Changes in Isoflavone Content and Mass Balance During Soybean Processing

  • Han, Jin-Suk (Division of Food Science, Dong-Eui Institute of Technology) ;
  • Hong, Hee-Do (Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Sung-Ran (Food Function Research Division, Korea Food Research Institute)
  • Published : 2007.06.30

Abstract

We analyzed the isoflavone content of domestic soybeans during steaming, boiling, fermentation, germinating cultivation, fermentation, and soybean curd production. The isoflavone content of the beans was reduced by steaming and boiling, and overall reductions ranging from 16.0 to 65.0% of initial isoflavone values were detected. After 4 days of germinating cultivation, the total isoflavones of Eunhakong increased from 1,341 to $2,017\;{\mu}g/g$ and the total isoflavones of Guinunikong increased from 1,284 to $1,535\;{\mu}g/g$. The isoflavone content of the vinegar beans produced from Hwangkeumkong and Black No.1 increased from 1,877 to $1,956\;{\mu}g/g$, and from 885 to $1,956\;{\mu}g/g$ after 8 days of immersion in 4% acetic acid, respectively. During soybean curd production, significant amounts of isoflavones were lost in the whey (30-31 %) and soybean curd residue (15-20%). Only 37.4% of the isoflavones present in the original soybeans remained in the soybean curd with the hot extraction method, and 50.7% of them with the cold extraction method. Soybean curd prepared with whole soybean method, however, retained 80.7% of the initial isoflavones.

