Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Repeated Batch Production of Epothilone B by Immobilized Sorangium cellulosum

  • Park, Sang-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Park, Su-Jeong (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Han, Se-Jong (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • Kim, Dong-Shik (Department of Chemical and Environmental Engineering, University of Toledo) ;
  • Kim, Ji-Heung (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Kim, Byung-Woo (Department of Chemical Engineering, Sungkyunkwan University) ;
  • Lee, Jee-Won (Department of Chemical & Biological Engineering, Korea University) ;
  • Sim, Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University)
  • Published : 2007.07.31
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Abstract

Production of extracellular epothilone B, one of the potent anticancer agents, by free and immobilized Sorangium cellulosum was studied using the repeated batch culture process. The concentration of alginate used in immobilization was directly related to the mass transfer rate of nutrients, mechanical stability, and the epothilone B production yield. With the optimized 3% (w/v) calcium alginate carrier, a prolonged repeated batch culture was investigated for the 5 repeated batches for 24 days. The maximum productivity of epothilone B obtained from the alginate-immobilized cells was 5.03 mg/l/day, which is 3 times higher than that of free cells (1.68 mg/l/day).

Keywords

References

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