Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Comparison of Biological Activities of Extracts from Different Parts and Solvent Fractions in Cornus kousa Buerg

산딸나무의 부위별 추출물 및 용매 분획물의 생리활성 비교

  • Kim, Young-Jung (Dept. of Horticultural Science, Chungbuk National Univ.) ;
  • Jeong, Jin-A (Dept. of Horticultural Science, Chungbuk National Univ.) ;
  • Kwon, Su-Hyun (Dept. of Horticultural Science, Chungbuk National Univ.) ;
  • Lee, Cheol-Hee (Dept. of Horticultural Science, Chungbuk National Univ.)
  • Published : 2008.02.27
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Abstract

Several extracts of different parts and solvent fractions of Cornus kousa were obtained and their functional material contents, antioxidant activities and tyrosinase inhibition effects were determined. Content of total polyphenols and flavonoids contents in flower were 169.638 $mg{\cdot}g^{-1}$ and 25.418 $mg{\cdot}g^{-1}$, respectively, which were much higher than those of other parts. Also, flower extracts showed the strongest effects on DPPH and ABTS radicals scavenging and ferrous ion chelating. In flower, leaf, and stem extracts, inhibition effects on peroxidation of linoleic acid determined by ferric thiocyanate(FTC) method were higher than a synthetic antioxidant, BHT. Tyrosinase inhibition activities were shown only in flower extract. Flower and leaf extracts, showing high biological activities in various system, were successively reextracted with n-hexane, chloroform, ethylacetate and n-butanol. Total polyphenol contents of water fractions were higher than any other solvent fractions in both flower and leaf, 67.006 $mg{\cdot}g^{-1}$ and 67.739 $mg{\cdot}g^{-1}$, respectively. But total flavonoid contents were higher in ethyl acetate fraction for flower extract and butanol fraction for leaf extract. Among the solvent fractions, the highest efficiency of free radical scavenging activities was obtained in ethyl acetate fraction for flower extract and n-butanol fraction for leaf extract. Tyrosinase inhibition activities were higher in water fraction for both flower and leaf extracts, 49.24% and 31.8%, respectively.

산딸나무의 꽃, 잎, 열매, 가지 등 부위별 추출물과 용매 분획물을 제조하여 각각의 생리활성물질 함량, 항산화활성 그리고 tyrosinase 억제활성을 분석하였다. 총 폴리페놀 및 플라보노이드 함량은 다른 부위보다 꽃 추출물에서 169.638 $mg{\cdot}g^{-1}$과 25.418 $mg{\cdot}g^{-1}$으로 가장 높게 나타났다. DPPH 및 ABTS 라디칼 소거활성과 $Fe^{2+}$ chelating 효과 역시 꽃 추출물에서 가장 우수한 것으로 조사되었다. Ferric thiocyante 방법으로 지질 과산화 억제효과를 조사한 결과, 꽃, 잎, 가지의 경우는 BHT보다 높은 억제활성을 보였다. Tyrosinase 억제활성은 오직 꽃 추출물에서만 나타났다. 높은 생리활성을 보인 꽃과 잎 추출물을 n-hexane, chloroform, ethyl acetate, n-butanol의 순으로 용매분획한 결과, 폴리페놀함량은 꽃과 잎 모두 water 분획물에서 각각 67.006 $mg{\cdot}g^{-1}$ 및 66.739 $mg{\cdot}g^{-1}$으로 가장 많았다. 반면 플라보노이드 함량은 꽃의 경우 ethyl acetate 분획물에서 가장 높았고, 잎은 butanol 분획물에서 가장 높게 조사되었다. 자유라디칼 소거활성은 꽃 추출물의 경우 ethyl acetate 분획물에서 가장 높았고 잎 추출물은 butanol 분획물에서 가장 높게 조사되었다. 꽃과 잎의 각 용매 분획물이 tyrosinase의 활성에 미치는 영향을 조사한 결과, 모두 water 분획물에서 각각 49.24% 및 31.8%로 가장 높은 억제 효과를 보였다.

Keywords

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