Journal of Asia-Pacific Entomology

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Larvicidal Efficiency of Certain Seed Extracts Against Anopheles Stephensi, with Reference to Azadirachta indica

  • Batabyal, Lata (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science Dayalbagh Educational Institute (Deemed University)) ;
  • Sharma, Preeti (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science Dayalbagh Educational Institute (Deemed University)) ;
  • Mohan, Lalit (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science Dayalbagh Educational Institute (Deemed University)) ;
  • Maurya, Prejwltta (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science Dayalbagh Educational Institute (Deemed University)) ;
  • Srivastava, C.N. (Applied Entomology & Vector Control Laboratory, Department of Zoology, Faculty of Science Dayalbagh Educational Institute (Deemed University))
  • Published : 2007.09.30
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Abstract

The toxicity of seed extracts of three Indian medicinal plants, Azadirachta indica, Momordica charantia and Ricinus communis, was evaluated for their larvicidal efficacy against Anopheles stephensi. The methanol extract of A. indica exhibited potent larvicidal activity with $LC_{50}$ 15.25 and 12.70 ppm and $LC_{90}$ 46.79 and 45.56 ppm after 24 and 48 hrs, respectively, followed by methanol extract of R. communis with $LC_{50}$ 54.95 and 23.06 ppm and $LC_{90}$ 251.03 and 144.54 ppm after 24 and 48 hrs of post treatment, respectively. In case of M. charantia, the carbon tetrachloride extract possess potential larvicidal efficacy with $LC_{50}$ values 87.00 and 57.53 ppm and $LC_{90}$ 301.20 and 262.21 ppm after 24 and 48 hrs of exposure period. The results indicate that A. indica methanol extract was most potential mosquito larvicide and can be use as alternate potential to synthetic insecticides.

