Abstract
This study was conducted to clarify the effect of light-emitting diode (LED) for the light quality on the germination and physiological activity of amaranth (Amaranthus spp.) sprouts. We have germinated and grown the amaranth sprouts with blue, green, red, white, yellow, red+blue color lights for 14 hours and the dark condition for 10 hour, at the temperature of 25℃ (daytime) / 18℃ (nighttime). We have also made amaranth sprout extracts by methanol and examined into their physiological activities. All seed germination of amaranth sprouts were completed within 3 days after seeding irrespective of light sources. The fresh weight of total sprout has been increased in amaranth sprouts, which showed no significant meaning among the different treatments. However, the physiological activities indicated a significance meaning among the different light quality treatments. The total phenol compound contents were extremely increased in amaranth sprouts grown under the blue color light and the mixed light of blue and red color. And the total flavonoid contents were significantly increased under the red+blue light by 21.2 ㎎·L-1 which increased twice compared with the control. DPPH radical scavenging activity at 2,000 ㎎·L-1 were increased over 34% which were sprouted and grown under the blue or mixed light of red and blue color compared with the control. Nitrite radical scavenging activities of sprouts were most decreased compared with the control when grown under all lights except yellow light. Mushroom tyrosinase inhibition activity of amaranth sprouts was much lower than the control under the blue and mixed light of blue and red color.
아마란서스의 새싹 발아와 생리활성에 효과적인 영향을 미치는 LED 광질 종류를 구명하기 위해 청색, 녹색, 적색, 백색,황색,적색+청색광을 발광14시간,암조건10시간,주간 25℃,야간18℃로 조절하여 종자 발아와 새싹을 생장을 시켰다. 생리활성 조사는 새싹을 메탄올로 추출한 것을 이용하여 실시하였다. 종자 발아율과 발아속도는 광질에 관계없이 3일만에 100% 발아되었고, 파종 후 6일째의 신선중도 처리구간 유의적인 차이가 보이지 않았다. 그러나 광질에 따른 생리활성 물질 함량에는 차이가 나타났는데, 새싹의 총페놀함량은 청색광과 청색+적색광에서 대조구보다 높게 나타났으며,총플라보노이드 함량도 적색+청색 광처리구에서 21.2㎎·L-1로 대조구보다2배 높았다.전자공여능은 추출물 2,000㎎·L-1일 때 청색과 적색+청색 광처리구에서 34% 이상으로 대조구보다 높게 나타났다. 아질산염 소거능은 황색광을 제외한 모든 광질에서 대조구보다 낮게 나타났다. Tyrosinase 저해 활성은 청색과 청색+적색광에서 오히려 대조구보다 낮게 나타났다. 이와 같이 아마란서스 새싹의 생리활성은 LED 광질에 따라 차이가 있었으며, 적용시는 이를 고려하는 것이 좋을 것으로 판단된다.