalcitrant compounds. Manganese-dependent peroxidase (MnP) is one of the lignin degrading enzymes in T. versicolor. In this study, a cDNA clone of a putative MnP-coding gene was cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants were generated through genetic transformation using pBARTvMnP2. The genomic integration of the MnP clone was confirmed by PCR with bar-specific primers. One transformant showed higher enzyme activity than the recipient strain did, and was genetically stable even after 10 consecutive transfers on non-selective medium.

Keywords

References

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