KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Antioxidant Activity and Cytotoxicity on Human Cancer Cells of Anthocyanin Extracted from Black Soybean

검정콩 안토시아닌의 항산화 및 암세포독성

  • Published : 2008.12.31
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Abstract

Anthocyanin pigments in soybean seed coat were D3G (Delphinidin-3-glucoside), C3G (Cyanidin-3- glucoside) and Pt3G (Petunidin-3-glucoside), which have been known potential roles in the prevention and treatment of chronic diseases. Anthocyanin contents in seed coat of blck soybean were significantly different according to soybean variety, C3G content showed the highest value in all materials and its variation was also wide. Antioxidant activity of each pigment was analyzed by DPPH and TEAC methods in which D3G and C3G showed high activity. And this study was carried out to investigate the effects of anthocyanin to human cancer cells. Cytotoxity were analyzed by MTT assay after anthocyanin pigments treated on leukemia (Jurkat T) and adenocarsinoma (MCF-7) cells. It showed decrement of cell numbers as anthocyanin concentration is increasing. ${EC}_50$ range of anthocyanin concentrations were $100{\sim}250\;ug/mL$ and $100{\sim}250\;ug/mL$ in Jurkat T and MCF-7 cell, respectively. D3G showed higher cytotoxicity than other pigments in Jurkat T cell whereas activity of C3G was high in MCF-7 cell. It is believed that supplementation of human diets with soybean anthocyanin markedly reduces human cancer mortality rates.

검정콩 안토시아닌의 항산화력과 암세포독성을 분석한 결과는 다음과 같다. 1. 안토시아닌 함량은 일품검정콩이 공시재료 중 가장 높게 나타났으며, 특히 C3G 함량은 타 계통보다 2배 이상 높았다. 2. 안토시아닌을 분획한 후 각각의 pigment로 항산화 효과를 분석한 결과 TEAC 법과 DPPH법 모두 C3G, D3G, 및 Pt3G의 효과가 인정되었으며, C3G와 D3G의 항산화력이 높게 나타났다. 일품검정콩과 재래속청간 품종 비교에서는 일품검정콩이 3가지 분획 모두 재래속청 보다 항산화 효과가 높았으며, 이는 TEAC 법과 DPPH법 공히 같은 경향이었다. 3. 인간 암세포인 Jurkat T 세포와 MCF-7 세포에 안토시 아닌 개별색소(C3G, D3G 및 Pt3G)를 $100{\sim}500\;{\mu}g/mL$ 농도로 처리하고 암세포에 대한 독성을 관찰한 결과, 3가지 시험물질 모두 두 가지 암 세포에서 세포독성 효과를 나타내었다. 이와 같은 결과는 검정콩 안토시아닌이 여러 가지 생리활성 효과를 가지고 있음을 나타내는 결과로 판단된다.

Keywords

References

  1. Blois, M. S. 1958. Antioxidant determinations by the use of stable free radical. Nature 181 : 1199-1200 https://doi.org/10.1038/1811199a0
  2. Harborne, J. B. 1988. Introduction to ecological biochemistry. 3rd ed. Academic press. London
  3. Harborne, J. B. and C. A. Wiliam. 2000. Advances in flavonoid research since 1992. Phytochemistry. 55 : 481-504 https://doi.org/10.1016/S0031-9422(00)00235-1
  4. Kanatt, S. R., R. Chander, P. Radhakrishna, and A. Sharma. 2005. Potato peel extract a natural antioxidant for retarding lipid peroxidation in radiation processed lamb meat. J. Agric. Food Chem. 53 : 1499-1504 https://doi.org/10.1021/jf048270e
  5. Kim, Y. H., J. H. Lee, Y. S. Lee, and H. T. Yun. 2006. Antioxidant activity and extraction efficiency of anthocyanin pigments in black colored soybean. K. Soybean Digest 23(1) : 1-9
  6. Kong, J. M., L. S. Chia, N. K. Goh, T. F. Chia, and R. Brouillard. 2003. Analysis and biological activities of anthocyanins. Phytochemistry 64(5) : 923-933 https://doi.org/10.1016/S0031-9422(03)00438-2
  7. Meiers, S., M. Kemeny, U. Weyand, R. Gastpar, E. von Angerer, and D. Marko. 2001. The anthocyanidins cyanidin and delphinidin are potent inhibitors of the epidermal growthfactor receptpr. J. Agric. Food Chem. 49 : 958-962 https://doi.org/10.1021/jf0009100
  8. Miller, N. J., C. A. Rice-Evans, M. J. Davies, V. Gopinathan, and A. Milner. 1993. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Sci. 84 : 407-412 https://doi.org/10.1042/cs0840407
  9. Plochmann, K., G. Korte, E. Koutsilieri, E. Richling, P. Riederer, A. Rethwilm, P. Schreier, and C. Scheller. 2007. Structure-activity relationships of flavonoid-induced cytotoxicity on human leukemia cells. Arch Biochem Biophys., 460(1) : 1-9 https://doi.org/10.1016/j.abb.2007.02.003
  10. Prior, R. L., X. Wu, and K. Schaich. 2005. Standardized method for determination of antioxidant capacity and phenolics in foods and biological and food samples. J. Agric. Food Chem. 53 : 4290-4302 https://doi.org/10.1021/jf0502698
  11. Tsuda, T., F. Horio, Kitoh, J., and T. Osawa. 1999. Protective effects of dietary cyanidin-3-O-beta-glucoside on liver Ischemiareferfusion injury in rats. Archives. Biochem. Biophys. 368(2) : 361-366 https://doi.org/10.1006/abbi.1999.1311
  12. Wang, S. Y. and H. S. Lin. 2000. Antioxidant activity in fruits and leaves of blackberry, raspberry, and straeberry varies with cultivar and developmental stage. J. Agric. Food Chem. 48 : 140-146 https://doi.org/10.1021/jf9908345
  13. Yang, C. S., J. M. Landau, M. T. Huang, and H. L. Newmark. 2001. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu. Rev. Nutr. 21 : 381-406 https://doi.org/10.1146/annurev.nutr.21.1.381
  14. 김선희, 권태완, 이영순, 정명근, 문갑순. 2005. 검정콩의 주요 항산화 원인 물질 및 항산화 효과 비교. 한국식품과학회지 37(1) : 73-77