Keywords

References

  1. Abbot, W.S. 1925. A method of computing the effectiveness of an insecticides. J. Econ. Entomol. 8: 265-267
  2. Adebayo, T.A., A.A. Gbolade and J.I. Olaifa. 1999. Comparitive study of toxicity of essential oils to larvae of three mosquito species. Nigerian J. Nat. Prod. Med. 3: 74-76
  3. Aliero, B.L. 2003. Larvicidal effects of aqueous extracts of Azadirachta indica (neem) on the larvae of Anopheles mosquito. American. J. Biotech. 2(9): 325-327
  4. Ciccia, G., J. Coussio and I. Mongelli. 2000. Insecticidal activity against Aedes aegypti larvae of some medicinal South American plants. J. Ethno. 72: 185-189 https://doi.org/10.1016/S0378-8741(00)00241-5
  5. EI Hag, E.A., A.H. Nadi and A.A. Zaitoon. 1999. Toxic and growth retarding effects of three plant extracts against Culex pipiens larvae (Diptera: Culicidae), Phytother. Res. 13: 388-392 https://doi.org/10.1002/(SICI)1099-1573(199908/09)13:5<388::AID-PTR455>3.0.CO;2-U
  6. EI Hag, E.A., Abd-Ei Rahman, H. EI Nadi and A.A. Zaitoon. 2001. Effects of methanolic extracts of neem seeds on egg hatchability and larval development of Culex pipiens mosquitoes. Indian Vet. J. 78: 199-201
  7. Finney, D.J. 1971. Probit Analysis. 3rd Edn. Cambridge University, Cambridge
  8. Gbolade, A.A., A.O. Oyedele, M.B. Sosan, I.B. Adewayan and O.L. Soyela. 2000. Mosquito repellent activities of essential oils from two Nigerian Ocimum species. J. Trop. Med. Plants, 1: 146-148
  9. Hussein, Z.A. M., I.B. Jantan, A Awaludin and N.W. Ahmad. 2002. Mosquito repellent activities of the methanol extracts of some Ascidian species. Pharm. Biol. 40: 358-361 https://doi.org/10.1076/phbi.40.5.358.8456
  10. Isman, M.B. 2000. Plant essential oils for pest and disease management. Crop Prot. 19: 603-608 https://doi.org/10.1016/S0261-2194(00)00079-X
  11. Jeyabalan, D., N. Arul and P. Thangamathi. 2003. Studies on effects of Pelargonium citrosa leaf extracts on malaria vector, Anopheles stephensi Liston. Biores. Teechnol. 89(2): 185-189 https://doi.org/10.1016/S0960-8524(03)00036-1
  12. Keita, S.M., C. Vincent, J.P. Schmit, J.T. Arnason and A. Belanger. 2001. Efficacy of essential oils of Ocimum basilicum L. and Ocimum gratissimum L. applied as an insecticidal fumigants and powder control against Callosobruchus maculates (Fab.) [Coleoptera: Bruchidae]. J. Stored Prod. Res. 37: 339-349 https://doi.org/10.1016/S0022-474X(00)00034-5
  13. Manish, N., C. Bapu and P. Harish (2001). Alpha-terthienyl: A plant derived new generation insecticide. Curr. Sci. 81(6): 667-672
  14. Mittal, P.K. and S.K. Subbarao. 2003. Prospects of using herbal products in the control of mosquito larvae. ICMR Buuletin, 33(1): 1-10
  15. Mohan, L., P. Sharma and C.N. Srivastava. 2005. Evaluation of Solanum xanthocapum extract as mosquito larvicides. J. Environ. Biol. 26: 399-401
  16. Mohan, L., P. Sharma and C.N. Srivastava. 2006. Evaluation of Solanum xanthocarpum extract as a synergist for cypermetrin against larvae of the filarial vector, Culex quin- quefasciatus (Say). Entomol. Res. 36: 220-225 https://doi.org/10.1111/j.1748-5967.2006.00037.x
  17. Moore, S.A., A. Lenglet and N. Hill. 2002. Field evaluation of three plant based insect repellents against malaria vectors in VACAdiE2 Province of the Bollivian Amazon. J. Am. Mosq. Cont. Assoc. 18: 107
  18. Nagpal, B.N., A. Srivastava, N. Valeena and V.P. Sharma. 2001. Repellent action of neem cream against Anopheles culicifacies and Culex quinquefasciatus. Curr. Sci. 80: 1270
  19. Neraliya, S. and U.S. Srivastava. 1996. Effects of plant extrats on the post embryonic development of the mosquito Culex quinquefasciatus. J. Adv. Zool. 17(1): 54-58
  20. RajKumar, S. and A. Jebanssan. 2004. Mosquitocidal activities of octacosane from Moschosma polystachyum Linn. (Lamiaceae), J. Ethnopharmacol. 90: 87-89 https://doi.org/10.1016/j.jep.2003.09.030
  21. Rao, D.R., M.S. Venugopal, B.A. Nagasampgi and H. Schmutterer. 1992. Evaluation of neem- Azadirachta indica with and without water management for the control of culicine mosquito larvae in rice field. Med. Vet. Entomol. 6: 318
  22. Sagar, S.K. and S.S. Sehgal. 1996. Effects of aqueous extract of deoiled neem (Azadirachta indica) seed kernel and Karonja (Pongamia globera) seed kernel against Culex quinquefasciatus. J. Commun. Dis. 28: 260
  23. Sakthivadivel, M. and D. Thilagavathy. 2003. Larvicidal and chemosterilant activities of the acetone fraction of petroleum ether extract from Aregemone maxicana L. seed. Biores. Technol. 89(2): 213-216 https://doi.org/10.1016/S0960-8524(03)00038-5
  24. Saxena, R.C., S. Jayashree, S. Padma and O.P. Dixit. 1994. Evaluation of growth disrupting activity of Ageratum conyzoides crude extract on Culex quinquefasciatus (Diptera: Culicidae). J. Environ. Biol. 15: 67-74
  25. Sharma, P., L. Mohan and C.N. Srivastava. 2004. Larval susceptibility of Ajuga remota against anopheline and culicine mosquitoes. Southeast Asian J. Trop. Med. Public Health. 35: 608-610
  26. Sharma, P., L. Mohan and C.N. Srivastava. 2005. Larvicidal potential of Nerium indicum and Thuja orientelis extracts against malaria and Japanese encephalitis vector. J. Environ. Biol. 26(4): 657-660
  27. Sharma, P., L. Mohan and C.N. Srivastava. 2006. Impact analysis of Neem kernel extract on the developmental profile of Anopheles stephensi. J. Asia Pacific Entomol. 9(1): 11-17 https://doi.org/10.1016/S1226-8615(08)60270-8
  28. Sreenivasa, D.R., S. Sridhar and P. Suresh. 2002. Mosquito repellent properties of a high polar extract of neem seed kernel. World Neem Conference, Neem Proceedings
  29. Srivastava, U.S. and S. Neraliya. 1997. Larvicidal activity of plant extracts on filarial mosquito Culex quinquefasciatus. Proceedings of the National Academy of Sciences, India Section B, Biol. Sci. 67(2): 111-115
  30. Sukumar, K., M.J. Perich and L.R. Boobar. 1991. Botanical derivatives in mosquito control; a review. J. Am. Mosq. Cont. Assoc. 72: 210-237
  31. Templeton, W. 1969. An introduction to the chemistry of terpenoides and steroids. Butterworth, London
  32. Tripathi, A.K., V. Prajapati, K.K. Agarwal, S.P.S. Khanuja and S. Kumar. 2000. Repellency and toxicity of oil from Artemisia annua to certain stored product beetles. J. Econ. Entomol. 93: 43-47 https://doi.org/10.1603/0022-0493-93.1.43
  33. World Health Organization. 1975. Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. Mimeographed Document WHO/VBC/75-583