Keywords

References

  1. Hendrich SK, Lee W, Xu X, Wang HJ, Murphy PA. Defining food components as new nutrient. J. Nutr. 124: 1789S-1792S (1994) https://doi.org/10.1093/jn/124.6.789
  2. Choi MS, Rhee KC. Production and processing of soybeans and nutrition and safety of isoflavone and other soy products for human health. J. Med. Food 9: 1-10 (2006) https://doi.org/10.1089/jmf.2005.055
  3. Mazur WM, Duke JA, Wahala K, Rasku S, Adlerecreutz H. Isoflavonoids and lignans in legumes: Nutritional and health aspects in humans. J. Nutr. Biochem. 9: 193-200 (1998) https://doi.org/10.1016/S0955-2863(97)00184-8
  4. Messina M. Modem applications for an ancient bean: Soybeans and the prevention and treatment of chronic disease. J. Nutr. 125: 567S-569S (1995)
  5. Messina M, Messina V. Increasing use of soyfoods and their potential role in cancer prevention. J. Am. Diet. Assoc. 91: 836-840 (1991)
  6. Sarkar FH, Li Y. Soy isoflavones and cancer prevention. Cancer Invest. 21: 817-818 (2003) https://doi.org/10.1081/CNV-120023782
  7. Messina M, Persky V, Setchell KDR, Branes S. Soy intake and cancer risk: A review of the in vitro and in vivo data. Nutr. Cancer 21: 113-131 (1994) https://doi.org/10.1080/01635589409514310
  8. Tikkanen MJ, Adlercneutz H. Dietary soy-derived isoflavone phytoestrogen. Could they have a role in coronary heart disease prevention? Biochem. Pharmacol. 60: 1-5 (2000) https://doi.org/10.1016/S0006-2952(99)00409-8
  9. Fonseca D, Ward WE. Daidzein together with high calcium preserve bone mass and biochemical strength at multiple sites in ovariectomized mice. Bone 35: 489-497 (2004) https://doi.org/10.1016/j.bone.2004.03.031
  10. Peterson G, Barnes S. Genistein inhibition of the growth of human breast cancer cell: Independence from estrogen receptors and the multidrug resistance gene. Biochem. Bioph. Res. Co. 179: 661-667 (1991) https://doi.org/10.1016/0006-291X(91)91423-A
  11. Barnes ST, Peterson G, Grubbs C, Setchell KDR, Caison J. Soybeans inhibit mammary tumors in models of breast cancer. pp 239-253. In: Mutagens and Carcinogens lin the Diet. Pariza MD (ed). Wiley-Liss, New York, NY, USA (1990)
  12. Coward L, Bames NC, Setchell KDR, Barnes S. Genistein, daidzein and their $\beta$-glucoside conjugates: Antitumor isoflavones in soybean foods from American and Asian diets. J. Agr. Food Chem. 31: 394-396 (1993) https://doi.org/10.1021/jf00116a052
  13. Barnes S, Kirk M, Coward L. Isoflavones and their conjugates in soy foods: Extraction conditions and analysis by HPLC-mass spectrometry. J. Agr. Food Chem. 42: 2466-2474 (1994) https://doi.org/10.1021/jf00047a019
  14. Jackson CJC, Dini JP, Lavandier C, Rupasinghe HPV, Faulkner H, Poysa V, Buzzel D, DeGrandis S. Effects of processing on the content and composition of isoflavones during manufacturing of soy beverage and tofu. Process Biochem. 37: 1117-1123 (2002) https://doi.org/10.1016/S0032-9592(01)00323-5
  15. Wang HJ, Murphy PA. Mass balance study of isoflavones during soybean processing. J. Agr. Food Chem. 44: 2377-2383 (1996) https://doi.org/10.1021/jf950535p
  16. Wang H, Murphy PA. Isoflavone content in commercial soybean foods. J. Agr. Food Chem. 42: 1674-1677 (1994) https://doi.org/10.1021/jf00044a017
  17. Moon BK, Jeon KS, Hwang IK. Isoflavone contents in some varieties of soy and on processing conditions. Korean J. Soc. Food Sci. 12: 524-527 (1996)
  18. Wang C, Ma Q, Pagadala S, Sherrad MS, Krishnan PG. Changes of isoflavones during processing of soy protein isolates. J. Am. Oil Chem. Soc. 75: 337-341 (1998) https://doi.org/10.1007/s11746-998-0050-7
  19. Kim WJ, Lee HY, Yoo SH. Germination effect of soybean in its contents of isoflavones and oligosaccharides. Food Sci. Biotechnol. 14: 498-502 (2005)
  20. Bang HY, Kim GH. A study on the quality characteristics of doenjang prepared with Paecilomyces japonica, from silkworm. Korean J. Soc. Food Cookery Sci. 19: 694-700 (2003)
  21. Han JS, Kim MR. Effects of chitooligosaccharide on the physicochemical, textural, and sensory properties of tofu. J. Texture Stud. 33: 1-14 (2002) https://doi.org/10.1111/j.1745-4603.2002.tb01331.x
  22. Wang G, Kuan SS, Fransis OJ, Ware GM, Carman AS. A simplified HPLC method for the determination of phytoestrogens in soybean and its processed products. J. Agr. Food Chem. 38: 185-190 (1990) https://doi.org/10.1021/jf00091a041
  23. Grun IU. Adhikari K, Li C, Lin B, Zhang J, Fernando LN. Changes in the profile of genistein, diadzein, and their conjugates during thermal processing of tofu. J. Agr. Food Chem. 49: 2839-2843 (2001) https://doi.org/10.1021/jf010028+
  24. Mostafa MM, Rahma EH, Rady AH. Chemical and nutritional changes in soybeans during germination. Food Chem. 23: 257-275 (1987) https://doi.org/10.1016/0308-8146(87)90113-0
  25. Kim WJ, Lee HY, Won MH, Yoo SH. Germination effect of soybean on its isoflavones and oligosaccharides. Food Sci. Biotechnol. 14: 498-502 (2005)
  26. Bang HP, Choi OK, Cho GS, Son JY, Ryu GH. The change of compositions and antioxidant effect in soybean cultivars pickled in persimmon vinegar. Korean J. Food and Nutr. 19: 398-409 (2006)
  27. Jang CH, Lim JK, Kim JH, Park CS, Kwon DY, Kim YS, Shin DH, Kim JS. Change of isoflavone content during manufacturing of chunggukjang, a traditional Korean fermented soyfood. Food Sci. Biotechnol. 15: 643-646 (2006)
  28. Shimoni E. Stability and shelf life of bioactive compounds during food processing and storage: Soy isoflavones. J. Food Chem. 69: 160-166 (2004)